公开(公告)号：EP2217356B1

公开(公告)日：2018-10-17

申请号：EP08843483.2

申请日：2008-11-03

申请人： Biocius Life Sciences, Inc.

发明人： OZBAL, Can, C. ,  JONAS, Maxine ,  LAMARR, William, A. ,  VERNIKOV, Eduard

IPC分类号： G01N30/72 ,  B01D59/44

CPC分类号： H01J49/0431 ,  B01D15/305 ,  B01D15/34 ,  B01D15/361 ,  G01N1/405 ,  G01N30/02 ,  G01N30/24 ,  G01N30/7266 ,  G01N2030/207 ,  G01N2030/8411 ,  B01D15/36

摘要： One embodiment of the invention provides a sample injection system including a vacuum source, a conduit in communication with the vacuum source, a fluid sensor configured to detect the presence of the fluid in the conduit, a sample loop in communication with the conduit; and a sipper in communication with the sample loop.

公开(公告)号：EP3112858B1

公开(公告)日：2018-05-23

申请号：EP15754822.3

申请日：2015-02-23

申请人： Showa Denko K.K.

发明人： NAKAJIMA, Naoya ,  OKADA, Yoshiji ,  KONDO, Hideyuki

IPC分类号： G01N30/88 ,  G01N30/02 ,  G01N30/60 ,  G01N33/02 ,  B01D15/08 ,  B01D15/30 ,  B01J20/24 ,  B01J20/281 ,  B01J20/291 ,  B01J20/34

CPC分类号： B01J20/24 ,  B01D15/305 ,  B01J20/265 ,  B01J20/286 ,  B01J20/289 ,  B01J20/291 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3251 ,  B01J20/34 ,  G01N30/48 ,  G01N30/482 ,  G01N30/60 ,  G01N30/88 ,  G01N33/02 ,  G01N2030/027

摘要： Apackingmaterial for liquidchromatography, which is excellent in durability, and a column for liquid chromatography, which is filled with the packing material, are provided. The packing material for liquid chromatography is characterized by comprising a hydrophilic resin containing a polyvinyl alcohol resin, to said hydrophilic resin an amino group represented by the formula (1) having been bonded through a spacer. In the formula (1), R 1 represents a hydrogen atom or an alkyl group having 1 to 4 carbon atoms, R 2 represents an alkyl group having 1 to 6 carbon atoms and having one or more hydroxyl groups, and represents a bonding position to the spacer.

公开(公告)号：EP3169431A4

公开(公告)日：2018-03-14

申请号：EP15821602

申请日：2015-07-17

申请人： AZYP LLC

发明人： ARMSTRONG DANIEL W ,  BREITBACH ZACHARY S

IPC分类号： B01J20/32 ,  B01D15/30 ,  B01J20/10 ,  B01J20/22 ,  B01J20/24 ,  B01J20/28 ,  B01J20/283 ,  B01J20/288 ,  B01J20/289 ,  B01J20/29

CPC分类号： B01D15/305 ,  B01J20/103 ,  B01J20/22 ,  B01J20/24 ,  B01J20/28004 ,  B01J20/28061 ,  B01J20/28078 ,  B01J20/28083 ,  B01J20/283 ,  B01J20/288 ,  B01J20/289 ,  B01J20/29 ,  B01J20/3204 ,  B01J20/3217 ,  B01J20/3219 ,  B01J20/3263 ,  B01J20/3285 ,  B01J20/3293 ,  B01J2220/46 ,  B01J2220/54

摘要： The present invention relates to superficially porous particles (SPPs), also called core-shell, porous shell or fused core particles, which are state-of-the-art support materials used in the production of HPLC columns. Hydrolytically stable, highly selective superficially porous particle (SPP) hydrophilic interaction liquid chromatographic (HILIC) stationary phases having higher efficiencies and shorter retention times than analogous stationary phases on fully porous particles (FPP) is provided.

公开(公告)号：EP3116645A1

公开(公告)日：2017-01-18

申请号：EP15761376.1

申请日：2015-02-18

申请人： GE Healthcare BioProcess R&D AB

发明人： MALOISEL, Jean-Luc ,  BUSSON, Karolina ,  LACKI, Karol ,  NOREN, Bjorn ,  SKOGLAR, Helena

IPC分类号： B01J20/32 ,  B01D15/32 ,  B01D15/34 ,  B01D15/38 ,  B01J20/281 ,  C08J7/00

CPC分类号： B01J20/3293 ,  B01D15/125 ,  B01D15/203 ,  B01D15/305 ,  B01D15/363 ,  B01J20/28019 ,  B01J20/286 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/327 ,  B01J20/3278 ,  C07K1/22 ,  C07K14/765 ,  C07K14/77 ,  C12N1/20

摘要： The invention discloses a separation matrix for purification of biological particles, comprising a plurality of particles having a porous core entity and a porous shell entity covering the core entity, wherein the core entity comprises at least 50 micromole/ml primary amines present on covalently attached ligands displaying at least two primary amines per ligand and the shell entity comprises less than 20 micromole/ml primary amines. The invention further discloses a method of purifying biological particles and a method of manufacturing a separation matrix.

