PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS
    10.
    发明公开
    PREPARATION METHOD OF LACTATE POLYMERS AND LACTATE COPOLYMERS USING POLYHYDROXYALKANOATE SYNTHASE MUTANTS 审中-公开
    聚羟基乙酸酯合成酶突变体制备乳酸聚合物和乳酸盐共聚物的方法

    公开(公告)号:EP2471910A2

    公开(公告)日:2012-07-04

    申请号:EP10794355.7

    申请日:2010-06-30

    IPC分类号: C12N9/00 C12N15/52 C12P7/56

    摘要: Mutants of various polyhydroxyalkanoate (PHA) synthases capable of synthesizing a lactate polymer (PLA) and a lactate copolymer (PLA copolymer), and a method of preparing a lactate polymer and a lactate copolymer using the same are provided. More specifically, a mutant of polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8, and a method of preparing lactate polymer and lactate copolymer using the mutant of synthase are provided. The polyhydroxyalkanoate synthase set forth in SEQ ID NO: 2, 4, 6, or 8 can have an activity of synthesizing a lactate polymer and a lactate copolymer by an amino acid sequence mutation affecting an activity of synthesizing a lactate polymer, and can produce a lactate polymer and a copolymer that have different features, respectively, by using the mutants of the synthase.

    摘要翻译: 提供了能够合成乳酸酯聚合物(PLA)和乳酸酯共聚物(PLA共聚物)的各种聚羟基链烷酸酯(PHA)合酶的突变体,以及使用该突变体制备乳酸酯聚合物和乳酸酯共聚物的方法。 更具体而言,提供了SEQ ID NO:2,4,6或8中所示的聚羟基链烷酸酯合酶的突变体,以及使用该合酶突变体来制备乳酸盐聚合物和乳酸盐共聚物的方法。 在SEQ ID NO:2,4,6或8中列出的聚羟基链烷酸酯合酶可以具有通过影响合成乳酸聚合物活性的氨基酸序列突变合成乳酸酯聚合物和乳酸酯共聚物的活性,并且可以产生 乳酸聚合物和具有不同特征的共聚物,分别通过使用合酶的突变体。