公开(公告)号：EP4435109A1

公开(公告)日：2024-09-25

申请号：EP22895653.8

申请日：2022-11-16

申请人： Tokyo University of Pharmacy and Life Science ,  National University Corporation Tottori University

发明人： TOMIZUKA, Kazuma ,  UNO, Narumi ,  KAZUKI, Yasuhiro

IPC分类号： C12N15/85 ,  A01K67/027 ,  C12N5/10 ,  C12P21/00

CPC分类号： A01K67/027 ,  C12P21/00 ,  C12N15/85 ,  C12N5/10

摘要： An object of the present invention is to provide a promoter activation sequence that allows for stable and efficient expression of a DNA of interest in a mammalian cell. The present invention provides a promoter activation sequence comprising a nucleotide sequence represented by SEQ ID NO: 1, or a nucleotide sequence of 850 or more in a nucleotide sequence having 85% or higher sequence identity thereto; an expression vector comprising the promoter activation sequence, a promoter, and a DNA of interest; and a mammalian cell or a mammal comprising the expression vector.

公开(公告)号：EP3766980A1

公开(公告)日：2021-01-20

申请号：EP19767483.1

申请日：2019-03-15

申请人： National University Corporation Tottori University ,  Trans Chromosomics, Inc.

发明人： KAZUKI, Yasuhiro ,  OSHIMURA, Mitsuo ,  ABE, Satoshi

IPC分类号： C12N15/85 ,  A01K67/027 ,  C12N5/10 ,  C12N15/12 ,  C12N15/13 ,  C12N15/16

摘要： This application provides: a novel mouse artificial chromosome vector that is stable in rodent cells, tissues, or individuals, specifically a mouse artificial chromosome vector derived from a mouse chromosome selected from the group consisting of mouse chromosome 10 and mouse chromosome 16; a cell or a non-human animal comprising the vector; and use of the vector for production of proteins and human antibodies.

公开(公告)号：EP2218786B1

公开(公告)日：2013-09-18

申请号：EP08849922.3

申请日：2008-10-14

申请人： National University Corporation Tottori University ,  Chromocenter Inc.

发明人： OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  MATSUOKA, Takashi ,  TOMIZUKA, Kazuma ,  OSHIMA, Takeshi

IPC分类号： C12N15/85 ,  A01K67/027 ,  C12N5/10 ,  C12N9/02 ,  C12Q1/02 ,  G01N33/15 ,  G01N33/50

CPC分类号： C12N15/8509 ,  A01K67/0278 ,  A01K2217/00 ,  A01K2217/07 ,  A01K2227/105 ,  C12N9/0071 ,  C12N9/0077 ,  C12N2800/208 ,  G01N33/5088

公开(公告)号：EP3533867A1

公开(公告)日：2019-09-04

申请号：EP17864055.3

申请日：2017-10-31

申请人： National University Corporation Tottori University ,  Trans Chromosomics, Inc.

发明人： KAZUKI, Yasuhiro ,  ABE, Satoshi ,  OSHIMURA, Mitsuo

IPC分类号： C12N15/00 ,  A01K67/027 ,  C12N15/09 ,  C12P21/08

摘要： This application provides: a non-human animal that comprises a mouse artificial chromosome comprising a human antibody heavy chain gene or gene locus, a human antibody light chain κ gene or gene locus, and/or a human antibody light chain λ gene or gene locus, and in which endogenous antibody genes or gene loci corresponding to at least 2 human antibody genes or gene loci have been knocked out, wherein the animal can be stably retained through generations and can produce human antibodies; a method for producing the non-human animal; and a method for producing human antibodies using the non-human animal.

公开(公告)号：EP4215545A1

公开(公告)日：2023-07-26

申请号：EP21869483.4

申请日：2021-09-16

申请人： National University Corporation Tottori University

发明人： TAKAHASHI, Yoshimasa ,  ONODERA, Taishi ,  ADACHI, Yu ,  OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  SATOFUKA, Hiroyuki ,  HASHIGUCHI, Takao ,  MAENAKA, Katsumi

IPC分类号： C07K16/10 ,  A61K39/395 ,  A61P31/14 ,  C07K16/46 ,  C12N15/13 ,  G01N33/53 ,  G01N33/569

摘要： Provided is a human antibody against a spike protein of coronavirus, in particular, against a protein in an extracellular region or a receptor-binding domain.

公开(公告)号：EP2522725A1

公开(公告)日：2012-11-14

申请号：EP11731868.3

申请日：2011-01-06

申请人： National University Corporation Tottori University ,  Chromocenter Inc.

发明人： OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  TAKIGUCHI, Masato ,  MATSUOKA, Takashi

IPC分类号： C12N15/00 ,  A01K67/027 ,  C12N15/09 ,  C12N15/873 ,  C12N5/0735 ,  C12N5/074

CPC分类号： A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/01 ,  C12N15/1086 ,  C12N15/85 ,  C12N15/8509 ,  C12N2800/208 ,  C12N2800/30

摘要： Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.

