公开(公告)号：EP2089413A4

公开(公告)日：2009-10-28

申请号：EP02759066

申请日：2002-02-21

申请人： AMNIS CORP

发明人： ORTYN WILLIAM ,  BASIJI DAVID

IPC分类号： G01N21/64 ,  C07H21/04 ,  C12M1/34 ,  C12Q1/68 ,  G01J3/44 ,  G01N15/14 ,  G01N27/447

CPC分类号： G01N27/44726 ,  C12Q1/6841 ,  G01J3/2803 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6456 ,  G01N2015/1472 ,  G01N2021/6421 ,  G01N2021/6441 ,  C12Q2565/102 ,  C12Q2537/143

公开(公告)号：EP1257794A4

公开(公告)日：2006-12-13

申请号：EP00973466

申请日：2000-10-12

申请人： AMNIS CORP

发明人： BASIJI DAVID ,  ORTYN WILLIAM

IPC分类号： C12Q1/02 ,  G01J3/28 ,  G01N15/02 ,  G01N15/06 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G01N33/48 ,  G02B7/28 ,  G02B27/00 ,  G02B27/14 ,  G06K9/00 ,  G06T1/00 ,  G01J3/18 ,  G01J3/30

CPC分类号： G06K9/00127 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/147 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/85 ,  G01N2015/0294 ,  G01N2015/0693 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G02B7/28 ,  G02B27/145 ,  G02B27/148

摘要： Light (30) from an object (24) such as a cell moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light (30) can be emitted from a luminous object (24) or can be light from a light source that has been scattered by the object or can be a fluorescent emission by one or more FISH probes, frequently used to detect substances within cells. Further, light that is absorbed or reflected by the object can also be used to produce images for determining specific characteristics of the object. The movement of the object matches the rate at which a signal is read from the TDI detector. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and spectrally dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：EP1330650A4

公开(公告)日：2008-03-19

申请号：EP01979992

申请日：2001-10-12

申请人： AMNIS CORP

发明人： FROST KEITH ,  BASIJI DAVID ,  BAUER RICHARD ,  FINCH ROSALYNDE ,  ORTYN WILLIAM ,  PERRY DAVID

IPC分类号： G01N21/64 ,  G01N33/543 ,  B01J19/00 ,  C07B61/00 ,  C07H21/04 ,  C12N15/09 ,  C12Q1/68 ,  G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/05 ,  G01N33/48 ,  G01N33/483 ,  G01N33/50 ,  G01N33/53 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

CPC分类号： G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C12Q1/6816 ,  C12Q1/6869 ,  C12Q2600/156 ,  C40B20/04 ,  C40B60/00 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6428 ,  G01N21/6456 ,  G01N2015/0294 ,  G01N2015/1018 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G01N2021/6421 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2565/626 ,  C12Q2563/149 ,  C12Q2563/107

摘要： Combinatorially-synthesized deoxyribonucleic acid (DNA) oligonucleotides attached to encoded beads that are hybridized to amplified and labeled genomic DNA or ribonucleic acid (RNA) are analyzed using a flow imaging system. Oligonucleotides and corresponding reporters are bound to the surfaces of a plurality of small beads such that different beads bear different oligo sequences. Each bead bears a unique optical signature comprising a predefined number of unique reporters, where each reporter comprises a predefined combination of different fluorochromes. The composite spectral signature in turn identifies the unique nucleotide sequence of its attached oligo chains. This optical signature is rapidly decoded using an imaging system to discriminate the different reporters attached to each bead in a flow in regard to color and spatial position on the bead (see fig. 15).

公开(公告)号：EP1317852A4

公开(公告)日：2009-07-22

申请号：EP01970583

申请日：2001-08-24

申请人： AMNIS CORP

发明人： BASIJI DAVID ,  ORTYN WILLIAM

IPC分类号： G01N21/64 ,  G01N15/00 ,  G01N15/02 ,  G01N15/14 ,  G01N21/27 ,  G06K9/00 ,  H04N5/232

CPC分类号： G06K9/00127 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/0232 ,  G01J3/14 ,  G01J3/2823 ,  G01J3/2889 ,  G01N15/14 ,  G01N21/6458 ,  G01N2015/1497

摘要： Light from an object (152) moving through an imaging system (154) is collected, dispersed, and imaged onto a time delay integration (TDI) detector (148) that is inclined relative to an axis of motion of the object, producing a pixilated output signal. In one embodiment, the movement of the image object over the TDI detector (148) is asynchronous with the movement of the output signal producing an output signal that is a composite of the image of the object at varying focal point along the focal plane. In another embodiment, light from the object is periodically incident on the inclined TDI, producing a plurality of spaced apart images and corresponding output signals that propagate across the TDI detector (148). The inclined plane enables images of FISH probes or other components within an object to be produced at different focal point, so that the 3D spatial relationship between the FISH probes or components can be resolved.

