公开(公告)号：EP0461177B1

公开(公告)日：1999-01-27

申请号：EP90904479.4

申请日：1990-03-02

申请人： The University of Virginia Patent Foundation

发明人： HERR, John, C. ,  WRIGHT, Richard, M.

IPC分类号： A61K39/00 ,  A01N1/02 ,  G01N33/53 ,  C12P21/06 ,  C07K14/00 ,  C07K1/14

CPC分类号： C07K16/28 ,  A61K38/00 ,  A61K39/00 ,  C07K14/47 ,  C07K14/575 ,  C07K14/705 ,  C07K16/18 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/50 ,  Y10S424/811 ,  Y10S530/852

摘要： A substantially purified intra-acrosomal human sperm antigen useful in a contraceptive vaccine is disclosed herein. The antigen remains associated with human sperm after the acrosome reaction. In particular, it remains associated with the inner and outer acrosomal membranes. Modified antigens and fragments thereof prepared by protein modification techniques are also disclosed as well as methods for purifying and using the antigens. Also disclosed are monoclonal and polyclonal antibodies to the antigen and methods of making and using such antibodies. Methods of use include purification of the antigen or use in various diagnostic techniques. Also disclosed are cDNA, expression vectors, and transformed microorganisms that produce the antigen.

公开(公告)号：EP0876610A1

公开(公告)日：1998-11-11

申请号：EP97901914.0

申请日：1997-01-06

申请人： THE PENN STATE RESEARCH FOUNDATION

发明人： HAMMERSTEDT, Roy, H. ,  CRAMER, Palmer, G. ,  BARBATO, Guy, F.

IPC分类号： G01N33 ,  A61K38 ,  A61P15 ,  C07K7 ,  C07K14

CPC分类号： C07K14/47 ,  A61K38/00 ,  C07K14/705 ,  Y10S530/827 ,  Y10S530/85 ,  Y10S530/851 ,  Y10S530/852 ,  Y10S530/853

摘要： A purified polypeptide which provides for initial binding of sperm to oocyte investments and has an active amino acid sequence of SEQ ID NO:12 (Cys-Gln-Ser-Leu-Gln-Glu-Tyr-Leu-Ala-Glu-Gln-Asn-Gln-Arg-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Val-Asp-Leu-Ala-Arg-Val-Val-Ala-Pro-Phe-Met-Ser-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Arg-Pro-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn-Glu-Asp-Val-Cys); or SEQ ID NO:13 (Cys-Glu-Ser-Leu-Gln-Lys-His-Leu-Ala-Glu-Leu-Asn-His-Gln-Lys-Gln-Leu-Glu-Ser-Asn-Lys-Ile-Pro-Glu-Leu-Asp-Met-Thr-Glu-Val-Val-Ala-Pro-Phe-Met-Ala-Asn-Ile-Pro-Leu-Leu-Leu-Tyr-Pro-Gln-Asp-Gly-Pro-Arg-Ser-Lys-Pro-Gln-Pro-Lys-Asp-Asn-Gly-Asp-Val-Cys); or the shorter but biologically active SEQ ID NO:1 and SEQ ID NO:9 (Tyr-Pro-Gln-Asp-Arg-X-Arg-Ser-Gln-Pro-Gln-Pro-Lys-Ala-Asn, where X is Thr or Pro). The polypeptide is useful in improving sperm-egg binding by fresh sperm, restoring sperm-egg binding following a cryopreservation cycle, enhancing fertilizing potential, and producing antibodies to determine fertilizing potential of sperm, determining sperm-binding sites on egg investments, and contraception.

公开(公告)号：EP0894498B1

公开(公告)日：2006-05-17

申请号：EP98112150.2

申请日：1998-07-01

申请人： VICAM, L.P.

发明人： Benjamin, Thomas L. ,  Kohn, Barbara ,  Basker, Christopher J. ,  George, Susan ,  Livingston, David

IPC分类号： A61K35/52 ,  G01N33/483 ,  C12N5/06

CPC分类号： C12N5/0612 ,  G01N33/54326 ,  G01N33/56966 ,  Y10S530/852

公开(公告)号：EP1021717B1

公开(公告)日：2002-04-10

申请号：EP97912688.5

申请日：1997-10-03

申请人： EMBREX INC.

