摘要:
An object of the present invention is to provide a composition for producing a fiber, which is preferable for immobilizing substances effective for cell adhesion. proliferation.differentiation and the like, and retains organic solvent resistance, a fiber obtained by spinning the composition, and a cell culture scaffold material using the fiber. A composition for producing a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1) and a unit structure represented by the formula (2), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent
wherein each symbol in the formulas (1) and (2) is as described in the DESCRIPTION.
摘要:
The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.
摘要:
The present invention provides a culture method of cells and/or tissues including culturing cells and/or tissues in a suspended state by using a medium composition wherein indeterminate structures are formed in a liquid medium, the structures are uniformly dispersed in the solution and substantially retain the cells and/or tissues without substantially increasing the viscosity of the solution, thus affording an effect of preventing sedimentation thereof, and the like.
摘要:
The present invention provides a composition for forming a fiber for the production of a fiber having organic solvent resistance, which can be produced conveniently, a fiber obtained by spinning the composition, and a biocompatible material containing the fiber. A composition for forming a fiber, containing (A) a polymer compound containing a unit structure represented by the formula (1), (B) a crosslinking agent, (C) an acid compound, and (D) a solvent. wherein each symbol is as described in the DESCRIPTION.
摘要:
The present invention provides: a cell cultivation vessel characterized in that the surface is coated with a copolymer comprising repeating units having organic groups represented by formula (a) and repeating units having organic groups represented by formula (b); a manufacturing method therefor; and a cell aggregate-manufacturing method using same (in the formulas, U a1 , U a2 , U b1 , U b2 and U b3 as well as An - are as set forth in the Description and the Claims).
摘要:
The present invention provides a method of producing erythrocytes, including efficiently differentiating hematopoietic stem cells and/or a hematopoietic progenitor cells into erythrocytes by using a medium composition having an effect of homogeneously dispersing the hematopoietic stem cells and/or the hematopoietic progenitor cells and maintaining a floating state.
摘要:
The present invention provides a method of isolating cells or tissues from a culture preparation of the cells or tissues in a medium composition which enables culture of the cells or tissues in suspension, which comprises at least one step selected from the group consisting of the following (A), (B) and (C): (A) passing the culture preparation through a filter having fine pores having a pore diameter of 5 - 500 µm, (B) adding a chelator to the culture preparation, and (C) diluting the culture preparation with a physiological aqueous solution.
摘要:
An object to the present invention is to provide a fiber which is superior in safety, can be conveniently produced, and has organic solvent resistance, a starting material composition for producing the fiber and a biocompatible material containing the fiber. A fiber produced by spinning a composition containing (A) a condensation product obtained by condensing one or more kinds of a compound represented by the formula (1), and (B) an acid compound:
wherein each symbol is as defined in the DESCRIPTION.
摘要:
This is to provide a blood filter comprising a porous element; and a copolymer which exists in at least a part of a surface of the porous element, and which contains a recurring unit containing an organic group of the following formula (a) and a recurring unit containing an organic group of the following formula (b), wherein U a1 , U a2 , U b1 , U b2 and U b3 , and An - are as defined in the present specification and the claims.
摘要:
The present invention provides a medium additive, medium composition and a culture method and the like, capable of efficiently culturing cells or tissues in a well dispersed state, and further, permitting cell image analysis of the cells or tissues. The medium additive or medium composition of the present invention contains agar. As the agar, low molecular weight agar having a weight average molecular weight of 10,000 - 60,000 is preferably used. Using same, cells or tissues can be cultured in a well-dispersed state in a medium, and a proliferation promoting effect for the cells or tissues can also be obtained. In addition, the cells can be cultured in any of a floating state and a precipitated state by adjusting the concentration of the aforementioned agar.