公开(公告)号：EP1985700A4

公开(公告)日：2009-08-12

申请号：EP07707937

申请日：2007-02-02

申请人： KANEKA CORP

发明人： MORIYAMA DAISUKE ,  TAOKA NAOAKI

IPC分类号： C12N15/00 ,  C12N1/21 ,  C12N9/04 ,  C12N15/09 ,  C12P7/02 ,  C12P41/00 ,  C12R1/01

CPC分类号： C12Y101/01304 ,  C12N9/0006 ,  C12P7/18 ,  C12P41/002

摘要： Disclosed are: a novel (S,S)-butanediol dehydrogenase; a gene encoding the enzyme protein; a vector carrying the gene; a transformant having the vector therein; and a method for production of an optically active alcohol using the transformant. The polypeptide has physicochemical properties including a property of reacting, together with NAD+ as a coenzyme, with (2S,3S)-2,3-butanediol to produce (S)-acetoin and a property of reducing, together with NADH as a coenzyme, 2,3-butandione to produce (2S,3S)-2,3-butanediol.

公开(公告)号：EP2039689A4

公开(公告)日：2011-12-28

申请号：EP07743869

申请日：2007-05-22

申请人： KANEKA CORP

发明人： MORI KOHEI ,  NISHIYAMA AKIRA ,  TAOKA NAOAKI ,  MORIYAMA DAISUKE ,  NAGASHIMA NOBUO

IPC分类号： C07D263/16 ,  C07B53/00 ,  C07B61/00 ,  C07C67/31 ,  C07C69/675 ,  C07C231/12 ,  C07C231/16 ,  C07C237/04 ,  C07C237/22 ,  C07D301/24 ,  C07D303/16 ,  C07D303/48 ,  C12P7/42 ,  C12P7/62

CPC分类号： C07B53/00 ,  C07B2200/07 ,  C07C67/31 ,  C07C231/18 ,  C07C237/04 ,  C07C237/22 ,  C07C2601/02 ,  C07D263/16 ,  C07D301/24 ,  C07D303/16 ,  C07D303/48 ,  C12P7/42 ,  C12P13/02 ,  C12P41/002 ,  C07C69/675

公开(公告)号：EP1811021A4

公开(公告)日：2008-05-28

申请号：EP05795588

申请日：2005-10-20

申请人： KANEKA CORP

发明人： KIZAKI NORIYUKI ,  UEDA MAKOTO ,  MORIYAMA DAISUKE ,  TAOKA NAOAKI ,  YASOHARA YOSHIHIKO

IPC分类号： C12N15/00 ,  C07K14/40 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/02 ,  C12N15/09 ,  C12P41/00

CPC分类号： C12N9/0006 ,  C12P7/04 ,  C12P7/62 ,  C12P13/002 ,  C12P41/002

摘要： An efficient process for producing optically active alcohols, such as (R)-3-hydroxy-3-phenylpropanenitrile. In one aspect, there is provided a polypeptide isolated from a microbe belonging to the genus Candida, having the activity of asymmetric reduction of 3-oxo-3-phenylpropanenitrile into formation of (R)-3-hydroxy-3-phenylpropanenitrile, and further provided a DNA coding for the polypeptide and a transformant capable of producing the polypeptide. In another aspect, there is provided a process for producing an optically active alcohol, such as (R)-3-hydroxy-3-phenylpropanenitrile, comprising reducing a carbonyl compound, such as 3-oxo-3-phenylpropanenitrile, by the use of the above polypeptide or transformant.

