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公开(公告)号:EP1216100A1
公开(公告)日:2002-06-26
申请号:EP00964445.1
申请日:2000-09-29
发明人: LEE, Martin, Alan , BIRD, Hilary , LESLIE, Dario, LyallCBD Porton Down , SQUIRRELL, David, James , SHAW, JohnCentral Research Laboratories Limited , WENN, DavidCentral Research Laboratories Limited , DEACON,JulieCentral Research Laboratories Limited
摘要: A method of carrying out an amplification reaction, said method comprising supplying to a well in a disposable unit (a) a sample which contains or is suspected of containing a target nucleic acid sequence (b) primers, nucleotides and enzymes required to effect said amplification reaction and (c) a buffer system, and subjecting the unit to thermal cycling conditions such that any target nucleic acid present within the sample is amplified; wherein the disposable unit comprises a thermally conducting layer and a facing layer having one or more reagent wells of up to 1000 microns in depth defined therebetween; and the reaction mixture comprises at least one of the following: A) a buffer system wherein the pH is above 8.3; B) a detergent; and/or C) a blocking agent. Apparatus for effecting the method as well as disposable units for use in the method are described. The method is particularly suitable for rapid PCR reactions.
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公开(公告)号:EP0894285B1
公开(公告)日:2001-06-13
申请号:EP97916555.2
申请日:1997-04-14
IPC分类号: G02F1/139 , G02F1/1337
CPC分类号: G02F1/1396 , G02F1/133753 , G02F1/13725 , G02F2001/133742
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公开(公告)号:EP0891388A1
公开(公告)日:1999-01-20
申请号:EP97907224.0
申请日:1997-03-19
发明人: MEHL, Georg, Hans, Rudolf , GOODBY, John, William , TUFFIN, Rachel, Patricia , TOYNE, Kenneth, Johnson
CPC分类号: C09K19/406 , C08G77/38 , C09K19/408
摘要: Materials of formula (I) are provided wherein E=0 indicates a non-cyclic system, E=1 indicates a cyclic system; when E=0 then G is R3SiR1R2(O)k; R1, R2, R3 are independently selected from C1-16 alkyl which may be partly or fully halogenated, H and formula (IA) wherein Y is a propyl group which may be partially halogenated with fluorine or chlorine and Y1 is independently selected from COO, OCO, O, S, CHOH, CHF, CO, CH2; Q and Q1 are independently selected from (CH2)n wherein one or more non-adjacent methylenes may be replaced by O and N=0-20; Z and Z1 are independently selected from O, S, single covalent bond, COO, OCO; when Y and/or Y1 is CH2 then n may also be 0; Figure (2) represents any mesogenic group; and MACROCYCLE represents any macrocycle; provided that at least one of R1, R2, R3 is selected from formula (IA); k=0 or 1; g=4 or 6 or 8 or 10 or 12; l=3 or 8 provided that: if k=l, l=3 and x is selected from 6, 8, 10, 12 then g=x; if k=0, l=8 and g=4 then x=1; and further provided that G may have up to g different structures for any single compound.
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公开(公告)号:EP0573424B1
公开(公告)日:1996-02-07
申请号:EP91913549.1
申请日:1991-07-18
CPC分类号: C04B35/62227 , C23C16/46 , C23C16/545 , C30B11/12 , C30B25/005
摘要: A coreless refractory fibre is made introducing a filament (16) of a former material into a chemical vapour deposition (CVD) enclosure (10) and then heating the end (21) of the filament by means of a contactless heating source. The source comprises a conducting coil connected to an AC source in the frequency range HF to UHF and with a linear or strip conducting element (110) connected to it such that the end of the element is adjacent to the end (21) of the filament. The filament (16) is then withdrawn such that refractory material is continuously built up on its end from the chemical vapours in the enclosure to form a coreless refractory fibre. Whiskers of a coreless refractory material may be made by introducing fine particles of a catalyst material on to a platform (22) inside a CVD enclosure (25), melting the catalyst material to form droplets and then feeding CVD gases to the droplets (30) to thereby promote crystal growth by precipitation from the supersaturated liquid. The end of the precipitated material within the enclosure is heated by means of a linear conducting element vertically mounted and provided at its free end with a perpendicular conducting disc (111) adjacent and parallel to the platform (22). The platform is then moved vertically from the heat source such that the separation of the droplets (30) from the heating source (111) is maintained constant as chemical vapour is continuously deposited under the droplets (30) from the chemical vapours in the enclosure to form whiskers.
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公开(公告)号:EP0606320B1
公开(公告)日:1996-01-31
申请号:EP92920559.9
申请日:1992-09-29
IPC分类号: C07C395/00 , C07C391/00
CPC分类号: C07C395/00 , C07C391/00
摘要: A method of preparation of dialkyl tellurium, dialkyl selenium, dialkyl ditelluride and dialkyl diselenide is disclosed. The method comprises the reaction of the sodium calcogenide with an alkyl halide characterised by said reaction taking place in a two-phase system, and by the addition of a phase-transfer catalyst.
