公开(公告)号：EP3527280A1

公开(公告)日：2019-08-21

申请号：EP19151505.5

申请日：2014-09-02

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： GUNDLACH, Jens H. ,  LASZLO, Andrew ,  DERRINGTON, Ian ,  MANDELL, Jeffrey G.

IPC分类号： B01D57/02 ,  B01D59/42 ,  C07H21/02 ,  C12P21/06

摘要： The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：EP3038738B1

公开(公告)日：2019-02-27

申请号：EP14841122.6

申请日：2014-09-02

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： GUNDLACH, Jens H. ,  LASZLO, Andrew ,  DERRINGTON, Ian ,  MANDELL, Jeffrey G.

IPC分类号： C12Q1/6869

公开(公告)号：EP3038738A1

公开(公告)日：2016-07-06

申请号：EP14841122.6

申请日：2014-09-02

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： GUNDLACH, Jens H. ,  LASZLO, Andrew ,  DERRINGTON, Ian ,  MANDELL, Jeffrey G.

IPC分类号： B01D57/02 ,  B01D59/42 ,  C07H21/02 ,  C12P21/06

CPC分类号： C12Q1/6869 ,  B01D57/02 ,  G01N33/48721 ,  C12Q2521/101 ,  C12Q2523/303 ,  C12Q2525/107 ,  C12Q2525/113 ,  C12Q2525/119 ,  C12Q2565/631 ,  C12Q2523/125

摘要： The present disclosure provides method and systems for improving nanopore-based analysis of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

摘要翻译： 本公开提供了用于改进基于纳米孔的聚合物分析的方法和系统。 本公开提供了用于选择性地修饰一种或多种单分子的方法，所述单体亚单位使得分析物聚合物与修饰的亚基形成聚合物分析物。 聚合物分析物在基于纳米孔的系统中产生可检测的信号。 可检测的信号和/或其与参考信号的偏差指示经修饰的亚基在聚合物分析物中的位置，因此允许在原始分析物内聚合物的该位置识别亚基。

公开(公告)号：EP3936222A1

公开(公告)日：2022-01-12

申请号：EP21179889.7

申请日：2014-09-02

申请人： University of Washington through its Center for Commercialization ,  Illumina, Inc.

发明人： GUNDLACH, Jens H. ,  LASZLO, Andrew ,  DERRINGTON, Ian ,  MANDELL, Jeffrey G.

IPC分类号： B01D57/02 ,  B01D59/42 ,  C07H21/02 ,  C12P21/06 ,  C12Q1/6869 ,  G01N33/487

摘要： The present disclosure provides method and systems for improving nanopore-based analyses of polymers. The disclosure provides methods for selectively modifying one or more monomeric subunit(s) of a kind a pre-analyte polymer that results polymer analyte with a modified subunit. The polymer analyte produces a detectable signal in a nanopore-based system. The detectable signal, and/or its deviation from a reference signal, indicates the location of the modified subunit in the polymer analyte and, thus, permits the identification of the subunit at that location in the original pre-analyte polymer.

公开(公告)号：EP4457368A2

公开(公告)日：2024-11-06

申请号：EP22917567.4

申请日：2022-12-29

申请人： Illumina, Inc.

发明人： MANZO, Andrea ,  KARUNAKARAN, Aathavan ,  FISHER, Jeffrey S. ,  MANDELL, Jeffrey G. ,  VERMAAS, Eric Hans ,  KAPER, Fiona ,  WHITE, Adam ,  PRICE, Andrew J. ,  OSTROW, Andrew Zachary ,  BRODIN, Jeffrey ,  SHULTZABERGER, Sarah Munchel

IPC分类号： C12Q1/6881 ,  C12Q1/6809 ,  C12N15/10 ,  C12N9/12 ,  C12Q1/686 ,  C12Q1/6841 ,  C12Q1/6874

公开(公告)号：EP3175237B1

公开(公告)日：2020-03-04

申请号：EP15748142.5

申请日：2015-07-29

申请人： Illumina, Inc.