公开(公告)号：EP1334338A4

公开(公告)日：2008-03-19

申请号：EP01274071

申请日：2001-10-12

申请人： AMNIS CORP

发明人： ORTYN WILLIAM ,  BASIJI DAVID

IPC分类号： G01N15/14 ,  G01N33/483 ,  C07B61/00 ,  C07K1/04 ,  C12Q1/68 ,  G01J3/02 ,  G01J3/28 ,  G01J3/36 ,  G01J3/44 ,  G01J3/51 ,  G01N15/02 ,  G01N21/05 ,  G01N21/17 ,  G01N21/27 ,  G01N21/64 ,  G02B7/28 ,  G02B21/00 ,  G02B27/00 ,  G02B27/14

CPC分类号： G02B27/148 ,  B01J2219/00274 ,  C07B2200/11 ,  C07K1/047 ,  C12Q1/6816 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/021 ,  G01J3/2803 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1434 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2015/0294 ,  G01N2015/1443 ,  G01N2015/1472 ,  G01N2015/1479 ,  G01N2021/058 ,  G02B7/28 ,  G02B21/00 ,  G02B27/0012 ,  G02B27/1013 ,  G02B27/126 ,  G02B27/144 ,  G02B27/145 ,  C12Q2563/179 ,  C12Q2563/107

摘要： Light from an object such as a cell moving through an imaging system is collected and dispersed so that it is imaged onto a plurality of separate detectors (321-325). The light is spectrally dispersed by a plurality of spaced-apart dichroic reflectors (301-304), each detector (321-325) receiving light from a different one of the dichroic reflectors (301-304). Each dichroic filter (301-304) reflects light of a different predefined color, passing light of other colors. The output signal from each detector (321-325) is indicative of a different characteristic of the object. In one configuration, each detector (321-325) is provided with a separate imaging lens (311-315). In another configuration, the detectors (321-325) are spaced at varying distances from the corresponding dichroic reflectors (301-304), so that separate imaging lenses are not required.

公开(公告)号：EP1844426A4

公开(公告)日：2016-09-07

申请号：EP06720094

申请日：2006-02-01

申请人： AMNIS CORP

发明人： ORTYN WILLIAM ,  BASIJI DAVID ,  GEORGE THADDEOUS ,  MORRISSEY PHILIP ,  HALL BRIAN ,  PERRY DAVID ,  ZIMMERMAN CATHLEEN

IPC分类号： G06K9/00

CPC分类号： G06K9/00127 ,  G01N15/147 ,  G01N15/1475 ,  G01N2015/1497

摘要： Multimodal/multispectral images of a population of cells are simultaneously collected. Photometric and/or morphometric features identifiable in the images are used to separate the population of cells into a plurality of subpopulations. Where the population of cells includes diseased cells and healthy cells, the images can be separated into a healthy subpopulation, and a diseased subpopulation. Where the population of cells does not include diseased cells, one or more ratios of different cell types in patients not having a disease condition can be compared to the corresponding ratios in patients having the disease condition, enabling the disease condition to be detected. For example, blood cells can be separated into different types based on their images, and an increase in the number of lymphocytes, a phenomenon associated with chronic lymphocytic leukemia, can readily be detected.

