公开(公告)号：US6140089A

公开(公告)日：2000-10-31

申请号：US251004

申请日：1999-02-16

Applicant: Patrick Aebischer ,  Beth A. Zielinski

Inventor： Patrick Aebischer ,  Beth A. Zielinski

IPC: A61K35/12 ,  A61L27/20 ,  A61L27/38 ,  C12N5/077 ,  C12N5/0793 ,  C12N11/10 ,  C12N11/04 ,  C12N5/00

CPC classification number: C12N5/0012 ,  A61L27/20 ,  A61L27/38 ,  A61L27/3804 ,  C12N5/0618 ,  C12N5/0619 ,  A61K2035/128 ,  A61K35/12

Abstract: Encapsulated viable cells for implanting are prepared having cells dispersed in a particulate, essentially non cross-linked chitosan core matrix that is enclosed within a semipermeable membrane. The cells are entrapped between chitosan particles of the core matrix and there is essentially no interfacial cross-linking between the core matrix and the membrane. The core matrix provides a physical support for the cells such that the cells are evenly dispersed throughout the core matrix so as to allow their maintenance, growth, proliferation and differentiation. The encapsulated cells may be prepared by mixing viable cells with a solution of chitosan, encapsulating the resultant mixture in a thermoplastic semipermeable membrane, and causing the chitosan to precipitate such as by changing the pH to form the core matrix. Alternatively, the chitosan in solution is precipitated to form the core matrix containing cells, and the core matrix is encapsulated in a semipermeable membrane. Cells encapsulated include neurosecretory cell lines, .beta.-cell-derived cells lines, fibroblasts, myocytes and glial cells.

Abstract translation: 制备用于植入的包封的活细胞，其具有分散在颗粒状基本上非交联壳聚糖核心基质中的细胞，其被包封在半透膜内。 细胞被截留在核心基质的壳聚糖颗粒之间，并且核心基质和膜之间基本上没有界面交联。 核心基质为细胞提供物理支持，使得细胞均匀地分散在整个核心基质中，以便使其维持，生长，增殖和分化。 包封的细胞可以通过将活细胞与壳聚糖溶液混合，将所得混合物包封在热塑性半透膜中并使壳聚糖沉淀，例如通过改变pH以形成核心基质来制备。 或者，将溶液中的壳聚糖沉淀以形成含核心基质的细胞，并将核心基质包封在半透膜中。 包封的细胞包括神经分泌细胞系，β-细胞来源的细胞系，成纤维细胞，肌细胞和神经胶质细胞。

公开(公告)号：US5871985A

公开(公告)日：1999-02-16

申请号：US294149

申请日：1994-08-22

Applicant: Patrick Aebischer ,  Beth A. Zielinski

Inventor： Patrick Aebischer ,  Beth A. Zielinski

IPC: A61K35/12 ,  A61L27/20 ,  A61L27/38 ,  C12N5/077 ,  C12N5/0793 ,  C12N11/10 ,  C12N11/04 ,  C12N5/00

CPC classification number: C12N5/0012 ,  A61L27/20 ,  A61L27/38 ,  A61L27/3804 ,  C12N5/0618 ,  C12N5/0619 ,  A61K2035/128 ,  A61K35/12

Abstract: Vehicles containing cells for implanting in the tissue of an individual are prepared having cells dispersed in a particulate, essentially non cross-linked chitosan core matrix that is enclosed within a semipermeable membrane. The cells are entrapped between chitosan particles of the core matrix and there is essentially no interfacial cross-linking between the core matrix and the membrane. The core matrix provides a physical support for viable cells within the vehicle such that the cells are evenly dispersed throughout the core matrix so as to allow their maintenance, growth, proliferation and differentiation. The vehicle can be prepared by mixing viable cells with a solution of chitosan, encapsulating the resultant mixture in a semipermeable membrane and causing the chitosan to precipitate such as by changing the pH to form the core matrix. Alternatively, the chitosan is precipitated to form the core matrix containing cells and then the core matrix is encapsulated in a semipermeable membrane. Cells within the core matrix may be neurosecretory cell lines, .beta.-cell-derived cells lines, fibroblasts, myocytes and glial cells.

Abstract translation: 制备含有用于植入个体组织的细胞的载体，其具有分散在颗粒状基本上非交联壳聚糖核心基质中的细胞，所述壳聚糖核心基质被包封在半透膜内。 细胞被截留在核心基质的壳聚糖颗粒之间，并且核心基质和膜之间基本上没有界面交联。 核心基质为载体内的活细胞提供物理支持，使得细胞均匀分散在整个核心基质中，以使其维持，生长，增殖和分化。 载体可以通过将活细胞与壳聚糖溶液混合来制备，将所得混合物包封在半透膜中并使壳聚糖沉淀，例如通过改变pH以形成核心基质。 或者，将壳聚糖沉淀形成含核心基质的细胞，然后将核心基质包封在半透膜中。 核心基质内的细胞可以是神经分泌细胞系，β-细胞来源的细胞系，成纤维细胞，肌细胞和神经胶质细胞。