公开(公告)号：EP3189136A1

公开(公告)日：2017-07-12

申请号：EP15838658.1

申请日：2015-09-08

申请人： The Research Foundation for The State University of New York

发明人： WIMMER, Eckard ,  MUELLER, Steffen ,  FUTCHER, Bruce ,  SHEN, Sam ,  STAUFT, Charles ,  WARD, Charles

IPC分类号： C12N7/00 ,  C12N7/01 ,  A61K39/12 ,  A01N65/00

CPC分类号： C12N7/00 ,  A61K39/12 ,  A61K2039/5254 ,  C12N2760/12234 ,  C12N2760/12262 ,  C12N2760/20062 ,  C12N2770/24121 ,  C12N2770/24122 ,  C12N2770/24134 ,  C12N2770/24162 ,  C12N2800/22 ,  Y02A50/386

摘要： This invention provides an attenuated virus comprising a modified viral genome engineered to containing multiple nucleotide substitutions that reduce the codon pair bias of a virus protein encoding sequence relative to a first host while the codon pair bias relative to a second host is not substantially reduced. In another embodiment, the invention provides an attenuated virus comprising modified viral genome engineered to containing multiple nucleotide substitutions that reduce the codon pair bias of a virus protein-encoding sequence relative to a first host and a second host. The attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the attenuated virus.

摘要翻译： 本发明提供了包含经修饰的病毒基因组的减毒病毒，所述病毒基因组经工程改造而含有多个核苷酸置换，所述多个核苷酸置换降低病毒蛋白质编码序列相对于第一个宿主的密码子对偏倚，而相对于第二个宿主的密码子对偏倚实质上没有降低。 在另一个实施方案中，本发明提供了包含经修饰的病毒基因组的减毒病毒，所述病毒基因组经改造以含有多个核苷酸取代，所述多个核苷酸取代相对于第一宿主和第二宿主降低编码病毒蛋白的序列的密码子对偏倚。 减毒病毒可用于疫苗组合物中以在受试者中诱导保护性免疫应答。 本发明还提供了合成减毒病毒的方法。 此外，本发明进一步提供了用于预防对象患病毒相关疾病的方法，包括给予对象预防有效剂量的包含减毒病毒的疫苗组合物。

公开(公告)号：EP4368202A3

公开(公告)日：2024-08-21

申请号：EP23219605.5

申请日：2008-03-31

申请人： The Research Foundation for The State University of New York

发明人： WIMMER, Eckard ,  SKIENA, Steve ,  MUELLER, Steffen ,  FUTCHER, Bruce ,  PAPAMICHAIL, Dimitris ,  COLEMAN, John Robert ,  CELLO, Jeronimo

IPC分类号： A61K39/13 ,  A61K39/12

CPC分类号： A61K2039/525420130101 ,  C12N2720/1236120130101 ,  C12N2740/1506120130101 ,  C12N2760/1606120130101 ,  C12N2770/2006120130101 ,  C12N2770/2416120130101 ,  C12N2770/3206120130101 ,  C12N2770/3266120130101 ,  C12N2770/3276120130101 ,  A61K39/12 ,  C12N7/00 ,  A61P31/12 ,  A61P31/14 ,  A61P31/16 ,  A61P31/18 ,  A61P31/20 ,  A61P31/22 ,  A61P37/04 ,  Y02A50/30

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

公开(公告)号：EP4368202A2

公开(公告)日：2024-05-15

申请号：EP23219605.5

申请日：2008-03-31

申请人： The Research Foundation for The State University of New York

发明人： WIMMER, Eckard ,  SKIENA, Steve ,  MUELLER, Steffen ,  FUTCHER, Bruce ,  PAPAMICHAIL, Dimitris ,  COLEMAN, John Robert ,  CELLO, Jeronimo

IPC分类号： A61K39/12

CPC分类号： A61K2039/525420130101 ,  C12N2720/1236120130101 ,  C12N2740/1506120130101 ,  C12N2760/1606120130101 ,  C12N2770/2006120130101 ,  C12N2770/2416120130101 ,  C12N2770/3206120130101 ,  C12N2770/3266120130101 ,  C12N2770/3276120130101 ,  A61K39/12 ,  C12N7/00 ,  A61P31/12 ,  A61P31/14 ,  A61P31/16 ,  A61P31/18 ,  A61P31/20 ,  A61P31/22 ,  A61P37/04 ,  Y02A50/30

摘要： This invention provides an attenuated virus which comprises a modified viral genome containing nucleotide substitutions engineered in multiple locations in the genome, wherein the substitutions introduce synonymous deoptimized codons into the genome. The instant attenuated virus may be used in a vaccine composition for inducing a protective immune response in a subject. The invention also provides a method of synthesizing the instant attenuated virus. Further, this invention further provides a method for preventing a subject from becoming afflicted with a virus-associated disease comprising administering to the subject a prophylactically effective dose of a vaccine composition comprising the instant attenuated virus.

公开(公告)号：EP3370766A1

公开(公告)日：2018-09-12

申请号：EP16863171.1

申请日：2016-11-07

申请人： The Research Foundation for The State University of New York

发明人： FUTCHER, Bruce ,  GARDIN, Justin ,  SKIENA, Steve ,  YUROVSKY, Alisa ,  WIMMER, Eckard ,  MUELLER, Steffen

IPC分类号： A61K39/12 ,  C12N7/04 ,  A61P31/12

CPC分类号： C12N7/00

摘要： This invention provides a modified protein encoding sequence containing nucleotide substitutions at multiple locations in the protein encoding sequence, wherein the substitutions introduce rare hexamers. These hexamers may be Frame Dependent, or depleted in only the reading frame, or Frame Independent, or depleted in all three frames. Modified protein encoding sequences of the present invention may include modified viruses useful for vaccines.

公开(公告)号：EP2969005A1

公开(公告)日：2016-01-20

申请号：EP14763039.6

申请日：2014-03-15

申请人： The Research Foundation for The State University of New York

发明人： MUELLER, Steffen ,  WIMMER, Eckard ,  FUTCHER, Bruce ,  SKIENA, Steven ,  YANG, Chen

IPC分类号： A61P31/16 ,  C12N7/04

CPC分类号： C12N7/00 ,  A61K39/145 ,  C12N2760/16034 ,  C12N2760/16061 ,  C12N2760/16134 ,  C12N2760/16162

摘要： This invention provides highly attenuated influenza viruses and vaccines. The attenuated viruses and vaccines proliferate well and have high safety factors. The attenuated viruses providing protective immunity from challenge by virus of the same subtype, as well as cross protection against heterologous viruses.