公开(公告)号：EP3546471A1

公开(公告)日：2019-10-02

申请号：EP17874515.4

申请日：2017-10-12

申请人： NEC Solution Innovators, Ltd. ,  National University Corporation Gunma University

发明人： MINAGAWA Hirotaka ,  HORII Katsunori ,  AKITOMI Jou ,  KANEKO Naoto ,  WAGA Iwao ,  KUWAHARA Masayasu

IPC分类号： C07H19/073 ,  C12N15/09

摘要： The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1).

In the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, L 1 and L 2 are each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, X 1 is an imino group (-NR 1 -), an ether group (-O-), or a thioether group (-S-), and R 1 is a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

公开(公告)号：EP3514164B1

公开(公告)日：2020-09-09

申请号：EP17850964.2

申请日：2017-09-14

申请人： NEC Solution Innovators, Ltd. ,  National University Corporation Gunma University

发明人： MINAGAWA Hirotaka ,  HORII Katsunori ,  AKITOMI Jou ,  KANEKO Naoto ,  WAGA Iwao ,  KUWAHARA Masayasu

IPC分类号： C07H19/073 ,  C07H19/10 ,  C07H21/00 ,  C12N9/10 ,  C12N15/115

公开(公告)号：EP3514165A1

公开(公告)日：2019-07-24

申请号：EP17850965.9

申请日：2017-09-14

申请人： NEC Solution Innovators, Ltd. ,  National University Corporation Gunma University

发明人： MINAGAWA Hirotaka ,  HORII Katsunori ,  AKITOMI Jou ,  KANEKO Naoto ,  WAGA Iwao ,  KUWAHARA Masayasu

IPC分类号： C07H19/20 ,  C07K16/00 ,  C12N9/26 ,  C12N15/115

摘要： The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule. The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1):

where in the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, L 1 and L 2 are each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, X 1 and X 2 are each independently an imino group (-NR 1 -), an ether group (-O-), or a thioether group (-S-), and the R 1 is a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

公开(公告)号：EP3514164A1

公开(公告)日：2019-07-24

申请号：EP17850964.2

申请日：2017-09-14

申请人： NEC Solution Innovators, Ltd. ,  National University Corporation Gunma University

发明人： MINAGAWA Hirotaka ,  HORII Katsunori ,  AKITOMI Jou ,  KANEKO Naoto ,  WAGA Iwao ,  KUWAHARA Masayasu

IPC分类号： C07H19/073 ,  C07H19/10 ,  C07K16/00 ,  C12N9/26 ,  C12N15/115

摘要： The present invention provides a novel nucleoside derivative or a salt thereof, a polynucleotide synthesis reagent, a method for producing a polynucleotide, a polynucleotide, and a method for producing a binding nucleic acid molecule.
The nucleoside derivative or a salt thereof of the present invention is represented by the following chemical formula (1):

where in the chemical formula (1), Su is an atomic group having a sugar skeleton at a nucleoside residue or an atomic group having a sugar phosphate skeleton at a nucleotide residue, and may or may not have a protecting group, L 1 and L 2 are each independently a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms, X 1 and X 2 are each independently an imino group (-NR 1 -), an ether group (-O-), or a thioether group (-S-), and the R 1 is a hydrogen atom or a straight-chain or branched, saturated or unsaturated hydrocarbon group having 2 to 10 carbon atoms.

公开(公告)号：EP3406735A1

公开(公告)日：2018-11-28

申请号：EP17741546.0

申请日：2017-01-20

申请人： NEC Solution Innovators, Ltd.

发明人： YOSHIDA Yoshihito ,  HORII Katsunori ,  AKITOMI Jou ,  KANEKO Naoto ,  SHIMIZU Akihisa ,  FUJITA Tomoko ,  WAGA Iwao

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12N15/09 ,  G01N33/53 ,  G01N33/543 ,  C12N9/02

CPC分类号： C12M1/00 ,  C12N15/09 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/543

摘要： The present invention provides a new target analysis method and a target analysis kit for use in the method. The target analysis method of the present invention includes: causing a specimen, a labeled binding nucleic acid molecule that binds to a target, and a carrier on which a blocking nucleic acid molecule that binds to the labeled binding nucleic acid molecule is immobilized to react; separating a fraction of the carrier and a fraction of components other than the carrier from each other; and detecting a label of the labeled binding nucleic acid molecule in at least one of the fraction of the carrier and the fraction of components other than the carrier to analyze a target in the specimen.

公开(公告)号：EP3026114A1

公开(公告)日：2016-06-01

申请号：EP14829556.1

申请日：2014-06-27

申请人： NEC Solution Innovators, Ltd.