发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

IPC分类号： G01N33/487

公开(公告)号：EP4379373A3

公开(公告)日：2024-07-10

申请号：EP24167857.2

申请日：2015-07-29

申请人： Illumina, Inc.

发明人： BOYANOV, Boyan ,  MANDELL, Jeffrey G. ,  GUNDERSON, Kevin L. ,  BAI, Jingwei ,  QIANG, Liangliang ,  BAAS, Bradley

IPC分类号： G01N33/487

CPC分类号： G01N33/48721

摘要： Provided is a system for detection of nucleic acid, the system comprising:
(i) a detection apparatus, wherein the detection apparatus comprises one or more protein nanopores and (ii) an amplifier configured to amplify electrical signals generated at the nanopore and/or a computer or data acquisition circuit coupled to the detection apparatus to evaluate signals detected from the protein nanopore. In one aspect, the detection apparatus comprises a solid support with an array of solid-state nanopores and a plurality of lipid nanodiscs with protein nanopores, wherein the lipid nanodiscs are disposed on the solid support such that they form seals at the solid-state nanopores. In another aspect, the detection apparatus comprises one or more nanopores that are surrounded by a membrane, wherein each nanopore is tethered to an electrode. Also provided are methods of sequencing nucleic acids using the systems.

公开(公告)号：EP3942067A2

公开(公告)日：2022-01-26

申请号：EP20754915.5

申请日：2020-01-24

申请人： Illumina, Inc.

发明人： FISHER, Jeffrey S. ,  MATHER, Brian D. ,  PUGLIESE, Kaitlin M. ,  MANDELL, Jeffrey G. ,  ROGERT BACIGALUPO, Maria Candelaria ,  BOYANOV, Boyan

IPC分类号： C12Q1/6825 ,  C12Q1/6834 ,  G01N27/30

公开(公告)号：EP3172321B1

公开(公告)日：2019-08-21

申请号：EP15753229.2

申请日：2015-07-20

申请人： Illumina, Inc.

发明人： CANN, Gordon ,  MANDELL, Jeffrey G. ,  ARAVANIS, Alex ,  NORBERG, Steven ,  POKHOLOK, Dimitry K. ,  STEEMERS, Frank. J. ,  ABSALAN, Farnaz ,  BAZARGAN, Leila

IPC分类号： C12N15/10 ,  C12Q1/68

公开(公告)号：EP3172321A1

公开(公告)日：2017-05-31

申请号：EP15753229.2

申请日：2015-07-20

申请人： Illumina, Inc.

发明人： CANN, Gordon ,  MANDELL, Jeffrey G. ,  ARAVANIS, Alex ,  NORBERG, Steven ,  POKHOLOK, Dimitry K. ,  STEEMERS, Frank. J. ,  ABSALAN, Farnaz ,  BAZARGAN, Leila

IPC分类号： C12N15/10 ,  C12Q1/68

CPC分类号： C12P19/34 ,  C12N15/102 ,  C12Q1/6816 ,  C12Q1/683 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2522/101 ,  C12Q2563/131

摘要： A method for enriching a target nucleic acid comprising providing an endonuclease system having a crRNA or a derivative thereof, and a Cas protein or a variant thereof. The crRNA or the derivative thereof contains a target-specific nucleotide region substantially complementary to a region of the target nucleic acid; contacting the target nucleic acid with the endonuclease system to form a complex; and separating the complex and thereby enriching for the target nucleic acid.

摘要翻译： 用于富集靶核酸的方法包括提供具有crRNA或其衍生物和Cas蛋白或其变体的核酸内切酶系统。 crRNA或其衍生物包含与靶核酸的区域基本上互补的靶特异性核苷酸区域; 使靶核酸与内切核酸酶系统接触以形成复合物; 并分离复合物并由此富集靶核酸。