-
公开(公告)号：EP0613946B1
公开(公告)日：2003-05-28
申请号：EP94102093.5
申请日：1994-02-11
IPC分类号： C12N15/12 , C07K14/475 , A61K38/18 , A61P7/00
-
2.发明公开hst-2 Mutein, DNA coding for the same and preparation thereof 失效HST-2 Mutein，dafürkodierende DNS和Herstellung。
公开(公告)号：EP0613946A2
公开(公告)日：1994-09-07
申请号：EP94102093.5
申请日：1994-02-11
摘要： The present invention provides a polypeptide represented by the following amino acid sequence:
wherein n is 0 or 1 and X represents
or a fragment thereof (n = 0: SEQ ID NO:1, n = 1: SEQ ID NO:2), a recombinant DNA coding for the polypeptide, a vector containing the recombinant DNA, the preparation of a transformant carrying the vector, and the production of the polypeptide with the transformant.
The invention also provides a method for promoting an increase in the number of platelets in a mammal using a polypeptide having a heparin binding secretory transforming factor 2 (hst-2) activity.
The polypeptide can be produced in large amounts, and has hst-2 activity, so that it can be advantageously used as drugs for promoting treatment of burns, wounds, etc., and therapeutic agents for gastrointestinal ulcers. Further, it can be utilized as platelet-increasing agents.摘要翻译： 本发明提供由以下氨基酸序列表示的多肽：其中n为0或1，X代表其片段（n = 0：SEQ ID NO：1，n = 1：SEQ ID NO： NO：2），编码该多肽的重组DNA，含有该重组DNA的载体，携带该载体的转化体的制备以及与转化体的多肽的产生。 本发明还提供使用具有肝素结合分泌转化因子2（hst-2）活性的多肽促进哺乳动物血小板数量增加的方法。 该多肽可以大量生产，并具有hst-2活性，因此可有利地用作促进烧伤，伤口等治疗药物和胃肠溃疡治疗剂的药物。 此外，它可以用作血小板递增剂。
-
公开(公告)号：EP0613946A3
公开(公告)日：1995-11-02
申请号：EP94102093.5
申请日：1994-02-11
摘要： The present invention provides a polypeptide represented by the following amino acid sequence:
wherein n is 0 or 1 and X represents
or a fragment thereof (n = 0: SEQ ID NO:1, n = 1: SEQ ID NO:2), a recombinant DNA coding for the polypeptide, a vector containing the recombinant DNA, the preparation of a transformant carrying the vector, and the production of the polypeptide with the transformant. The invention also provides a method for promoting an increase in the number of platelets in a mammal using a polypeptide having a heparin binding secretory transforming factor 2 (hst-2) activity. The polypeptide can be produced in large amounts, and has hst-2 activity, so that it can be advantageously used as drugs for promoting treatment of burns, wounds, etc., and therapeutic agents for gastrointestinal ulcers. Further, it can be utilized as platelet-increasing agents.-
4.发明公开Production of human nerve growth factor proteins 失效Menschliche Nervenwachstumsfaktoren und deren Produktion。
公开(公告)号：EP0414151A1
公开(公告)日：1991-02-27
申请号：EP90115815.4
申请日：1990-08-17
发明人： Kakinuma, Atsushi , Nakahama, Kazuo , Yoshimura, Koji , Sasada, Reiko , Kaisho, Yoshihiko , Iwane, Makoto
CPC分类号： C07K14/48
摘要： Disclosed are (1) an active human NGF protein having 6 cysteine residues in its molecule, in which the first cysteine residue from the N-terminus is bound to the fourth cysteine residue, the second cysteine residue to the fifth cysteine residue, and the third cysteine residue to the sixth cysteine residue to form disulfide bonds, (2) an animal cell which is transformed with a vector containing a gene coding for a human NGF protein and is cloned, (3) the animal cell described in (2), in which a host is a Chinese hamster CHO cell, and (4) a method for producing a human NGF protein which comprises cultivating the animal cell described in (2) or (3) in a medium. The human NGF proteins having specific disulfide bonds can be advantageously used as reagents or therapeutic drugs, and the cloned animal cells can commercially produce the high pure human NGF proteins in large amounts.