公开(公告)号：EP1886139A4

公开(公告)日：2008-07-16

申请号：EP06758550

申请日：2006-04-20

申请人： AMNIS CORP

发明人： ORTYN WILLIAM ,  BASIJI DAVID ,  LYNCH DAVID

IPC分类号： G01N33/53

CPC分类号： G01N33/505 ,  G01J3/2889 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/53 ,  G01N21/6428 ,  G01N21/6456 ,  G01N21/6458 ,  G01N33/5005 ,  G01N2015/1006 ,  G01N2015/1497 ,  G01N2021/6421 ,  G01N2021/6441 ,  G06K9/00127

摘要： Aspects of the present invention encompass the collection of multispectral images from a population of objects, and the analysis of the collected images to measure at least one characteristic of the population, using photometric and/or morphometric features identifiable in the collection of images. In an exemplary application, the objects are biological cells. In a particularly preferred, but not limiting implementation, the plurality of images for each individual object are collected simultaneously. In an empirical study, the characteristic being measured involves the synapse between conjugated cells. The conjugated cells may represent a subpopulation of the overall population of objects that were imaged. In a particularly preferred, yet not limiting embodiment, the present invention enables the quantization of the redistribution of cellular molecules due to the conjugation of different biological cells. Significantly, such quantization is not feasible with standard microscopy and flow cytometry.

摘要翻译： 本发明的各方面包括从物体群收集多光谱图像，并且采集所收集图像的分析以使用图像集合中可识别的光度和/或形态学特征来测量群体的至少一个特征。 在示例性应用中，物体是生物细胞。 在特别优选的但非限制性的实现中，同时收集每个单独对象的多个图像。 在实证研究中，被测量的特征涉及共轭细胞之间的突触。 结合的细胞可以代表成像的整个对象群体的亚群。 在一个特别优选的但非限制性的实施方案中，本发明能够量化由于不同生物细胞的缀合而导致的细胞分子的重新分布。 重要的是，这种量化不适用于标准显微镜和流式细胞术。

公开(公告)号：EP1272822A4

公开(公告)日：2008-03-12

申请号：EP00973491

申请日：2000-10-12

申请人： AMNIS CORP

发明人： BASIJI DAVID ,  ORTYN WILLIAM

IPC分类号： G01J3/28 ,  G01N15/02 ,  G01N15/10 ,  G01N15/14 ,  G01N21/64 ,  G01N21/85 ,  G02B7/28 ,  G02B27/14 ,  G01J3/12 ,  G01J9/00

CPC分类号： G01N21/6456 ,  G01J3/2803 ,  G01J3/2823 ,  G01N15/147 ,  G01N21/6428 ,  G01N21/85 ,  G01N2015/0294 ,  G01N2015/1006 ,  G01N2015/1472 ,  G01N2015/1486 ,  G02B7/28 ,  G02B27/145 ,  G02B27/148

摘要： Light from an object (24), such as a cell, moving through an imaging system (20) is collected and dispersed so that it can be imaged onto a time delay and integration (TDI) detector (44). The light can be emitted from a luminous object or can be light from a light source that has been scattered or not absorbed by the object or can include a light emission by one or more probes within or on the object. Multiple objects passing through the imaging system (20) can be imaged, producing both scatter images and dispersed images at different locations on one or more TDI detectors (44).

公开(公告)号：EP1328894A4

公开(公告)日：2005-11-09

申请号：EP01970578

申请日：2001-08-24

申请人： AMNIS CORP

发明人： BASIJI DAVID ,  ORTYN WILLIAM

IPC分类号： G01P3/36 ,  G01N15/10 ,  G01N15/14 ,  G01N33/48 ,  G01N33/483 ,  G01P5/26 ,  G02B27/00 ,  G06T7/20

CPC分类号： G02B27/0012 ,  G01N15/1012 ,  G01N15/1459 ,  G01N15/147 ,  G01N2015/1018 ,  G01N2015/1025 ,  G01N2015/1075 ,  G01N2015/144 ,  G01N2015/1443 ,  G01N2015/1447 ,  G01N2015/1452 ,  G01N2015/1497 ,  G06T7/20

摘要： Frequency domain velocity measurements and time domain velocity measurements are made using light from cells or other objects (18a). An optical grating (46) is used to modulate the light from an object so that it has a frequency proportional to the velocity of the object. Depending upon the embodiment, the pitch of the optical grating is uniform or varying. The modulated light is detected and various signal processing techniques, such as a Fast Fourier Transform function or processing in the time domain, are used to determinate an indication of the velocity of the object. Preferably, the indication of the velocity is applied in producing a timing signal employed for synchronization of an image of the object and an detector signal in an optical analysis system that uses a time delay integration detector to determine characteristics of the object in response to light from the object.