公开(公告)号：EP0183071A3

公开(公告)日：1987-09-23

申请号：EP85113737

申请日：1985-10-29

申请人： PHILLIPS PETROLEUM COMPANY

发明人： Stroman, David Womack ,  Brust, Paul Frederick ,  Ellis, Steven Bradley ,  Gingeras, Thomas Raymond ,  Harpold, Michael Miller ,  Tschopp, Juerg Friedrich

IPC分类号： C12N15/00 ,  C07H21/04 ,  C12N01/20 ,  C12N01/16 ,  C12R01/19 ,  C12R01/645 ,  C12R01/72 ,  C12R01/78 ,  C12R01/84 ,  C12R01/85 ,  C12R01/88

CPC分类号： C12N9/0006 ,  C12N15/52 ,  C12N15/68 ,  C12N15/70 ,  C12N15/815

摘要： Novel DNA sequences which are responsive to the presence of methanol, catabolite non-repressing carbon sources and carbon source starvation are provided. In addition, novel constructs including these DNA sequences, as well as transformed organisms therewith are provided. Processes for producing the DNA sequences and constructs of the invention are detailed. The production of polypeptide product under the control of the regulatory regions of the invention is demonstrated.

公开(公告)号：EP0139114A3

公开(公告)日：1986-05-28

申请号：EP84108823

申请日：1984-07-25

申请人： LABATT BREWING COMPANY LIMITED

发明人： Stewart, Graham George ,  Russell, Ingeborg ,  Panchal, Chandrakant Jagjivan

IPC分类号： C12N01/16 ,  C12P07/06 ,  C12N15/00 ,  C12R01/85

CPC分类号： C12R1/85 ,  C12N15/04 ,  C12P7/06 ,  Y02E50/17

摘要： The present invention relates to a stable, allopolyploid, somatic fusion product useful in the fermentation arts and especially in the production of fuel alcohols. More particularly, the present invention relates to a novel allopolyploid yeast strain, Saccharomyces diastaticus NCYC 1460, which is the product of the spheroplast fusion of:
(a) a hybrid, diploid strain of Sacch.diastaticus; and (b) a fusion partner which is a polyploid brewing yeast strain of the species Saccharomyces uvarum (carlsber- gensisJ. The fusion product of the present invention is capable of an enhanced rate of fermentation and a generally higher degree of final attenuation than is typical of either of the fusion partners. Moreover the fusion product also evidences enhanced osmo- and thermo-tolerances and its multiple, non-allelic DEX gene complement renders the organism well suited to the fermentation of substrates containing dextrins and oligosaccharides.

公开(公告)号：EP0170243A3

公开(公告)日：1987-08-26

申请号：EP85109501

申请日：1985-07-29

申请人： INTERNATIONAL FLAVORS & FRAGRANCES INC.

发明人： Farbood, Mohamad Ibrahim ,  Morris, James A. ,  Seitz, Eugene W.

IPC分类号： C12P07/62 ,  C12R01/645 ,  C12R01/72 ,  C12R01/78 ,  C12R01/84 ,  C12R01/85

CPC分类号： C12P7/62

摘要： Esters having useful organo liptic properties and having the generic structure:
wherein R 1 , R 2 and R 3 each represents methyl or hydrogen; wherein the dashed line represents a carbon-carbon single bond or a carbon-carbon double bond wherein R' represents C 2 -C 5 alkyl may be obtained by reacting an amino acid having the structure:
with a C 2 -C 5 alkanol in the presence of a fermentation organism capable of producing at least one of said esters.

公开(公告)号：EP0142790A3

公开(公告)日：1987-05-13

申请号：EP84113524

申请日：1984-11-09

申请人： Tanaka, Kentaro

发明人： Tanaka, Kentaro

IPC分类号： C12P09/00 ,  A61K33/26 ,  C12R01/645 ,  C12R01/85 ,  C12R01/865

CPC分类号： C12R1/645 ,  A61K33/26 ,  C12P9/00 ,  C12R1/85 ,  C12R1/865

摘要： An improved process for producing an organoiron (II) compound-containing antianaemic composition which comprises subjecting a yeast to anaerobic culturing in a saccharide-containing nutrient medium therefor in the presence of an iron compound. By the process, an antianaemic composition which is very stable and excellent in absorbabil- i ty into a living body and incorporation of iron into hemoglobin can be produced in high yield in a shortened period of time.