摘要翻译： 公开了（1）在其分子中具有6个半胱氨酸残基的活性人NGF蛋白，其中来自N末端的第一半胱氨酸残基与第四半胱氨酸残基，第二半胱氨酸残基连接至第五半胱氨酸残基，第三半胱氨酸残基与第五半胱氨酸残基相连， 半胱氨酸残基至第六半胱氨酸残基以形成二硫键，（2）用含有编码人NGF蛋白的基因的载体转化的动物细胞，并克隆，（3）（2）中描述的动物细胞， 宿主是中国仓鼠CHO细胞，以及（4）生产人NGF蛋白的方法，其包括在培养基中培养（2）或（3）中描述的动物细胞。 具有特异性二硫键的人NGF蛋白质可以有利地用作试剂或治疗药物，并且克隆的动物细胞可以大量商业生产高纯度人NGF蛋白质。
-
公开(公告)号：EP0414151B1
公开(公告)日：1995-03-15
申请号：EP90115815.4
申请日：1990-08-17
发明人： Kakinuma, Atsushi , Nakahama, Kazuo , Yoshimura, Koji , Sasada, Reiko , Kaisho, Yoshihiko , Iwane, Makoto
CPC分类号： C07K14/48
-
公开(公告)号：EP0317209B1
公开(公告)日：1993-02-10
申请号：EP88310665.0
申请日：1988-11-11
CPC分类号： C12N9/2462 , C07K14/485 , C07K2319/00 , C07K2319/02 , C12N15/81 , C12R1/865 , Y10S435/942
-
公开(公告)号：EP0317209A3
公开(公告)日：1989-10-18
申请号：EP88310665.0
申请日：1988-11-11
CPC分类号： C12N9/2462 , C07K14/485 , C07K2319/00 , C07K2319/02 , C12N15/81 , C12R1/865 , Y10S435/942
摘要： Disclosed are respiratory-deficient yeast except Saccharomyces cerevisiae AH22R⁻ , said respiratory-deficient yeast being transformed with a DNA containing a gene encoding a protein foreign to yeast and a method for preparing a protein foreign to yeast described above, which comprises culturing the yeast, and producing and accumulating the protein in a culture. The respiratory-deficient yeast of the present invention can produce a higher amount of protein than its parent strain.
-
公开(公告)号：EP0317209A2
公开(公告)日：1989-05-24
申请号：EP88310665.0
申请日：1988-11-11
CPC分类号： C12N9/2462 , C07K14/485 , C07K2319/00 , C07K2319/02 , C12N15/81 , C12R1/865 , Y10S435/942
摘要： Disclosed are respiratory-deficient yeast except Saccharomyces cerevisiae AH22R⁻ , said respiratory-deficient yeast being transformed with a DNA containing a gene encoding a protein foreign to yeast and a method for preparing a protein foreign to yeast described above, which comprises culturing the yeast, and producing and accumulating the protein in a culture. The respiratory-deficient yeast of the present invention can produce a higher amount of protein than its parent strain.
摘要翻译： 公开了除了酿酒酵母AH22R之外的呼吸缺陷酵母，所述呼吸缺陷酵母用含有编码酵母菌外源蛋白质的基因的DNA转化，以及制备上述酵母菌外源蛋白质的方法，包括培养 酵母，并在培养物中产生和积累蛋白质。 本发明的呼吸缺陷型酵母可产生比其亲本菌株更高量的蛋白质。
-
公开(公告)号：EP0386752B1
公开(公告)日：1994-09-14
申请号：EP90104419.8
申请日：1990-03-08
CPC分类号： C07K16/22 , A61K38/00 , C07K14/475 , C07K14/48
-
公开(公告)号：EP0386752A1
公开(公告)日：1990-09-12
申请号：EP90104419.8
申请日：1990-03-08
CPC分类号： C07K16/22 , A61K38/00 , C07K14/475 , C07K14/48
摘要： Disclosed are (1) a polypeptide (I) including the following amino acid sequence (II) in a molecule thereof:
TyrAlaGluHisLysSerHisArgGlyGluTyrSerValCys
AspSerGluSerLeuTrpValThrAspLysSerSerAlaIle
AspIleArgGlyHisGlnValThrValLeuGlyGluIleLys
ThrGlyAsnSerProValLysGlnTyrPheTyrGluThrArg
CysLysGluAlaArgProValLysAsnGlyCysArgGlyIle
AspAspLysHisTrpAsnSerGlnCysLysThrSerGlnThr
TyrValArgAlaLeuThrSerGluAsnAsnLysLeuValGly
TrpArgTrpIleArgIleAspThrSerCysValCysAlaLeu
SerArgLysIleGlyArg (II)
(2) a DNA sequence coding for the polypeptide described in (1), (3) a vector including the DNA described in (2), (4) a transformant transformed by the vector described in (3), and (5) a process for producing the polypeptide (I) which comprises cultivating the transformant described in (4) in a culture medium to produce and accumulate the polypeptide described in (1) in a culture. The polypeptide is useful as a reagent for studies relating to the differentiation, growth and survival of animal cells, and may also be useful as a drug.摘要翻译： 本发明公开了（1）的多肽（I），包括在其分子中的下列氨基酸序列（II）：TyrAlaGluHisLysSerHisArgGlyGluTyrSerValCys AspSerGluSerLeuTrpValThrAspLysSerSerAlaIle AspIleArgGlyHisGlnValThrValLeuGlyGluIleLys ThrGlyAsnSerProValLysGlnTyrPheTyrGluThrArg CysLysGluAlaArgProValLysAsnGlyCysArgGlyIle AspAspLysHisTrpAsnSerGlnCysLysThrSerGlnThr TyrValArgAlaLeuThrSerGluAsnAsnLysLeuValGly TrpArgTrpIleArgIleAspThrSerCysValCysAlaLeu SerArgLysIleGlyArg（II）（2）的DNA序列编码中所述的多肽（1 ），（3）包含（2），（4）中描述的DNA的载体，由（3）中描述的载体转化的转化体，和（5）生产多肽（I）的方法，其包括培养所述转化体 在（4）中，在培养基中产生并积累（1）中描述的多肽。 该多肽可用作关于动物细胞的分化，生长和存活的研究的试剂，并且也可用作药物。
-
-
-
-
-
-
-
-
-
搜索结果
15 条记录 (耗时 0.017 秒)