发明人： PHELPS, Patricia, V.

IPC分类号： G01N33/50 ,  G01N33/74 ,  A01K67/00

CPC分类号： G01N33/5088 ,  A01K43/00 ,  G01N33/50 ,  G01N33/743 ,  G01N2333/465 ,  Y10S530/827 ,  Y10S530/852 ,  Y10S530/853 ,  Y10T436/10 ,  Y10T436/143333

摘要： A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

公开(公告)号：EP0386166B1

公开(公告)日：1997-01-08

申请号：EP89904709.6

申请日：1989-03-24

申请人： INSTITUTE FOR HUMAN GENETICS AND BIOCHEMISTRY ,  RODMAN, Toby C.

发明人： RODMAN, Toby, C.

IPC分类号： C07K16/06 ,  C07K16/18 ,  G01N33/569 ,  G01N33/68

CPC分类号： C07K16/18 ,  G01N33/6875 ,  G01N33/689 ,  G01N2800/52 ,  Y10S435/974 ,  Y10S435/975 ,  Y10S436/808 ,  Y10S530/852 ,  Y10S530/863

摘要： The present invention is directed to protamine-reactive, IgM antibodies which may be non-induced, natural antibodies. In a specific embodiment, the invention relates to a low affinity binding subset of protamine-reactive serum IgM antibodies which recognize a sequence comprising four arginyl residues, including a triplet, within a six amino acid residue sequence, methods and diagnostic kits thereof. This low affinity subset is detectable in sera of normal individuals and HIV-infected individuals who subsequently exhibit a significant period of latency, but are absent or deficient in sera of individuals diagnosed with AIDS and sera of HIV-infected individuals, who though asymptomatic at the time of sampling, proceed to AIDS within a relatively short time.

公开(公告)号：EP0410816A3

公开(公告)日：1992-10-07

申请号：EP90308341.8

申请日：1990-07-30

申请人： WISCONSIN ALUMNI RESEARCH FOUNDATION

发明人： Ax, Roy L. ,  Miller, David J. ,  Winer, Martin A.

IPC分类号： G01N33/68 ,  A61K35/52

CPC分类号： G01N33/689 ,  A61K38/00 ,  G01N33/86 ,  G01N2400/40 ,  G01N2800/367 ,  Y10S436/906 ,  Y10S530/852

摘要： A method is disclosed relating to the isolation and purification of certain heparin-binding protein compositions in the seminal plasma of male mammals. The isolated proteins are useful in fertility studies and in enhancing the acrosomal reaction, capacitation and subsequent fertility of sperm cells.

公开(公告)号：EP0461177A1

公开(公告)日：1991-12-18

申请号：EP90904479.0

申请日：1990-03-02

申请人： CENTER FOR INNOVATIVE TECHNOLOGY

发明人： HERR, John, C. ,  WRIGHT, Richard, M.

IPC分类号： A61K38 ,  A61K39 ,  C07H21 ,  C07K1 ,  C07K14 ,  C07K16 ,  C07K19 ,  C12N1 ,  C12N5 ,  C12N15 ,  C12P21 ,  G01N33 ,  C12R1

CPC分类号： C07K16/28 ,  A61K38/00 ,  A61K39/00 ,  C07K14/47 ,  C07K14/575 ,  C07K14/705 ,  C07K16/18 ,  C07K19/00 ,  C07K2319/00 ,  C07K2319/23 ,  C07K2319/40 ,  C07K2319/50 ,  Y10S424/811 ,  Y10S530/852

摘要： Antigène du sperme humain intra-acrosomal largement purifié, destiné à un vaccin contraceptif. Cet antigène reste lié avec le sperme humain même après la réaction acrosomale. Il reste en particulier lié avec les membranes acrosomales interne et externe. L'invention porte en outre sur des antigènes et fragments d'antigène modifiés, préparés grâce à des techniques de modification de protéines, et sur des méthodes de purification et d'utilisation des antigènes, ainsi que sur des anticorps monoclonaux et polyclonaux de cet antigène et sur des méthodes pour les préparer et pour les utiliser, notamment en les purifiant ou pour diverses techniques de diagnostic. L'invention porte enfin sur un ADN complémentaire, sur des vecteurs d'expression et sur des microorganismes transformés qui produisent cet antigène.

