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    MICROFLUIDIC DEVICE FOR THE DETERMINATION OF CELLULAR MRNA
    3.
    发明公开
    MICROFLUIDIC DEVICE FOR THE DETERMINATION OF CELLULAR MRNA 审中-公开

    公开(公告)号：EP4184515A1

    公开(公告)日：2023-05-24

    申请号：EP22199379.3

    申请日：2018-08-13

    申请人： The Governing Council of the University of Toronto

    发明人： LABIB, Mahmoud ,  SARGENT, Edward ,  KELLEY, Shana

    IPC分类号： G16B25/00 ,  C12Q1/6841 ,  B01L3/00

    摘要： Methods and systems for mRNA analysis and quantification of mRNA expression in cells are provided. An example method includes introducing a first capture probe and a second capture probe into the cells, the first capture probe and the second capture probe each configured to be complementary to a respective section of target mRNA within the cells, wherein binding of the first and second capture probes to the respective sections of the target mRNA results in tagging of the cells and causes the first and second capture probes to form clusters with each other. The first capture probe and the second capture probe are each bound to magnetic nanoparticles (MNPs) that, when trapped within the tagged cells, cause the tagged cells to be susceptible to magnetic forces. The method and system further include introducing the cells into a device configured to magnetically capture tagged cells.