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1.发明公开BENZYLISOQUINOLINE ALKALOID (BIA) PRECURSOR PRODUCING MICROBES, AND METHODS OF MAKING AND USING THE SAME 审中-公开生产微生物的苯甲酰喹啉生物碱（BIA）前体，以及制备和使用该生物微生物的方法
公开(公告)号：EP3292203A1
公开(公告)日：2018-03-14
申请号：EP16790024.0
申请日：2016-05-04
CPC分类号： C12N15/52 , C07D217/18 , C07D217/22 , C12N15/79 , C12N15/81 , C12P13/22 , C12P17/12 , C12Y114/16002 , C12Y401/01028
摘要： Methods and engineered yeast cells for generating a benzylisoquinoline alkaloid product are provided herein. A method comprises providing engineered yeast cells and a feedstock to a reactor. In the reactor, the engineered yeast cells are subjected to fermentation by incubating the engineered yeast cells for a time period to produce a solution comprising the BIA product and cellular material. The solution comprises not more than one class of molecule selected from the group of protoberberine, morphinan, isopavine, aporphine, and benzylisoquinoline. Additionally, at least one separation unit is used to separate the BIA product from the cellular material to provide the product stream comprising the BIA product.
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公开(公告)号：EP4253525A3
公开(公告)日：2023-10-18
申请号：EP23167022.5
申请日：2016-05-09
摘要： A method of epimerizing an (S) -1-benzylisoquinoline alkaloid to an (R) -1-benzylisoquinoline alkaloid is provided. The method comprises contacting the (S) -1-benzylisoquinoline alkaloid with at least one enzyme. Contacting the (S) -1-benzylisoquinoline alkaloid with the at least one enzyme converts the (S) -1-benzylisoquinoline alkaloid to an (R) -1-benzylisoquinoline alkaloid.
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公开(公告)号：EP4253525A2
公开(公告)日：2023-10-04
申请号：EP23167022.5
申请日：2016-05-09
摘要： A method of epimerizing an (S) -1-benzylisoquinoline alkaloid to an (R) -1-benzylisoquinoline alkaloid is provided. The method comprises contacting the (S) -1-benzylisoquinoline alkaloid with at least one enzyme. Contacting the (S) -1-benzylisoquinoline alkaloid with the at least one enzyme converts the (S) -1-benzylisoquinoline alkaloid to an (R) -1-benzylisoquinoline alkaloid.
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4.发明公开
公开(公告)号：EP3865580A1
公开(公告)日：2021-08-18
申请号：EP20212237.0
申请日：2014-03-14
摘要： Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
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5.发明公开
公开(公告)号：EP3553177A1
公开(公告)日：2019-10-16
申请号：EP19156536.5
申请日：2014-03-14
摘要： Aspects of the invention include host cells that are engineered to produce benzylisoquinoline alkaloids (BIAs). The host cells include heterologous coding sequences for a variety of enzymes involved in synthetic pathways from starting compounds to BIAs of the host cell. Also provided are methods of producing the BIAs of interest by culturing the host cells under culture conditions that promote expression of enzymes encoded by the heterologous coding sequences of the host cells. Aspects of the invention further include compositions, e.g., host cells, starting compounds and kits, etc., that find use in methods of the invention.
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