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    BIOLUMINESCENT PLANTS AND METHODS OF MAKING SAME
    8.
    发明公开
    BIOLUMINESCENT PLANTS AND METHODS OF MAKING SAME 审中-公开
    维多利亚祖鲁尼亚

    公开(公告)号：EP1383372A4

    公开(公告)日：2004-06-30

    申请号：EP02763991

    申请日：2002-04-08

    申请人： SCRIPPS RESEARCH INST

    发明人： KAY STEVE A ,  KUHLMANN TINA ,  LERNER RICHARD A

    IPC分类号： A01H5/00 ,  C12N5/10 ,  C12N15/09 ,  C12N15/12 ,  C12N15/82 ,  A01H5/10 ,  C12N15/52

    CPC分类号： C12N15/8241

    摘要： A genetically modified plant cell containing a heterologous nucleotide sequence encoding a bioluminescent polypeptide, which is expressed in amount sufficient to produce at least about 750,000 photons of visible light/mm2/second, is provided, as is a visibly bioluminescent transgenic plant, which contains such a genetically modified plant cell. Also provided is a recombinant nucleic acid molecule, which contains a plant translational enhancer operatively linked to a nucleotide sequence encoding a bioluminescent polypeptide. In addition, methods of producing a genetically modified plant cell that is visibly bioluminescent introducing a transgene encoding a bioluminescent polypeptide into a plant cell, whereby the bioluminescent polypeptide is expressed at a level that produces at least about 750,000 photons of visible light/mm2/second are provided, as are kits containing such visibly bioluminescent compositions.

    摘要翻译： 提供了含有编码生物发光多肽的异源核苷酸序列的遗传修饰的植物细胞，其以足以产生至少约750000个可见光/ mm 2 /秒的光子的量表达，如可见生物发光转基因植物， 其含有这样的遗传修饰的植物细胞。 还提供了重组核酸分子，其包含与编码生物发光多肽的核苷酸序列可操作地连接的植物翻译增强子。 此外，生产显性生物发光的转基因植物细胞的方法将将编码生物发光多肽的转基因导入植物细胞，由此生物发光多肽以至少约75万光束的可见光/ mm 2表达 > /秒，以及含有这种可视生物发光组合物的试剂盒。