摘要翻译： 一种用于生产含有机铁（II）化合物的抗风湿性组合物的改进方法，其包括在铁化合物存在下，使酵母在含糖的营养培养基中进行厌氧培养。 通过该方法，可以在缩短的时间内以高产率制备非常稳定且对生物体的吸收性优异并且将铁掺入血红蛋白的抗风湿性组合物。

公开(公告)号：EP0096491A3

公开(公告)日：1986-02-19

申请号：EP83302879

申请日：1983-05-19

申请人： THE REGENTS OF THE UNIVERSITY OF CALIFORNIA

发明人： Fogel, Seymour ,  Welch, Juliet W. ,  Karin, Michael

IPC分类号： C12N15/00 ,  C12P21/02 ,  C07C103/52 ,  C07H21/04 ,  C12N01/16 ,  C12R01/85

CPC分类号： C12N15/67 ,  C07K14/825 ,  C12N15/81

摘要： Yeast metallothionein (copper chelatin) and DNA sequences having the gene encoding the polypeptide are provided. The DNA sequences find use in producing copper chelatin and in amplifying downstream flanking regions.

公开(公告)号：EP0126206A3

公开(公告)日：1985-03-06

申请号：EP84100834

申请日：1984-01-26

申请人： CETUS CORPORATION

发明人： Nunberg, Jack H. ,  Flatgaard, Jeffrey E. ,  Innis, Michael Alan ,  Gelfand, David Harrow ,  Meade, James Henry

IPC分类号： C12N15/00 ,  C12P21/02 ,  C07C103/52 ,  C07H21/04 ,  C12P07/06 ,  C12N09/34 ,  C12N01/16 ,  C12R01/85

CPC分类号： C12N9/2428 ,  C07K2319/02 ,  C07K2319/036 ,  C12N15/10 ,  C12N15/70 ,  C12N15/81 ,  C12P7/06 ,  Y02E50/17

摘要： A gene having a DNA sequence complementary to that of the glucoamylase polypeptide mRNA from a fungal species, preferably Aspergillus awamori, is prepared. The mRNA is an approximately 2.2 kilobase poly a RNA obtained from fungal cells grown under conditions of glucoamylase induction. Reverse transcription of the mRNA provides a glucoamylase probe used to identify genomic digest fragments containing glucoamylase gene regions, which are sequenced to locate the introns and exons. The genomic fragments are spliced together to form a gene having a DNA sequence with altered or deleted introns which codes for fungal glucoamylase protein and is capable, when correctly combined with a cleaved DNA expression vector, of expressing a non-native protein having glucoamylase enzyme activity upon transformation of a host organism by the vector. The host organism is preferably a bacterium or a yeast. The transformed host organism may be used to produce ethanol.

摘要翻译： 制备具有与来自真菌物种，优选泡盛曲霉的葡糖淀粉酶多肽mRNA互补的DNA序列的基因。 mRNA是从在葡糖淀粉酶诱导条件下生长的真菌细胞获得的约2.2千碱基多聚RNA。 mRNA的逆转录提供了用于鉴定含有葡糖淀粉酶基因区域的基因组消化片段的葡糖淀粉酶探针，其被测序以定位内含子和外显子。 将基因组片段拼接在一起以形成具有编码真菌葡糖淀粉酶蛋白并具有改变或缺失的内含子的DNA序列的基因，并且当其与切割的DNA表达载体正确结合时能够表达具有葡糖淀粉酶酶活性的非天然蛋白 通过载体转化宿主生物体。 宿主生物优选为细菌或酵母。 转化的宿主生物体可用于生产乙醇。