公开(公告)号：EP1565557A2

公开(公告)日：2005-08-24

申请号：EP03706015.9

申请日：2003-01-31

申请人： Large Scale Biology Corporation

发明人： PADGETT, Hal, S. ,  VAEWHONGS, Andrew, A. ,  VOJDANI, Fakhrieh, S. ,  SMITH, Mark, L. ,  LINDBO, John, A. ,  FITZMAURICE, Wayne, P.

IPC分类号： C12N15/09

CPC分类号： C12N15/102 ,  C07K14/005 ,  C12N9/22 ,  C12N15/1027 ,  C12N15/8203 ,  C12N15/8257 ,  C12N2770/00022

摘要： We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.

摘要翻译： 我们在这里描述限制性内切酶及其用途。 限制性核酸内切酶可用于发现单核苷酸多态性。 它们也可用于重新分配不相同多核苷酸序列之间的序列变异的体外方法。

公开(公告)号：EP1934335A2

公开(公告)日：2008-06-25

申请号：EP06836129.4

申请日：2006-09-08

申请人： Large Scale Biology Corporation

发明人： LINDBO, John, A. ,  SMITH, Mark, L. ,  PALMER, Kenneth, E.

IPC分类号： C12N7/01

CPC分类号： C07K14/005 ,  A61K39/12 ,  A61K39/21 ,  A61K39/385 ,  A61K2039/5258 ,  A61K2039/55566 ,  A61K2039/6075 ,  A61K2039/625 ,  C07K2319/00 ,  C07K2319/22 ,  C07K2319/60 ,  C12N7/00 ,  C12N15/8203 ,  C12N15/8257 ,  C12N15/8258 ,  C12N2710/20022 ,  C12N2710/20023 ,  C12N2710/20034 ,  C12N2740/16122 ,  C12N2740/16134 ,  C12N2770/00022

摘要： Compositions and methods to utilize coat proteins of tobacco mosaic virus (TMV) as a scaffold with commonly available linker chemistries and surface antigens to enhance immune responses when used as a vaccination; randomized library to introduce a reactive lysine or cysteine residue externally located at the amino-terminus of the coat protein. TMV coat protein modification allows a controlled stoichiometric biotinylation of the Introduced lysine or cysteine residues further allowing the attachment of antigens using streptavidin as a linker to the biotinylated coat protein. A TMV-protein complex vaccine prepared utilizing this invention results in enhanced immunogenicity and a detectable humoral immune response after only a single immunization in mice.

公开(公告)号：EP1565557B1

公开(公告)日：2007-07-11

申请号：EP03706015.9

申请日：2003-01-31

申请人： Large Scale Biology Corporation

发明人： PADGETT, Hal, S. ,  VAEWHONGS, Andrew, A. ,  VOJDANI, Fakhrieh, S. ,  SMITH, Mark, L. ,  LINDBO, John, A. ,  FITZMAURICE, Wayne, P.

IPC分类号： C12N15/10 ,  C12N15/55 ,  C12N9/22

CPC分类号： C12N15/102 ,  C07K14/005 ,  C12N9/22 ,  C12N15/1027 ,  C12N15/8203 ,  C12N15/8257 ,  C12N2770/00022

摘要： We describe here restriction endonucleases and their uses. Restriction endonucleases are useful in finding single nucleotide polymorphisms. They are also useful in an in vitro method of redistributing sequence variations between non-identical polynucleotide sequences.

公开(公告)号：EP1461620A2

公开(公告)日：2004-09-29

申请号：EP02798479.8

申请日：2002-12-04

申请人： Large Scale Biology Corporation

发明人： SMITH, Mark, L. ,  PALMER, Kenneth ,  POGUE, Gregory, P. ,  LINDBO, John, A. ,  HANLEY, Kathleen, M. ,  MANNION, David. P. ,  WOLFE, Gershon, M.

IPC分类号： G01N33/554

CPC分类号： C07K5/06165 ,  A61K38/00 ,  B01D15/1885 ,  B01D15/3804 ,  C07K5/06139

摘要： A plurality of proteins of interest, or peptides of interest, or other genetically expressed materials, are screened and subsequently produced using any of a variety of expression systems. The plurality of proteins are extracted from a plurality of separate, processed green juices, each green juice containing one of the proteins of interest. A multi-channel apparatus processes the various green juices, one green juice per channel. The apparatus is computer controlled such that the various valves in each channel and pump are controlled in an automated manner to extract each protein of interest and deliver each protein of interest into its own storage vessel.