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1.发明公开N-ACETYLGLUCOSAMINE 2-EPIMERASE AND DNA ENCODING THE ENZYME 审中-公开N-ACETYLGLUCOSAMIN 2-EPIMERASE UND FUR DIESES ENZYM KODIERNDE DNA
公开(公告)号：EP1154018A1
公开(公告)日：2001-11-14
申请号：EP00902868.9
申请日：2000-02-09
发明人： KOIZUMI, Satoshi , TABATA, Kazuhiko , ENDO, Tetsuo , OZAKI, Akio
CPC分类号： C12P19/26 , C07K14/195 , C12N9/90
摘要： The present invention provides a novel protein having N -acetylglucosamine 2-epimerase activity; a DNA encoding the protein; a recombinant vector containing the DNA; a transformant obtainable by introducing the recombinant vector into a host cell; and a process for producing the protein or N -acetylmannosamine using the transformant.
摘要翻译： 本发明提供了具有N-乙酰葡糖胺2-差向异构酶活性的新型蛋白质; 编码蛋白质的DNA; 含有DNA的重组载体; 通过将重组载体导入宿主细胞获得的转化体; 以及使用该转化体生产蛋白质或N-乙酰甘露糖胺的方法。
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公开(公告)号：EP0861902A1
公开(公告)日：1998-09-02
申请号：EP97940364.9
申请日：1997-09-12
发明人： KOIZUMI, Satoshi , KAWANO, Hisaji , KINO, Kuniki , OZAKI, Akio
CPC分类号： C12P19/305 , C12N9/1051 , C12P19/02 , C12P19/18 , C12P19/26 , C12P19/30
摘要： This invention relates to a process for producing a complex carbohydrate, which comprises: selecting, as enzyme sources, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culture broth, and a culture broth of a microorganism or animal cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth; carrying out an enzyme reaction in an aqueous medium containing the enzyme sources, the nucleotide precursor, the sugar and the complex carbohydrate precursor to form and accumulate the complex carbohydrate in the aqueous medium; and recovering the complex carbohydrate from the aqueous medium, and a process for producing a sugar nucleotide, which comprises selecting, as an enzyme source, a culture broth of a microorganism capable of producing a sugar nucleotide from a nucleotide precursor and a sugar, or a treated product of the culture broth; carrying out an enzyme reaction in an aqueous medium containing the enzyme source, the nucleotide precursor and the sugar to form and accumulate the sugar nucleotide in the aqueous medium; and recovering the sugar nucleotide from the aqueous medium.
摘要翻译： 本发明涉及一种用于生产复合糖类，其包括：选择作为酶源，微生物的培养液中能够产生从核苷酸前体物质和糖，或该培养液的处理产物糖核苷酸的， 和能够产生从糖核苷酸和复合糖类前体，或该培养液的处理产物的复合碳水化合物的微生物或动物细胞的培养液; 在wässrige介质中进行酶反应在含有酶源，核苷酸前体物质，糖以及复合糖类前体以形成和积累在wässrige介质复合糖类; 并从该wässrige介质复合糖类，以及一种用于制造糖核苷酸的方法，包括选择，如在酶源，能够产生从核苷酸前体物质和糖，或糖核苷酸能力的微生物的培养液的处理 该培养液的处理产物; 进行在含有该酶源，核苷酸前体物质和糖以形成并积聚在wässrige介质中的糖核苷酸wässrige介质酶反应; 并从该介质wässrige糖核苷酸。
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公开(公告)号：EP1054062A1
公开(公告)日：2000-11-22
申请号：EP99901185.1
申请日：1999-01-27
发明人： KOIZUMI, Satoshi , ENDO, Tetsuo , TABATA, Kazuhiko , OZAKI, Akio
CPC分类号： C12N9/1051 , C12N9/1048 , C12P19/18 , Y10S435/822
摘要： The present invention provides a protein having β 1,4-galactosyltransferase activity, DNA encoding the protein, a recombinant DNA comprising the DNA, a transformant carrying the recombinant DNA, a process for producing β1,4-galactosyltransferase by using the transformant, and a process for producing a galactose-containing carbohydrate by using the transformant.
摘要翻译： 本发明提供具有β1,4-半乳糖基转移酶活性的蛋白质，编码该蛋白质的DNA，包含DNA的重组DNA，携带重组DNA的转化体，使用该转化体产生β1,4-半乳糖基转移酶的方法，以及 通过使用转化体生产含半乳糖的碳水化合物的方法。
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公开(公告)号：EP0870841A1
公开(公告)日：1998-10-14
申请号：EP97940365.6
申请日：1997-09-12
CPC分类号： C12P19/30
摘要： This invention relates to a process for producing a sugar nucleotide, in which a) a culture broth of a microorganism capable of producing NTP from a nucleotide precursor, or a treated product of the culture broth, and b) a culture broth of a microorganism capable of producing a sugar nucleotide from a sugar and NTP, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which the above-described a) and b) and c) a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, are used as enzyme sources; a process for producing a complex carbohydrate, in which a culture broth of a microorganism, an animal cell or an insect cell capable of producing a complex carbohydrate from a sugar nucleotide and a complex carbohydrate precursor, or a treated product of the culture broth, is as an enzyme source; and a process for producing N-acetylglucosamine-1-phosphate, in which a culture broth of a microorganism having strong galactokinase activity, or a treated product of the culture broth, is used as the enzyme source.
摘要翻译： 本发明涉及一种生产糖核苷酸的方法，其中a）能从核苷酸前体产生NTP的微生物的培养液或培养液的处理产物，和b） 从糖和NTP产生糖核苷酸或者培养肉汤的处理产物用作酶来源; 一种生产复合碳水化合物的方法，其中上述a）和b）和c）微生物的培养液，动物细胞或能从糖核苷酸生成复合碳水化合物的昆虫细胞和复合碳水化合物 前体或培养肉汤的处理过的产物用作酶源; 生产复合碳水化合物的方法，其中能从糖核苷酸和复合碳水化合物前体产生复合碳水化合物的微生物，动物细胞或昆虫细胞的培养液或培养液的处理产物是 作为酶源; 和生产N-乙酰葡糖胺-1-磷酸的方法，其中使用具有强半乳糖激酶活性的微生物的培养液或培养液的处理产物作为酶源。
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