公开(公告)号：EP1054994B1

公开(公告)日：2004-11-17

申请号：EP99911678.3

申请日：1999-02-17

申请人： Novartis AG ,  Novartis Pharma GmbH

发明人： HOFMANN, Hans ,  MAHNKE, Marion ,  MEMMERT, Klaus ,  PETERSEN, Frank ,  SCHUPP, Thomas ,  KÜSTERS, Ernst ,  MUTZ, Michael

IPC分类号： C12P17/18 ,  C07D493/04 ,  B01D15/08 ,  A61K31/335 ,  A61P35/00

CPC分类号： C07D493/04 ,  C07D417/14 ,  C07K2299/00 ,  C12P17/167 ,  C12P17/181 ,  C12R1/01

公开(公告)号：EP0929681B1

公开(公告)日：2006-09-13

申请号：EP97938923.6

申请日：1997-08-18

申请人： Novartis AG ,  Novartis Pharma GmbH

发明人： SCHUPP, Thomas ,  TOUPET, Christiane ,  ENGEL, Nathalie

IPC分类号： C12N15/52 ,  C12P17/18 ,  C12P17/10 ,  C12N9/00 ,  C12N1/21 ,  C12N15/70 ,  C12Q1/68

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P17/10 ,  C12P17/189

摘要： The present invention primarily relates to a DNA fragment which is obtainable from the gene cluster responsible for rifamycin biosynthesis within the genome of Amycolatopsis mediterranei, and comprises at least one gene or a part of a gene which codes for a polypeptide which is directly or indirectly involved in the biosynthesis of rifamycin, and to a method for preparing said DNA fragment. The present invention furthermore relates to recombinant DNA molecules which comprise one of the DNA fragments according to the invention, and to the plasmids and vectors derived therefrom. Host organisms transformed with said plasmid or vector DNA are likewise embraced.

公开(公告)号：EP1088078A2

公开(公告)日：2001-04-04

申请号：EP99929243.6

申请日：1999-06-16

申请人： Novartis AG ,  Novartis-Erfindungen Verwaltungsgesellschaft m.b.H.

发明人： SCHUPP, Thomas ,  LIGON, James, Madison ,  MOLNAR, Istvan ,  ZIRKLE, Ross ,  GÖRLACH, Jörn ,  CYR, Devon

IPC分类号： C12N15/52 ,  C07K14/535 ,  C07D493/00

CPC分类号： C12P17/181 ,  C07K14/195

摘要： Nucleic acid molecules are isolated from Sorangium cellulosum that encode polypeptides necessary for the biosynthesis of epothilone. Disclosed are methods for the production of epothilone in recombinant hosts transformed with the genes of the invention. In this manner, epothilone can be produced in quantities large enough to enable their purification and use in pharmaceutical formulations such as those for the treatment of cancer.

公开(公告)号：EP0929681A1

公开(公告)日：1999-07-21

申请号：EP97938923.0

申请日：1997-08-18

申请人： Novartis AG

发明人： SCHUPP, Thomas ,  TOUPET, Christiane ,  ENGEL, Nathalie

IPC分类号： C12N1 ,  C12N9 ,  C12N15 ,  C12P17 ,  C12Q1

CPC分类号： C12N9/93 ,  C12N15/52 ,  C12P17/10 ,  C12P17/189

摘要： The present invention primarily relates to a DNA fragment which is obtainable from the gene cluster responsible for rifamycin biosynthesis within the genome of Amycolatopsis mediterranei, and comprises at least one gene or a part of a gene which codes for a polypeptide which is directly or indirectly involved in the biosynthesis of rifamycin, and to a method for preparing said DNA fragment. The present invention furthermore relates to recombinant DNA molecules which comprise one of the DNA fragments according to the invention, and to the plasmids and vectors derived therefrom. Host organisms transformed with said plasmid or vector DNA are likewise embraced.

公开(公告)号：EP1054994A2

公开(公告)日：2000-11-29

申请号：EP99911678.3

申请日：1999-02-17

申请人： Novartis AG ,  Novartis-Erfindungen Verwaltungsgesellschaft m.b.H.

发明人： HOFMANN, Hans ,  MAHNKE, Marion ,  MEMMERT, Klaus ,  PETERSEN, Frank ,  SCHUPP, Thomas ,  KÜSTERS, Ernst ,  MUTZ, Michael

IPC分类号： C12P17/18 ,  C07D493/04 ,  B01D15/08

CPC分类号： C07D493/04 ,  C07D417/14 ,  C07K2299/00 ,  C12P17/167 ,  C12P17/181 ,  C12R1/01

摘要： The invention relates to a process for concentrating epothilones in culture media, a process for the production of epothilones, a process for separating epothilones A and B and a strain obtained by mutagenesis for the production of epothilones, as well as aspects related thereto. Crystal forms of epothilone B are also described.