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公开(公告)号:EP2468850A1
公开(公告)日:2012-06-27
申请号:EP10809959.9
申请日:2010-08-17
申请人: Takara Bio, Inc.
发明人: TANABE, Masashige , ISONO, Tomomi , ENOKI, Tatsuji
CPC分类号: A01N1/0221
摘要: A method for cryopreservation of cells characterized by the use of a solution containing, as essential components, a sodium salt, a potassium salt, a saccharide, a cryoprotectant, and a hydrogencarbonate salt and/or a carbonate salt, the solution further optionally containing a component selected from the group consisting of proteins, magnesium salts, and calcium salts. According to the present invention, a method for cryopreservation of cells capable of maintaining a high viability rate after thawing, and a cryopreservation solution used in the cryopreservation are provided. In addition, by using the method for cryopreservation, the cells can be preserved in a state of maintaining a high viability rate even after thawing, and also maintaining their physiological functions; further, a cell washing step after freezing and thawing is unnecessary, thereby making it very useful in the fundamental researches of cells and the application studies on the medical treatment.
摘要翻译: 一种用于冷冻保存细胞的方法,其特征在于使用含有钠盐,钾盐,糖类,冷冻保护剂和碳酸氢盐和/或碳酸盐作为必要组分的溶液,该溶液还任选地含有 选自蛋白质,镁盐和钙盐的组分。 根据本发明,提供了一种在解冻后能够保持高活力率的细胞的低温保存方法和用于冷冻保存的冷冻保存溶液。 另外,通过使用冷冻保存方法,即使在解冻后也可以将细胞保持在维持高生存率的状态,并且还保持其生理功能; 此外,不需要冷冻和解冻后的细胞洗涤步骤,从而使其在细胞的基础研究和医疗应用研究中非常有用。
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公开(公告)号:EP1627632A1
公开(公告)日:2006-02-22
申请号:EP04733687.0
申请日:2004-05-18
申请人: TAKARA BIO INC.
发明人: ENOKI, Tatsuji , KOBAYASHI, Eiji , OGAWA, Kinuko , KUDO, Yoko , TANABE, Masashige , OHNOGI, Hiromu , SAGAWA, Hiroaki , KATO, Ikunoshin
摘要: The present invention relates to a therapeutic agent or prophylactic agent for a disease accompanying an abnormality in an amount of insulin or insulin response, an insulin-mimetic action agent, a food, beverage, or feed, an agent for enhancement of glucose uptake into a cell, and an agent for inducing differentiation into an adipocyte, each comprising as an effective ingredient at least one compound selected from the group consisting of specified compounds having an insulin-mimetic action, derivatives thereof, and pharmacologically acceptable salts thereof.
摘要翻译: 本发明涉及一种伴随胰岛素或胰岛素反应量异常的疾病的治疗剂或预防剂,胰岛素模拟作用剂,食物,饮料或饲料,用于增强葡萄糖摄入 细胞和用于诱导分化成脂肪细胞的药剂,其各自包含至少一种选自具有胰岛素模拟作用的特定化合物,其衍生物和其药理学上可接受的盐中的至少一种化合物作为有效成分。
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公开(公告)号:EP1623704A1
公开(公告)日:2006-02-08
申请号:EP04730641.0
申请日:2004-04-30
申请人: TAKARA BIO INC.
发明人: ENOKI, Tatsuji , KOBAYASHI, Eiji , OGAWA, Kinuko , KUDO, Yoko , TANABE, Masashige , SUGIYAMA, Katsumi , OHNOGI, Hiromu , SAGAWA, Hiroaki , KATO, Ikunoshin
IPC分类号: A61K31/121 , A61K31/222 , A61K31/343 , A61K31/341 , A61K31/365 , A61K31/351 , A61K31/381 , A61K31/366 , A61K31/352 , A61P43/00 , A61P3/10 , A61P3/08 , A61P5/50 , C07D493/08 , C07D309/12 , C07D311/32 , C07C49/84 , C07C49/835 , C07C69/18 , C07C69/157 , C07C69/16
CPC分类号: A61K31/365 , A23K20/111 , A23K20/116 , A23L33/10 , A61K31/121 , A61K31/222 , A61K31/341 , A61K31/343 , A61K31/351 , A61K31/352 , A61K31/366 , A61K31/381 , C07C49/84 , C07C69/017 , C07D307/46
摘要: A therapeutic agent and prophylactic agent for a disease accompanying an abnormality in an amount of insulin or insulin response, an agent for an insulin-mimetic action, a food, beverage and feed, an agent for enhancing glucose uptake into a cell, and an agent for inducing differentiation into an adipocyte, characterized in that each comprises as an effective ingredient at least one compound selected from the group consisting of a chalcone compound, an acetophenone compound, a coumarin compound, a phthalide compound, derivatives thereof, and pharmacologically acceptable salts thereof.
摘要翻译: 用于伴随胰岛素或胰岛素反应量异常的疾病的治疗剂和预防剂,胰岛素模拟作用剂,食品,饮料和饲料,用于增强葡萄糖摄入细胞的药剂和药剂 用于诱导分化为脂肪细胞的方法,其特征在于,每种包含至少一种选自查耳酮化合物,苯乙酮化合物,香豆素化合物,苯酞化合物,其衍生物和其药理学上可接受的盐中的至少一种化合物作为有效成分 。
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公开(公告)号:EP1510575A1
公开(公告)日:2005-03-02
申请号:EP03730703.0
申请日:2003-05-30
申请人: TAKARA BIO INC.
发明人: KOBAYASHI, Eiji , ENOKI, Tatsuji , TANABE, Masashige , UEDA, Yu , OKUDA, Shinji , SAGAWA, Hiroaki , KATO, Ikunoshin
CPC分类号: C12Q1/6883 , C12Q1/6827 , C12Q2600/156 , C12Q2531/119 , C12Q2525/121 , C12Q2521/319
摘要: Accurate and highly reproducible means of detecting a base substitution mutation (for example, SNP), an insertion mutation or a deletion mutation with the use of a nucleic acid sample in a trace amount and a method of typing gene polymorphisms using the means.
摘要翻译: 使用微量的核酸样品检测碱基取代突变(例如SNP),插入突变或缺失突变的精确和高度可重复的方法以及使用该方法分型基因多态性的方法。
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