公开(公告)号：EP2128258A4

公开(公告)日：2011-01-26

申请号：EP08722100

申请日：2008-03-14

申请人： KANEKA CORP

发明人： NOJIRI MASUTOSHI ,  MORIYAMA DAISUKE ,  NISHIYAMA TOZO ,  TAOKA NAOAKI

IPC分类号： C12N15/52 ,  C12N1/21 ,  C12N9/86 ,  C12P41/00

CPC分类号： C12N9/80 ,  C12P13/02 ,  C12P41/006

公开(公告)号：EP1854893A4

公开(公告)日：2008-06-18

申请号：EP06714508

申请日：2006-02-24

申请人： KANEKA CORP

发明人： IWASAKI AKIRA ,  WASHIDA MOTOHISA ,  TAOKA NAOAKI ,  MORIYAMA DAISUKE ,  HASEGAWA JUNZOU

IPC分类号： C12P7/04 ,  C12P7/16 ,  C12P7/24 ,  C12P7/40 ,  C12P7/56 ,  C12P41/00

CPC分类号： C12P41/002 ,  C12N9/0008 ,  C12N9/0036 ,  C12P7/02 ,  C12P7/18

摘要： The present invention provides a method enabling convenient production of an optically active secondary alcohol useful as a pharmaceutical intermediate, particularly an optically active 1,2-diol and an optically active 2-alkanol, from an enantiomer mixture thereof. An oxidizing enzyme source having the capability of selectively oxidizing one enantiomer of secondary alcohol is allowed to act on an enantiomer mixture of secondary alcohol in the presence of a reducing enzyme source having the capability of reverse enantio-selectively reducing a ketone derivative, to convert the enantiomer mixture into a substantially single enantiomer at a theoretical percent recovery of 100%, whereby an optically active secondary alcohol is produced.

公开(公告)号：EP2527436A4

公开(公告)日：2013-09-18

申请号：EP11734660

申请日：2011-01-19

申请人： KANEKA CORP

发明人： YOSHIDA SHINICHI ,  IWASAKI AKIRA ,  WASHIDA MOTOHISA ,  NISHIYAMA TOZO ,  MORIYAMA DAISUKE ,  TAOKA NAOAKI

IPC分类号： C12N15/09 ,  C12N1/15 ,  C12N1/19 ,  C12N1/21 ,  C12N5/10 ,  C12N9/04

CPC分类号： C12N9/0036 ,  C12N9/0065 ,  C12P7/02 ,  C12P7/18 ,  C12P7/42 ,  C12P13/04 ,  C12P19/36 ,  C12Y106/03 ,  C12Y106/03001 ,  C12Y111/01001

公开(公告)号：EP1568679A4

公开(公告)日：2012-08-29

申请号：EP03777330

申请日：2003-12-08

申请人： KANEKA CORP

发明人： TAOKA NAOAKI ,  MORIYAMA DAISUKE ,  MORI KOHEI ,  OISHI TAKAHIRO

IPC分类号： C07C67/36 ,  C07C67/313 ,  C07C69/716 ,  C07D317/60 ,  C12P7/62 ,  C12P17/04 ,  C12P41/00

CPC分类号： C12P7/62 ,  C07C67/313 ,  C07D317/60 ,  C12P17/04 ,  C12P41/002 ,  C07C69/716

公开(公告)号：EP1762621A4

公开(公告)日：2007-09-12

申请号：EP05751178

申请日：2005-06-15

申请人： KANEKA CORP

发明人： MORIYAMA DAISUKE ,  TAOKA NAOAKI

IPC分类号： C12N15/53 ,  C12N1/21 ,  C12N9/04 ,  C12N15/63 ,  C12P7/18 ,  C12P41/00

CPC分类号： C12P7/18 ,  C12N9/0006

摘要： There is provided a polypeptide having the following physical and chemical properties: (1) potency: stereoselectively reducing 1-chloro-3-hydroxyacetone with the use of NADH as a coenzyme to thereby produce (S)-3-chloro-1,2-propanediol; (2) molecular weight: about 340,000 as measured by gel filtration analysis and about 43,000 as measured by SDS polyacrylamide gel electrophoresis analysis; (3) optimum temperature: 60˚ to 70˚C; (4) reduction optimum pH: 6.0, and (5) oxidation optimum pH: 9.0. Further, there are provided a polypeptide composed of an amino acid sequence of SEQ ID NO: 1 of the sequence listing; a DNA coding for the polypeptide; and a transformant capable of producing the polypeptide in high rate. Still further, there is provided a process for producing (S)-3-chloro-1,2-propanediol useful as a starting material of medicine, etc. in which use is made of the above polypeptide or transformant.