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    Methods Human periosteum culture
    71.
    发明专利
    Methods Human periosteum culture 有权
    空值

    公开(公告)号：JP5330996B2

    公开(公告)日：2013-10-30

    申请号：JP2009529083

    申请日：2008-08-22

    申请人： 国立大学法人 新潟大学

    发明人： 知之 川瀬 ,  一博 奥田

    IPC分类号： C12N5/071 ,  A61L27/00 ,  C12N5/077

    CPC分类号： A61L27/3604 ,  A61L27/3839 ,  A61L27/3895 ,  C12N5/0654 ,  C12N2500/84 ,  C12N2501/115 ,  C12N2501/15

    摘要： The present invention provides a method for forcingly culturing a piece of human periosteum tissue in a shorter culture period, the method including the steps of: (1) placing a periosteum piece dissected from a patient on a culture dish containing no culture solution; (2) dropping platelet-rich plasma collected from the patient onto the surface of the periosteum piece on the culture dish and coagulating the platelet-rich plasma so as to cover the surface of the periosteum piece; (3) adding a first culture medium to the culture dish and growing the culture; and (4) growing the culture in a second culture medium containing a basic fibroblast growth factor and no platelet-rich plasma, after the step (3).

    摘要翻译： 本发明提供了一种在较短的培养期间强制培养人骨膜组织的方法，所述方法包括以下步骤：（1）将患者解剖的骨膜片置于不含培养液的培养皿中; （2）将从患者收集的富含血小板的血浆从培养皿上的骨膜片的表面上滴下，使富含血小板的血浆凝结，以覆盖骨膜的表面; （3）向培养皿中加入第一培养基并培养培养物; 和（4）在步骤（3）之后，在含有碱性成纤维细胞生长因子并且不含富含血小板的血浆的第二培养基中培养培养物。

    Medicament for the repair and regeneration of the cell growth method as well as the organization
    73.
    发明专利
    Medicament for the repair and regeneration of the cell growth method as well as the organization 有权
    空值

    公开(公告)号：JP4936341B2

    公开(公告)日：2012-05-23

    申请号：JP2009532060

    申请日：2008-09-10

    申请人： 北海道公立大学法人 札幌医科大学

    发明人： 清博 宝金 ,  修 本望

    IPC分类号： C12N5/0775 ,  A61K35/12 ,  A61P1/16 ,  A61P1/18 ,  A61P3/00 ,  A61P3/06 ,  A61P3/08 ,  A61P3/10 ,  A61P9/00 ,  A61P9/10 ,  A61P13/08 ,  A61P13/12 ,  A61P25/00 ,  A61P25/28 ,  A61P43/00 ,  C12N5/07 ,  C12N5/074 ,  C12N5/0797

    CPC分类号： A61K35/28 ,  A61K35/12 ,  A61K35/16 ,  A61K2035/124 ,  C12N5/0663 ,  C12N2500/84 ,  C12N2501/91

    摘要： The present invention relates to a method for growing, rapidly and massively ex vivo, cells collected from a living subject to provide a safe and effective pharmaceutical preparation for biological tissue repair/regeneration. Specifically, the present invention relates to a method for growing cells in a sample collected from a living subject by culturing the cells in a medium containing allogeneic (including autogenic) serum. Preferably the allogeneic serum has been determined as being negative for a serum tumor marker and/or an infectious factors, and the amount of the anticoagulant (e.g., heparin, a heparin derivative, or a salt thereof) added to the collected sample is less than 5 U/mL with respect to the volume of the sample or the amount of the anticoagulant in the medium at the start of culture is less than 0.5 U/mL. The present invention further relates to use of the method.

    摘要翻译： 本发明涉及一种用于生长，快速和大量离体从生物体收集的细胞的方法，以提供用于生物组织修复/再生的安全有效的药物制剂。 具体而言，本发明涉及通过在含有同种异体（含自身）血清的培养基中培养细胞来培养从活体收集的样本中的细胞的方法。 优选地，同种异体血清已经被确定为对于血清肿瘤标志物和/或感染因子是阴性的，并且添加到所收集的样品中的抗凝血剂（例如肝素，肝素衍生物或其盐）的量小于 相对于样品体积为5U / mL或培养开始时培养基中抗凝血剂的量小于0.5U / mL。 本发明还涉及该方法的用途。