发明人： KANEKO Naoto ,  SHIRATORI Ikuo ,  HORII Katsunori ,  AKITOMI Jou ,  WAGA Iwao

IPC分类号： C12N15/115 ,  C12N15/09 ,  C12Q1/68 ,  G01N21/64 ,  G01N21/78 ,  G01N33/53 ,  G01N33/542

CPC分类号： G01N33/04 ,  C12N15/11 ,  C12N15/115 ,  C12N2310/127 ,  C12N2310/151 ,  C12N2310/16 ,  C12N2310/3519 ,  C12Q1/6825 ,  G01N33/542 ,  C12Q2521/345 ,  C12Q2563/107

摘要： The present invention is intended to provide a novel fluorescence sensor for target analysis, a kit for target analysis, and a target analysis method using the same.
The fluorescence sensor for target analysis according to the present invention includes a nucleic acid molecule that includes a G-quartet-forming nucleic acid region (D) that forms a G-quartet and a binding nucleic acid region (A) that binds to a target. In the absence of a target, formation of a G-quartet in the G-quartet-forming nucleic acid region (D) is inhibited. In the presence of a target, the target comes into contact with the binding nucleic acid region (A), the G-quartet is formed in the G-quartet-forming nucleic acid region (D) due to the contact, the G-quartet-forming region (D) and porphyrin forms a complex, and the complex generates fluorescence.

摘要翻译： 本发明旨在提供一种用于靶分析的新型荧光传感器，用于目标分析的试剂盒和使用其的目标分析方法。 根据本发明的用于靶分析的荧光传感器包括核酸分子，其包含形成G-四重态的G-四重峰形成核酸区（D）和与靶标结合的结合核酸区（A） 。 在不存在靶的情况下，G-四重峰形成核酸区（D）中的G四重奏的形成被抑制。 在靶的存在下，靶与结合核酸区（A）接触，G-四重峰由于接触而形成在G-四重峰形成核酸区（D）中，G-四重峰 形成区域（D）和卟啉形成复合物，并且复合物产生荧光。

公开(公告)号：EP3078747A1

公开(公告)日：2016-10-12

申请号：EP14867903.8

申请日：2014-06-26

申请人： NEC Solution Innovators, Ltd.

发明人： MURATA Tomoko ,  HORII Katsunori ,  SHIRATORI Ikuo ,  AKITOMI Jou ,  KANEKO Naoto ,  WAGA Iwao

IPC分类号： C12N15/115 ,  C12Q1/68 ,  G01N33/53

CPC分类号： C12N15/115 ,  C12N2310/16 ,  C12Q1/6895

摘要： The present invention provides a novel nucleic acid molecule that can be used for detection of peanuts. The peanut-binding nucleic acid molecule of the present invention is characterized in that it binds to a peanut allergen with a dissociation constant of 10 nM or less, and preferably contains a polynucleotide consisting of any of base sequences of SEQ ID NOs: 1 to 15, for example. It is also preferable that, for example, the peanut-binding nucleic acid molecule of the present invention binds with significant specificity to the peanut allergen rather than to a soybean protein.

摘要翻译： 本发明提供了可用于检测花生的新型核酸分子。 本发明的花生结合核酸分子的特征在于其以10nM以下的解离常数结合花生过敏原，优选含有由SEQ ID NO：1〜15的碱基序列中的任一种组成的多核苷酸 ， 例如。 还优选的是，本发明的花生结合核酸分子对花生过敏原而不是大豆蛋白具有显着特异性的结合。

公开(公告)号：EP2886664A1

公开(公告)日：2015-06-24

申请号：EP13822588.3

申请日：2013-07-23

申请人： NEC Solution Innovators, Ltd.

发明人： HORII Katsunori ,  KANEKO Naoto ,  AKITOMI Jou ,  KATOU Shintarou ,  FURUICHI Makio ,  WAGA Iwao

IPC分类号： C12Q1/68 ,  C12M1/00 ,  C12N11/00 ,  C12N15/115

CPC分类号： G01N33/5308 ,  C12N15/111 ,  C12N15/113 ,  C12N15/115 ,  C12N2310/127 ,  C12N2310/16 ,  C12N2310/3519 ,  C12N2320/10 ,  C12Q1/6825 ,  G01N21/251 ,  G01N21/76 ,  G01N33/581 ,  G01N2333/9005 ,  C12Q2521/345 ,  C12Q2525/205

摘要： The present invention is to provide a new sensor for melamine detection. The nucleic acid sensor for melamine analysis of the present invention includes a polynucleotide (xl) that includes a catalytic nucleic acid molecule (D) that activates a catalytic function and a binding nucleic acid molecule (A) that binds to melamine. The polynucleotide (xl) has any one of the base sequences of SEQ ID NOs: 1 to 14, and n and m are positive integers. In the nucleic acid sensor, since the catalytic function of the catalytic nucleic acid molecule (D) is inhibited in the absence of melamine and the catalytic function of the catalytic nucleic acid molecule (D) is activated in the presence of melamine, melamine can be analyzed by detecting the catalytic function.

摘要翻译： 本发明提供一种用于三聚氰胺检测的新型传感器。 用于本发明的三聚氰胺分析的核酸传感器包括包含活化催化功能的催化核酸分子（D）和结合三聚氰胺的结合核酸分子（A）的多核苷酸（x1）。 多核苷酸（x1）具有SEQ ID NO：1至14的任何一个碱基序列，n和m是正整数。 在核酸传感器中，由于在没有三聚氰胺的情况下催化核酸分子（D）的催化功能被抑制，并且催化性核酸分子（D）的催化功能在三聚氰胺的存在下被活化，所以三聚氰胺可以 通过检测催化功能进行分析。