摘要翻译： 本发明公开了一种用于纯化生物颗粒的分离基质，其包含多个具有多孔核心实体和覆盖核心实体的多孔壳体的颗粒，其中核心实体包含共价连接的配体上存在的至少50微摩尔/ 每个配体显示至少两个伯胺，壳体包含少于20微摩尔/ ml伯胺本发明还公开了一种纯化生物颗粒的方法和一种制备分离基质的方法。

公开(公告)号：EP2961759A1

公开(公告)日：2016-01-06

申请号：EP14756237.5

申请日：2014-02-26

申请人： Agency for Science, Technology and Research

发明人： GAGNON, Peter Stanley

IPC分类号： C07K1/14 ,  B01D15/36 ,  C07K1/30

CPC分类号： C07K1/165 ,  B01D15/305 ,  B01D15/363 ,  B01D15/3847 ,  C07K1/30 ,  C07K1/303 ,  B01D15/327 ,  B01D15/34

摘要： A method of purifying a desired protein from a preparation includes (a) providing the preparation in a form having less than about 5% of chromatin residing in an original production medium, (b) contacting the preparation with a nonionic organic polymer and a salt, a concentration of nonionic organic polymer being sufficient to precipitate the desired protein or cause its accretion on a hydrophilic surface, or maintain it in a precipitated state or accreted on the hydrophilic surface, the salt concentration being sufficient to produce greater than physiological conductivity, and (c) contacting the preparation with at least one electropositive surface, optionally in the presence of a salt concentration sufficient to produce greater than physiological conductivity, the desired protein does not substantially adsorb to the at least one electropositive surface while not preventing adsorption of acidic contaminants to the at least one electropositive surface.

公开(公告)号：EP2731708A1

公开(公告)日：2014-05-21

申请号：EP12733762.4

申请日：2012-07-12

申请人： InstrAction GmbH

发明人： SCHWARZ, Thomas ,  WELTER, Martin ,  ARENDT, Markus ,  DEGEL, Björn

IPC分类号： B01J20/283 ,  B01J20/285 ,  B01J20/286 ,  B01J20/10 ,  B01J20/28 ,  B01J20/32 ,  B01J20/26

CPC分类号： B01D15/3804 ,  B01D15/305 ,  B01D15/327 ,  B01D15/34 ,  B01D15/362 ,  B01D15/363 ,  B01D15/3828 ,  B01J20/103 ,  B01J20/261 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/282 ,  B01J20/283 ,  B01J20/285 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3225 ,  B01J20/3227 ,  B01J20/3282 ,  B01J20/3293

摘要： The present application pertains to a composite material for chromatographic applications and a method for the preparation of the composite material.

公开(公告)号：EP2295962A4

公开(公告)日：2012-01-11

申请号：EP09762265

申请日：2009-06-10

申请人： SUMITOMO BAKELITE CO

发明人： SHIMAOKA HIDEYUKI ,  ABE MIDORI

IPC分类号： G01N30/88 ,  B01D15/32 ,  C07H1/06 ,  G01N27/447 ,  G01N27/62 ,  G01N30/06 ,  G01N30/26 ,  G01N30/72 ,  G01N33/58

CPC分类号： G01N30/88 ,  B01D15/305 ,  B01D15/322 ,  G01N27/447 ,  G01N30/34 ,  G01N30/7233 ,  G01N33/66 ,  G01N2030/381 ,  G01N2030/8836

公开(公告)号：EP2217356A1

公开(公告)日：2010-08-18

申请号：EP08843483.2

申请日：2008-11-03

申请人： BioTrove, Inc.

发明人： OZBAL, Can, C. ,  JONAS, Maxine ,  LAMARR, William, A. ,  VERNIKOV, Eduard

IPC分类号： B01D59/44

CPC分类号： H01J49/0431 ,  B01D15/305 ,  B01D15/34 ,  B01D15/361 ,  G01N1/405 ,  G01N30/02 ,  G01N30/24 ,  G01N30/7266 ,  G01N2030/207 ,  G01N2030/8411 ,  B01D15/36

摘要： One embodiment of the invention provides a method of preparing an eluted sample containing salts or buffers from a liquid chromatography device for analysis by a mass spectrometry device. The method includes: continuously providing a non-polar solvent to the mass spectrometry device; receiving the eluted sample from the liquid chromatography device; flowing the eluted sample over a solid phase extraction column; flowing the non-polar solvent over the solid phase extraction column; and presenting non-polar solvent and the eluted sample to the mass spectrometry device.

