摘要:
PROBLEM TO BE SOLVED: To provide production methods of arachidonic acid containing oils, substantially free of eicosapentaenoic acid, and to provide compositions containing oils containing arachidonic acid having a high concentration triglyceride form. SOLUTION: Filamentous fungus Mortierella alpina is cultivated by using conditions of producing triglyceride oil containing particularly much arachidonic acid, biomass is collected and the oil is extracted and recovered, and used as an additive for formula milk. COPYRIGHT: (C)2009,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To economically produce arachidonic acid and to provide a food formulated with the same. SOLUTION: Mortierella sect. schmuckeri microorganisms are cultured in fermentation medium, preferably containing a component of a complex nitrogen source. The arachidonic acid is important as a precursor of eicosanoid which is a nutrient for infant, and a food product which includes Mortierella sect. schmuckeri microorganisms or lipid isolated from such microorganisms to enhance the arachidonic acid content of the food product for infant is produced. COPYRIGHT: (C)2008,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To improve flavor, softness and/or consumer acceptability of poultry meat. SOLUTION: Low-level long chain ω-3 and/or ω-6HUFA originating from a microorganism source, an animal source or a genetically modified plant source is fed to domestic fowls such as broiler chickens, capons, turkeys, guinea fowls, quails, ducks and geese preferably in a latter stage of a production cycle to reinforce the meat. This can improve a nutritive value of the meat. COPYRIGHT: (C)2008,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To provide a single cell edible oil containing DHA (docosahexaenoic acid). SOLUTION: The single cell edible oil containing a triglyceride is produced by using a dinoflagellate, preferably Cryptecodinium cohnii as a source. DHA accounts for ≥20% of the oil. More preferably, the single cell edible oil has a triglyceride content of ≥70% and contains DHA accounting for ≥30% of the triglyceride. COPYRIGHT: (C)2007,JPO&INPIT
摘要:
PROBLEM TO BE SOLVED: To obtain a nutritional composition expected to decrease the cost of a marine product food cultured in a fish farm if enabling the supply of sufficient DHA to fry young fish to increase the survival ratio of the fry young fish. SOLUTION: This nutritional composition comprises a particulate substance prepared by drying slurry containing a polar lipid extract from a docosahexaenoic acid (DHA)-containing microorganism and an arachidonic acid (ARA)-containing microorganism, and containing a phospholipid having DHA residue and ARA residue. COPYRIGHT: (C)2006,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a process for extracting lipids from microorganisms without using a large amount of an arbitrary non-polar organic solvent, by which the lipids are extracted, recovered, isolated or obtained from the microorganisms. SOLUTION: This solvent-free extraction process is a method for extracting the lipids from the microorganisms without using a non-polar organic solvent as an extraction solvent. More concretely, the method for extracting the lipids from the microorganisms comprises dissolving cells until to obtain the substantially non-emulsified lipids, and then washing the obtained cell mixture with a water-soluble washing solution to remove water-soluble compounds and / or materials. COPYRIGHT: (C)2006,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a process for proliferating eukaryotic microorganisms producing lipids, in particular lipids containing polyenoic fatty acids, and to provide a method for producing the lipids from the eukaryotic microorganisms. SOLUTION: The process for proliferating, the eukaryotic microorganisms producing at least about 20% of eukaryotic microorganism biomass as lipids is provided, wherein the lipids preferably contain one or more polyenoic fatty acids, and a carbon source preferably a non-alcohol carbon source and restricted nutrient sources are added to a fermentation medium containing the eukaryotic microorganisms. The carbon source and the restricted nutrients are preferably added at a sufficient rate to increase biomass density of the fermentation medium at least at about 100 g/L. COPYRIGHT: (C)2006,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a method for producing dinoflagellate biomass containing a single cell oil for lean diet. SOLUTION: The method for producing dinoflagellate biomass containing the single cell oil for lean diet comprises following (a) to (f). (a) adding about 10 6 cell/ml (0.5-1.0 g dry weight per one liter) Crytecodinium cohnii in a fermenter containing a nutrient solution comprising artificial sea water of about 1/4 to 1/2 salinity, 1-8% glucose and 0.4-0.8% yeast extract, (b) cultivating C. cohnii at 15-34°C and pH at 5.0-9.0, (c) successively adding glucose and yeast extract gradually into the nutrient solution covering approximately 56 h, (d) further adding glucose into the nutrient solution covering about 16 h to induce oil production by C. cohnii, (e) agitating and aerating the fermenter to keep dissolved oxygen content at at least about 20% of air saturation level throughout cultivation and (f) recovering C. cohnii about 60 h to about 90 h thereafter. COPYRIGHT: (C)2004,JPO&NCIPI
摘要:
PROBLEM TO BE SOLVED: To provide a method for preparing isotope-labeled gultamine, asparagine or cysteine used in producing an isotope-labeled protein for NMR measurement, and to provide a prepared product. SOLUTION: This method for preparing the isotope-labeled gultamine or asparagine comprises (a) reacting isotope-labeled gultamic acid or aspartic acid with an N-protecting group to form an N-protected amino acid, (b) cyclizing the N-protected amino acid to form an oxazolidinone, (c) reacting the oxazolidinone with ammonia under an amide formation condition to form a cyclic amide derivative, (d) hydrolyzing the cyclic amide derivative to form an N-protected amino acid, and (e) removing the protecting group from the N-protected amino acid to form the isotope-labeled gultamine or asparagine. Further, the isotope-labeled cysteine is provided. COPYRIGHT: (C)2004,JPO
摘要:
PROBLEM TO BE SOLVED: To provide an isotope-labeled nutrient medium for producing an isotope-labeled protein useful for NMR measurement and a method for obtaining a sample for NMR spectroscopy to determine three-dimensional structure information on a protein. SOLUTION: The nutrient medium capable of supporting the proliferation of a mammal or insect cell culture contains all amino acids essential for proliferation of the cells, assimilatable carbohydrate sources, essential inorganic substances, and growth factors. The amino acids and the all other substrates used by the cells for protein synthesis are substantially labeled with 2 H, 13 C or both of 13 C and 15 N. COPYRIGHT: (C)2004,JPO