摘要翻译： 公开了一种小鼠人造染色体载体，其包含：衍生自小鼠染色体的天然着丝粒; 通过在接近着丝粒的小鼠染色体长臂位点缺失长臂远端区而形成的小鼠染色体衍生的长臂片段; 和端粒序列，其中载体稳定地保留在哺乳动物的细胞和/或组织中。 此外，所公开的是包含载体的细胞或非人动物，以及细胞或非人动物的使用。

公开(公告)号：EP2767160B1

公开(公告)日：2017-01-18

申请号：EP12840420.9

申请日：2012-10-12

申请人： Phoenixbio Co., Ltd. ,  National University Corporation Tottori University

发明人： OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  MUKAIDANI, Chise ,  SHIMADA, Takashi ,  KAKUNI, Masakazu ,  HAMAMURA, Satoko ,  KAMIMURA, Hidetaka ,  KAWAMURA, Akio ,  NAKADA, Naoyuki ,  OHBUCHI, Masato ,  KATO, Kota

IPC分类号： A01K67/027 ,  G01N33/15 ,  G01N33/50

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/0042 ,  G01N33/5067 ,  G01N33/5088

公开(公告)号：EP2522725B1

公开(公告)日：2016-10-05

申请号：EP11731868.3

申请日：2011-01-06

申请人： National University Corporation Tottori University ,  Chromocenter Inc.

发明人： OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  TAKIGUCHI, Masato ,  MATSUOKA, Takashi

IPC分类号： C12N15/85 ,  A01K67/027 ,  C12N15/10

CPC分类号： A01K67/0278 ,  A01K2207/15 ,  A01K2217/00 ,  A01K2217/052 ,  A01K2217/206 ,  A01K2227/105 ,  A01K2267/01 ,  C12N15/1086 ,  C12N15/85 ,  C12N15/8509 ,  C12N2800/208 ,  C12N2800/30

摘要： Disclosed is a mouse artificial chromosome vector, comprising: a natural centromere derived from a mouse chromosome; a mouse-chromosome-derived long-arm fragment formed by deleting a long-arm distal region at a mouse chromosome long-arm site proximal to the centromere; and a telomere sequence, wherein the vector is stably retained in a cell and/or tissue of a mammal. In addition, disclosed are cells or non-human animals comprising the vector, and use of the cells or non-human animals.

公开(公告)号：EP2767160A1

公开(公告)日：2014-08-20

申请号：EP12840420.9

申请日：2012-10-12

申请人： Phoenixbio Co., Ltd. ,  National University Corporation Tottori University

发明人： OSHIMURA, Mitsuo ,  KAZUKI, Yasuhiro ,  MUKAIDANI, Chise ,  SHIMADA, Takashi ,  KAKUNI, Masakazu ,  HAMAMURA, Satoko ,  KAMIMURA, Hidetaka ,  KAWAMURA, Akio ,  NAKADA, Naoyuki ,  OHBUCHI, Masato ,  KATO, Kota

IPC分类号： A01K67/027 ,  G01N33/15 ,  G01N33/50

CPC分类号： A01K67/0271 ,  A01K2207/12 ,  A01K2217/075 ,  A01K2227/105 ,  A01K2267/03 ,  C12N9/0042 ,  G01N33/5067 ,  G01N33/5088

摘要： A chimeric non-human animal having an in vivo human hepatocyte population, wherein the effects of non-human animal cells on drug metabolism are suppressed or deleted is provided. A method for producing a chimeric non-human animal that lacks a drug-metabolizing system or has a suppressed drug-metabolizing system and is provided with a drug-metabolizing system driven by human hepatocytes, is provided. The method comprises transplanting human hepatocytes into a non-human animal characterized by (i) being immunodeficient, (ii) having liver damage, and (iii) lacking the functions of an endogenous Cyp3a gene.

摘要翻译： 提供了具有体内人肝细胞群体的嵌合非人动物，其中非人动物细胞对药物代谢的作用被抑制或缺失。 提供了一种制备缺乏药物代谢系统或具有抑制药物代谢系统并具有由人肝细胞驱动的药物代谢系统的嵌合非人动物的方法。 该方法包括将人肝细胞移植到非人动物中，其特征在于：（i）免疫缺陷，（ii）具有肝损伤，和（iii）缺乏内源性Cyp3a基因的功能。

公开(公告)号：EP4242317A1

公开(公告)日：2023-09-13

申请号：EP21889268.5

申请日：2021-11-05

申请人： Trans Chromosomics, Inc. ,  National University Corporation Tottori University

发明人： OSHIMURA, Mitsuo ,  TABATA, Toshiaki ,  KAZUKI, Yasuhiro ,  UNO, Narumi

IPC分类号： C12N15/86 ,  C12N5/10

摘要： An object of the present invention is to provide a method for producing a reversibly immortalized cell which can allow a cell into which an immortalizing gene is introduced to proliferate over a long period without damaging the chromosome of the cell, and is capable of removing the immortalizing gene, and to provide a method for obtaining a large amount of a reversibly immortalized cell that can be cloned and has stable quality. The present invention provides a method for producing a reversibly immortalized cell, comprising the steps of introducing a chromosomally non-integrated RNA virus vector loaded with one or two or more immortalizing gene(s), selected from the group consisting of Bmi-1 gene, TERT gene, and SV40T gene, into a mammalian cell so that the immortalizing gene is expressed in the cell; and culturing the obtained cell for proliferation.