公开(公告)号：EP1133313A2

公开(公告)日：2001-09-19

申请号：EP99972927.0

申请日：1999-12-01

申请人： KING'S COLLEGE LONDON ,  ST.GEORGE'S HOSPITAL MEDICAL SCHOOL

发明人： FRASER, Lynn, Repsis ,  PONDEL, Marc, Dean

IPC分类号： A61K38/23 ,  A61K35/52 ,  C12N5/00 ,  G01N33/58

CPC分类号： C12N5/061 ,  A61K35/52 ,  A61K38/23 ,  C12N2500/14 ,  C12N2517/10 ,  Y10S435/806 ,  Y10S530/852

摘要： The hormone calcitonin promotes fertilizing ability in mammalian sperm and is useful for treatment of conditions of low fertility in humans and animals. Human, porcine or salmon calcitonin may be formulated in a pharmaceutical composition comprising a pharmaceutically acceptable carrier for topical application, e.g. as a cream or jelly containing up to 100 ng/ml or more of salmon calcitonin or up to 2 mug/ml or more of human calcitonin. Calcitonin may also be administered parenterally, orally, or nasally. For improving in vitro fertilization or artificial insemination methods calcitonin is added to sperm prior to use e.g. salmon calcitonin present at a concentration of from 5 to 50 ng/ml or human calcitonin at a concentration of from 20 to 200 ng/ml in the sperm preparation. Calcitonin levels in seminal plasma can also be used to diagnose infertility.

公开(公告)号：EP1083925A1

公开(公告)日：2001-03-21

申请号：EP99920498.5

申请日：1999-05-13

申请人： Immucon Inc.

发明人： SULLIVAN, Robert ,  BERUBE, Bruno ,  LEGARE, Christine ,  GAUDREAULT, Christian

IPC分类号： A61K39/00 ,  C12Q1/68 ,  G01N33/68

CPC分类号： A61K39/0006 ,  A61K39/00 ,  C07K14/47 ,  Y10S530/806 ,  Y10S530/852

摘要： The present invention relates to an acrosomal sperm protein for use in immunocontraception of male and female subjects and as a marker for fertility. The present invention provides a method of immunocontraception of a male or female subject, which comprises administering an antigenic fragment of a P34 protein showing a high specificity for the P34 to the subject to elicit an immunocontraception response by the subject. An immune response against the peptide occurs without side effects. Probes and diagnosis kits for male or female infertility are also provided.

公开(公告)号：EP0517802A1

公开(公告)日：1992-12-16

申请号：EP91905665.0

申请日：1991-03-04

申请人： BRIGHAM & WOMEN'S HOSPITAL

发明人： ANDERSON, Deborah J. ,  JOHNSON, Peter M. ,  JACK, Richard, M.

IPC分类号： G01N33

CPC分类号： G01N33/56966 ,  G01N33/564 ,  G01N33/689 ,  G01N2800/367 ,  Y10S435/806 ,  Y10S436/821 ,  Y10S436/906 ,  Y10S530/852

摘要： Procédés de détection et d'isolement de spermatozoïdes ayant réagi avec l'acrosome et oocytes portant le récepteur du complément utilisant le composant du complément, fragments ou variantes de celui-ci, anticorps contre un récepteur du complément, ou anticorps contre le C3. Ces procédés ont une application dans l'évaluation de la fécondité, dans la préparation de spermatozoïdes ou d'oocytes pour la fécondation in vitro ou pour le transfert de gamètes dans la trompe de Fallope, dans la stimulation ou l'inhibition de la fécondation in vitro et in vivo, et dans le diagnostic et le traitement de l'infécondité.