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公开(公告)号：EP0183071A3
公开(公告)日：1987-09-23
申请号：EP85113737
申请日：1985-10-29
发明人： Stroman, David Womack , Brust, Paul Frederick , Ellis, Steven Bradley , Gingeras, Thomas Raymond , Harpold, Michael Miller , Tschopp, Juerg Friedrich
IPC分类号： C12N15/00 , C07H21/04 , C12N01/20 , C12N01/16 , C12R01/19 , C12R01/645 , C12R01/72 , C12R01/78 , C12R01/84 , C12R01/85 , C12R01/88
CPC分类号： C12N9/0006 , C12N15/52 , C12N15/68 , C12N15/70 , C12N15/815
摘要： Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.
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公开(公告)号：EP0226846B1
公开(公告)日：1993-08-04
申请号：EP86116302.0
申请日：1986-11-25
发明人： Tschopp, Juerg Friedrich , Harpold, Michael Miller , Cregg, James Michael , Buckholz, Richard Gordon
CPC分类号： C07K14/005 , C12N15/815 , C12N2730/10122
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公开(公告)号：EP0256421A2
公开(公告)日：1988-02-24
申请号：EP87111245.4
申请日：1987-08-04
CPC分类号： C12N9/2408 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/815 , C12N2770/36122
摘要： Method for production by yeast of secreted proteins having a pattern of glycosylation substantially similar to the high mannose-type mammalian protein pattern of glycosylation, and DNA sequences useful therefor are described. In addition, method for rendering yeast strains competent for growth on carbon and energy sources on which prototrophic strains of yeast are unable to grow is provided.
摘要翻译： 描述了具有基本上类似于糖基化的高甘露糖型哺乳动物蛋白质模式的糖基化模式的分泌蛋白质的酵母生产方法，以及对其有用的DNA序列。 另外，提供了能够在酵母生育菌株不能生长的碳源和能源上生长的酵母菌株的方法。
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公开(公告)号：EP0183071A2
公开(公告)日：1986-06-04
申请号：EP85113737.2
申请日：1985-10-29
发明人： Stroman, David Womack , Brust, Paul Frederick , Ellis, Steven Bradley , Gingeras, Thomas Raymond , Harpold, Michael Miller , Tschopp, Juerg Friedrich
CPC分类号： C12N9/0006 , C12N15/52 , C12N15/68 , C12N15/70 , C12N15/815
摘要： Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.
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公开(公告)号：EP0183071B1
公开(公告)日：1992-12-09
申请号：EP85113737.2
申请日：1985-10-29
发明人： Stroman, David Womack , Brust, Paul Frederick , Ellis, Steven Bradley , Gingeras, Thomas Raymond , Harpold, Michael Miller , Tschopp, Juerg Friedrich
CPC分类号： C12N9/0006 , C12N15/52 , C12N15/68 , C12N15/70 , C12N15/815
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公开(公告)号：EP0256421A3
公开(公告)日：1989-04-05
申请号：EP87111245.4
申请日：1987-08-04
CPC分类号： C12N9/2408 , C07K14/005 , C07K2319/00 , C07K2319/02 , C12N15/815 , C12N2770/36122
摘要： Method for production by yeast of secreted proteins having a pattern of glycosylation substantially similar to the high mannose-type mammalian protein pattern of glycosylation, and DNA sequences useful therefor are described. In addition, method for rendering yeast strains competent for growth on carbon and energy sources on which prototrophic strains of yeast are unable to grow is provided.
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公开(公告)号：EP0226846A1
公开(公告)日：1987-07-01
申请号：EP86116302.0
申请日：1986-11-25
发明人： Tschopp, Juerg Friedrich , Harpold, Michael Miller , Cregg, James Michael , Buckholz, Richard Gordon
CPC分类号： C07K14/005 , C12N15/815 , C12N2730/10122
摘要： Novel DNA constructs comprising regulatory regions plus the structural coding region for hepatitis B surface antigen (HBsAg) are disclosed. The regulatory regions employed are responsive to methanol, non-catabolite repressing carbon sources and catabolite repressing carbon sources followed by carbon source starvation. The novel constructs are incorporated into a variety of linear and circular plasmids. Such plasmids are used to transform suitable hosts and ultimately used for the production and isolation of hepatitis B surface antigen in high yields.
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