公开(公告)号：EP2027163B1

公开(公告)日：2010-07-21

申请号：EP07725927.3

申请日：2007-06-08

申请人： Merck Patent GmbH

发明人： IRGUM, Knut ,  LIME, Fredrik

IPC分类号： C08F2/48 ,  C08F12/36 ,  B01J20/26 ,  C08F212/06 ,  C08F220/02 ,  C08F2/06

CPC分类号： C08F2/48 ,  B01D15/305 ,  B01D15/327 ,  B01D15/361 ,  B01J20/26 ,  B01J20/261 ,  B01J20/265 ,  B01J20/267 ,  B01J20/28004 ,  B01J20/28019 ,  B01J20/285 ,  C08F212/08 ,  C08F212/14 ,  C08F212/36 ,  C08F220/06 ,  C08F220/26 ,  C08F220/30 ,  C08F2220/325 ,  Y10T428/2982

摘要： The invention relates to a polymeric material comprising crosslinked spherical particles that are obtained by photoinitiated precipitation polymerization of crosslinking monomer and optionally a further monomer in the presence of an initiator and a solvent. It also relates to a method for producing the material and a chromatography column containing the material.

公开(公告)号：EP0990258A1

公开(公告)日：2000-04-05

申请号：EP98935479.0

申请日：1998-06-19

申请人： Ciphergen Biosystems, Inc.

发明人： HUTCHENS, T., William ,  YIP, Tai-Tung

IPC分类号： H01J49/04

CPC分类号： C40B30/04 ,  B01D15/1878 ,  B01D15/305 ,  B01D15/3809 ,  B01J20/281 ,  B01J20/286 ,  B01J20/3204 ,  B01J20/3212 ,  B01J20/3219 ,  B01J20/3248 ,  B01J20/3253 ,  B01J20/3265 ,  B01J20/3274 ,  B01J20/3289 ,  B01J2219/00605 ,  B01J2219/0061 ,  B01J2219/00612 ,  B01J2219/00617 ,  B01J2219/00619 ,  B01J2219/00621 ,  B01J2219/00626 ,  B01J2219/00628 ,  B01J2219/0063 ,  B01J2219/00637 ,  B01J2219/00641 ,  B01J2219/00644 ,  B01J2219/00659 ,  B01J2219/00702 ,  B01J2219/00725 ,  B01J2220/54 ,  C12Q1/00 ,  C40B40/10 ,  G01N30/00 ,  G01N30/02 ,  G01N30/461 ,  G01N30/463 ,  G01N30/466 ,  G01N30/48 ,  G01N30/482 ,  G01N30/90 ,  G01N30/95 ,  G01N33/531 ,  G01N33/538 ,  G01N33/543 ,  G01N33/54306 ,  G01N33/54353 ,  G01N33/54373 ,  G01N33/54393 ,  G01N33/566 ,  G01N33/6803 ,  G01N33/6845 ,  G01N33/6851 ,  G01N2030/008 ,  G01N2030/009 ,  Y02A90/26 ,  Y10S422/906 ,  Y10S435/803 ,  Y10S435/81 ,  Y10S435/814 ,  Y10S435/815 ,  Y10S435/97 ,  Y10S435/973 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S436/809 ,  Y10S436/811 ,  Y10S436/813 ,  Y10S436/824 ,  Y10S436/825 ,  Y10T436/24 ,  Y10T436/25 ,  Y10T436/25125 ,  Y10T436/2525 ,  Y10T436/25375 ,  Y10T436/255 ,  B01D15/363 ,  B01D15/322 ,  B01D15/327 ,  B01D15/3804 ,  B01D15/362 ,  B01D15/38 ,  B01D15/26

摘要： This is invention is directed to methods of identifying analytes that are differentially present between two samples. The methods involve determining retention data by desorption spectrometry for analytes in different samples using the same selectivity conditions, comparing the data, and identifying analytes that are differentially retained.

摘要翻译： 本发明提供了用于分离样品中分析物的滞留层析方法。 该方法包括在多种不同的选择性条件下将分析物吸附到基质上，并通过解吸光谱法检测保留在基质上的分析物。 这些方法在生物学和药理学中很有用，包括临床诊断和药物发现。