METHODS FOR DNA AMPLIFICATION AND KITS THEREFOR
    5.
    发明授权
    METHODS FOR DNA AMPLIFICATION AND KITS THEREFOR 有权
    对于DNS方法被复制和材料THEREFOR

    公开(公告)号:EP1004676B1

    公开(公告)日:2006-03-15

    申请号:EP98936732.1

    申请日:1998-08-10

    申请人: TAKARA BIO INC.

    IPC分类号: C12Q1/68

    摘要: Methods for DNA amplification wherein a DNA is amplified by using a DNA fragment having nucleotide analogs as a template in the presence of the nucleotide analogs, characterized in that the amplification is effected in the presence of nucleotide analogs of two or more types and/or a compound capable of lowering the Tm value of a double-stranded nucleic acid; and kits for amplifying a DNA by using a DNA fragment having nucleotide analogs as a template in the presence of the nucleotide analogs, characterized by containing nucleotide analogs of two or more types and/or a compound capable of lowering the Tm value of a double-stranded nucleic acid. According to these methods, DNA fragments originating in RNAs can be amplified without preliminarily purifying the RNAs in samples, which contributes to the simplification of the experimental procedures and improvement in the reproducibility.