公开(公告)号：EP1164838A4

公开(公告)日：2002-06-19

申请号：EP00901885

申请日：2000-02-03

申请人： YEDA RES & DEV ,  PROCHON BIOTECH LTD

发明人： YAYON AVNER ,  REZNITSKY DALYA ,  BEN-LEVY RACHEL

IPC分类号： A61K49/00 ,  C07K14/71 ,  C12P21/04 ,  G01N33/50 ,  A01K63/00 ,  A61K48/00 ,  C12N1/20 ,  C12N5/00 ,  C12N5/02 ,  C12N15/00 ,  C12N15/09 ,  C12N15/63 ,  C12N15/70 ,  C12N15/74 ,  G01N33/53 ,  G01N33/567 ,  G01N33/574

CPC分类号： A61K49/0004 ,  C07K14/71 ,  G01N33/5011 ,  G01N2333/71

摘要： In vitro and in vivo screening assays for antagonists of fibroblast growth factor receptor (FGFR)-mediated malignant cell transformation are provided using stable cell lines genetically engineered to express a recombinant wild type or constitutively active mutant FGFR selected from FGFR1, FGFR2 and FGFR3, the malignant potential of said cell lines being modulated by said FGFR.

公开(公告)号：EP0836380A4

公开(公告)日：1999-03-31

申请号：EP96917641

申请日：1996-06-12

申请人： YEDA RES & DEV

发明人： YAYON AVNER

IPC分类号： C12N15/09 ,  A01K67/027 ,  A61K9/22 ,  A61K35/32 ,  A61K38/00 ,  A61K38/22 ,  A61K39/395 ,  A61K45/00 ,  A61L27/00 ,  A61P5/00 ,  A61P35/00 ,  A61P43/00 ,  C07K14/50 ,  C07K14/715 ,  C07K16/00 ,  C07K16/24 ,  C07K16/28 ,  C12N5/00 ,  C12N5/06 ,  C12P21/08 ,  C12Q1/02 ,  G01N33/53 ,  G01N33/558 ,  G01N33/566 ,  G01N33/569 ,  G01N33/68 ,  G01N33/74 ,  A01K67/00 ,  A61K39/00 ,  C12N15/00

CPC分类号： G01N33/566 ,  A61K38/00 ,  A61K2039/505 ,  C07K14/50 ,  C07K16/2863 ,  G01N33/53 ,  G01N33/56966 ,  G01N33/6887 ,  G01N33/74 ,  G01N2333/50 ,  G01N2333/9121

摘要： The present invention concerns fibroblast growth factor receptor 3 (FGFR3) as a novel marker for mesenchymal skeletal progenitor cells. By utilizing this novel marker it was possible both to identify and locate mesenchymal skeletal progenitor cells in a tissue, as well as to obtain a substantially pure culture of such cells. The pure culture of the mesenchymal skeletal progenitor cells may be used, optionally after various manipulations ex vivo, as an active ingredient in pharmaceutical compositions or implants for the purpose of bone and/or cartilage repair. FGFR3 may also be used as a marker for the identification and the localization of cartilage- and bone-derived tumors. Agents capable of binding to FGFR3 may also be used for targeting cytotoxic agents to cartillage- and bone-derived tumors.The present invention concerns fibroblast growth factor receptor 3 (FGFR3) as a novel marker for mesenchymal skeletal progenitor cells. By utilizing this novel marker it was possible both to identify and locate mesenchymal skeletal progenitor cells in a tissue, as well as to obtain a substantially pure culture of such cells. The pure culture of the mesenchymal skeletal progenitor cells may be used, optionally after various manipulations ex vivo, as an active ingredient in pharmaceutical compositions or implants for the purpose of bone and/or cartilage repair. FGFR3 may also be used as a marker for the identification and the localization of cartilage- and bone-derived tumors. Agents capable of binding to FGFR3 may also be used for targeting cytotoxic agents to cartillage- and bone-derived tumors.

公开(公告)号：EP0730651A4

公开(公告)日：1997-12-03

申请号：EP94929876

申请日：1994-09-23

申请人： AMERICAN CYANAMID CO ,  YEDA RES & DEV

发明人： SEDDON ANDREW PETER ,  LI LU-YUAN ,  BOEHLEN PETER ,  EISINGER MAGDALENA ,  YAYON AVNER

IPC分类号： C12N15/09 ,  A61K38/00 ,  A61K38/22 ,  A61P5/00 ,  A61P9/00 ,  A61P43/00 ,  C07K14/50 ,  C07K14/545 ,  C12N5/10 ,  C12N15/18 ,  C12P21/02 ,  C12R1/91 ,  A61K38/18 ,  A61K38/19 ,  A61K38/20 ,  C12N15/19 ,  C12N15/24 ,  C12N15/25

CPC分类号： C07K14/50 ,  A61K38/00 ,  C07K14/501 ,  C07K14/503 ,  C07K14/545

摘要： Structural analogues of fibroblast growth factors have an amino acid sequence replacement in the ninth or tenth beta strand of the factor, or the sequence that corresponds to the surface loop that connects the ninth and tenth beta strands, such that the folding of the molecule is not significantly perturbed. Preferred analogues have the overall secondary and tertiary structure of the original factor, and bind to heparin and a member or members of the fibroblast growth factor receptor family with high affinity. In some embodiments the replacement is with another amino acid sequence such as a loop sequence from another structurally related fibroblast growth factor or an interleukin. The figure discloses amino acid sequences from FGF-1, FGF-2, and IL-1 beta.