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    Vibrio cholerae lipoprotein 15 (Lp15) variants as anti-interference additive in TpN17-based immunoassays for detection of anti-Treponema antibodies
    2.
    发明公开
    Vibrio cholerae lipoprotein 15 (Lp15) variants as anti-interference additive in TpN17-based immunoassays for detection of anti-Treponema antibodies 有权
    霍乱弧菌脂蛋白15（LP15）变体作为抗干扰在添加剂的tpn17基于免疫测定用于检测抗密螺旋体抗体

    公开(公告)号：EP2827147A1

    公开(公告)日：2015-01-21

    申请号：EP14177199.8

    申请日：2014-07-16

    申请人： Roche Diagnostics GmbH ,  F. Hoffmann-La Roche AG

    发明人： Faatz, Elke ,  Schaarschmidt, Peter ,  Schmitt, Urban ,  Scholz, Christian

    IPC分类号： G01N33/53 ,  G01N33/571

    CPC分类号： G01N33/6854 ,  C07K14/245 ,  C07K14/28 ,  C07K2319/35 ,  G01N33/5306 ,  G01N33/571 ,  G01N2333/20 ,  G01N2333/28 ,  G01N2469/20 ,  G01N2800/26

    摘要： The invention relates to a method for detecting antibodies against the TpN17 antigen of Treponema pallidum in an isolated sample wherein a peptide sequence of Vibrio cholerae lipoprotein 15 ( Vc Lp15) or a partial sequence thereof is used as a reagent for reduction of interference , i.e. for minimizing false positive results. In addition the invention relates to fusion polypeptides comprising a Vc Lp15 peptide sequence and a chaperone, to their use as an additive in an immunoassay for said reduction of interferences and for minimizing false positive results and to a reagent kit for detecting antibodies against Treponema pallidum antigens in an isolated sample comprising a TpN17 antigen and said Vc Lp15-chaperone fusion polypeptide.

    摘要翻译： 本发明涉及用于检测抗体针对梅毒螺旋的的tpn17抗原的方法螺旋体在分离的样品worin霍乱弧菌脂蛋白15（VC LP15）或其部分序列组成的肽序列被用作试剂用于减少干扰的，即 为最大限度地减少假阳性结果。 此外，本发明涉及融合多肽，其包含一个VC LP15肽序列和分子伴侣，其使用如在免疫测定用于所述减少干扰的在添加剂的和用于最小化假阳性结果，并用试剂盒，用于检测抗体的抗梅毒螺旋体抗原 在分离的样品，其包括的tpn17抗原和Said Vc的LP15伴侣蛋白融合多肽。