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公开(公告)号：EP0638087A4
公开(公告)日：1994-12-27
申请号：EP93907533
申请日：1993-03-17
摘要： The present invention provides novel modified nucleotide compounds having the general formula: X-n(J)-(d or r)NTP where N is adenosine, guanosine or cytidine; X is H, a fluorophore, a chromophore, a luminescent compound, a ligand or a hapten; n is an integer of 7 or more; and J is a Jeffamine (TM Texaco, Inc.) substituent. These compounds are substantially superior substrates for incorporating label into nucleic acids during enzyme-catalyzed synthesis. Use of a Jeffamine-linked labelled nucleotide derivative as a partial substitute for unlabelled substrate therefore leads to significantly greater incorporation, hence increased label density per unit length of nucleic acid, than the same label joined by a prior art linker. Jeffamine-modified ribo- or deoxyribo-nucleotides are also provided for synthesis of Jeffamine-modified nucleic acids. The invention further provides an improved method of synthesizing labelled nucleic acids. This method provides greater frequency and higher efficiency of label incorporation, thus requiring lower amounts of nucleotide derivative in the reaction mixture.
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2.发明公开
公开(公告)号：EP0942917A4
公开(公告)日：2006-01-18
申请号：EP97938279
申请日：1997-08-14
发明人： RASHTCHIAN AYOUB , SOLUS JOSEPH
CPC分类号： C12N9/1252 , C12N9/96 , C12P19/34 , C12Q1/686 , C12Q1/6869 , C12Q2527/149 , C12Q2527/137 , C12Q2527/125
摘要： This invention is directed to compositions comprising mixtures of reagents, including thermostable enzymes (e.g., thermostable DNA polymerases), buffers, cofactors and other components, suitable for immediate use in nucleic acid amplification or sequencing techniques without dilution or addition of further components. The compositions contain no stabilizing agents (e.g., glycerol or serum albumin) and unexpectedly maintain activity for extended periods of time upon storage at temperature above freezing. These compositions are useful, alone or in the form of kits, for nucleic acid amplification (e.g., by the Polymerase Chain Reaction, as exemplified in the Figure) and sequencing (e.g., by dideoxy or 'Sanger' sequencing), or for any procedure utilizing thermostable DNA polymerases in a variety of medical, forensic and agricultural applications. In particular, the compositions and methods are useful for amplifying and sequencing nucleic acid molecules that are larger than about 7 kilobases in size.
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3.发明公开COMPOSITIONS AND METHODS FOR REVERSE TRANSCRIPTASE-POLYMERASE CHAIN REACTION (RT-PCR) 失效ZUSAMMENSETZUNGEN UND VERFAHREN DER REVERSEN TRANSKRIPTASE-POLYMERASEKETTENREAKTION（RT-PCR）
公开(公告)号：EP0975806A4
公开(公告)日：2004-10-06
申请号：EP98917984
申请日：1998-04-03
发明人： LEE JUN E , RASHTCHIAN AYOUB
CPC分类号： C12P19/34 , C12N9/1276 , C12N15/1096 , C12Q1/6846 , C12Q2527/149 , C12Q2527/125 , C12Q2521/107
摘要： The present invention is directed to compositions and methods useful for the amplification of nucleic acid molecules by reverse transcriptase-polymerase chain reaction (RT-PCR). Specifically, the invention provides compositions and methods for the amplification of nucleic acid molecules in a simplified one- or two-step RT-PCR procedure using combinations of reverse transcriptase and thermostable DNA polymerase enzymes in conjunction with sulphur-containing molecules or acetate-containing molecules (or combinations of such sulphur-containing molecules and acetate-containing molecules), and optionally bovine serum albumin. The invention thus facilitates the rapid and efficient amplification of nucleic acid molecules and the detection and quantitation of RNA molecules. The invention also is useful in the rapid production and amplification of cDNAs which may be used for a variety of industrial, medical and forensic purposes.
摘要翻译： 本发明涉及通过逆转录酶 - 聚合酶链反应（RT-PCR）扩增核酸分子的组合物和方法。 具体地，本发明提供了使用逆转录酶和耐热DNA聚合酶与含硫分子或含乙酸酯分子结合的简化的一步或两步RT-PCR方法扩增核酸分子的组合物和方法 （或这种含硫分子和含乙酸酯的分子的组合）和任选的牛血清白蛋白。 因此，本发明促进了核酸分子的快速和有效的扩增以及RNA分子的检测和定量。 本发明也可用于快速生产和扩增可用于各种工业，医学和法医目的的cDNA。
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