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公开(公告)号:EP0471723B1
公开(公告)日:1996-01-31
申请号:EP90907199.5
申请日:1990-05-02
CPC分类号: G06F15/8046 , G06F7/5443 , G06F17/16 , G06F2207/3892
摘要: A digital processor (10) for two's complement computations incorporates an array (12*) of multiplier cells (12) each having the one-bit gated full adder logic function. The array (12*) has nearest-neighbour connections (16, 18, 20) containing clock-activated latches (42, 44, 50) for bit propagation. On each clock cycle, the cells (12) receive input data, carry and cumulative sum bits. Each cell (12) adds the carry and cumulative sum bits to the product of the data bit and a respective coefficient digit associated with the relevant cell (12). Data bits pass along array rows and sum bits accumulate in cascade down array columns. Carry bits are recirculated. Each coefficient digit is expressed as a sign bit and at least one magnitude bit consisting of or including a level bit. Each cell (12) includes multiplicative gating means (58, 62) responsive to the sign and level bits, and carry feedback means (60, 66) responsive to a least significant data bit flag to substitute the sign bit for a carry feedback bit. Each coefficient digit may include an additional magnitude bit expressed as a shift bit and employed to select multiplicand data bit significance, the gating means (58, 62) being responsive to flag bits to eliminate unwanted sign extension bit products. The processor (10 or 200) may include accumulating means (14 or 214) incorporating gates (90, 98 or 292, 298, 336) responsive to flag bits and arranged to eliminate unwanted result sign extension bits.
摘要翻译: 用于二进制补码计算的数字处理器(10)包括每个具有一位门控全加器逻辑功能的乘法器单元(12)的阵列(12 *)。 阵列(12 *)具有包含用于比特传播的时钟激活锁存器(42,44,50)的最近邻居连接(16,18,20)。 在每个时钟周期中,单元(12)接收输入数据,进位和累加和位。 每个单元(12)将进位和累加和位添加到数据位和与相关单元(12)相关联的相应系数位的乘积。 数据位沿阵列行传递,总和位在级联向下阵列列中累加。 进位是循环的。 每个系数数字被表示为一个符号位和至少一个由一个电平位组成或包括一个电平位的幅度位。 每个单元(12)包括响应于符号和电平位的乘法门控装置(58,62),并且携带响应于最低有效数据位标志的反馈装置(60,66)以将符号位替换为进位反馈位。 每个系数数字可以包括表示为移位位并用于选择被乘数据位重要性的附加幅度位,门控装置(58,62)响应于标志位来消除不想要的符号扩展位产品。 处理器(10或200)可以包括累加装置(14或214),该装置响应于标志位而并入门(90,98或292,298,336)并且被安排成消除不想要的结果符号扩展位。
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公开(公告)号:EP0693099A1
公开(公告)日:1996-01-24
申请号:EP94912011.0
申请日:1994-03-30
CPC分类号: C09K19/3842 , C09K19/3852 , Y10T428/1005
摘要: A liquid crystal polymer or copolymer is disclosed of general structure (A) wherein X = CN, COR, CO2R, CHO, CF3; R = branched chain, straight chain or chiral alkyl; (a) = any suitable mesogenic group; Z = single covalent bond, oxygen, sulphur, CO2 or OCO; n = 1 to 20; Y = H, OH, F, OCH3; m = 3 to 200; P = a single covalent bond, oxygen, or OCO.
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公开(公告)号:EP0644787B1
公开(公告)日:1996-01-03
申请号:EP93913307.0
申请日:1993-06-07
发明人: BRIDGES, Peter Clive
CPC分类号: A62B18/08 , A62B18/025
摘要: A pressure breathing mask, particularly an aircrew oxygen mask, includes an exoskeleton (10), a flexible facepiece (11) with an oxygen delivery connection (15), an inflatable bladder (20) positioned between the exoskeleton (10) and a rigid intermediate member (30), the intermediate member (30) bearing on the facepiece (11), and means (21, 23) for automatically inflating the bladder (20) when oxygen is delivered under pressure to the mask.
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公开(公告)号:EP0650605A1
公开(公告)日:1995-05-03
申请号:EP94904902.0
申请日:1993-07-08
CPC分类号: G02B27/0176 , G02B5/10 , G02B5/18 , G02B27/017 , G02B27/0172 , G02B2027/0132 , G02B2027/0138 , G02B2027/015 , G02B2027/0152 , G02B2027/0156 , G02B2027/0161 , G02B2027/0187 , G02B2027/0198
摘要: An optical system mounted on a helmet (29) for superimposing a collimated image of a display object on the external view of the helmet's wearer comprising: display object means (1a, 1b) mounted on the helmet for generating a display; a concave collimator (6a, 6b) for collimating light from said display; an optical relay unit (3a, 3b) located in the forehead region for generating an intermediate image of said display in the focal plane of the collimator; a reflecting surface (4a, 4b) for reflecting display light from the relay unit towards the collimator; and an inclined combiner (5) essentially transparent to incoming display light and to the wearer's external view, for reflecting display light reflected from the collimator towards the viewer's eye.
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公开(公告)号:EP0644787A1
公开(公告)日:1995-03-29
申请号:EP93913307.0
申请日:1993-06-07
发明人: BRIDGES, Peter Clive
IPC分类号: A62B18
CPC分类号: A62B18/08 , A62B18/025
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