公开(公告)号：US5470974A

公开(公告)日：1995-11-28

申请号：US202664

申请日：1994-02-25

申请人： James Summerton ,  Dwight Weller ,  Eugene Stirchak

发明人： James Summerton ,  Dwight Weller ,  Eugene Stirchak

IPC分类号： A61K47/48 ,  C07H21/00 ,  C07K14/00 ,  C12Q1/68 ,  C07D413/04 ,  C07D413/12 ,  C07D473/26

CPC分类号： C07H21/00 ,  A61K47/48061 ,  A61K47/48092 ,  C07K14/003 ,  C12Q1/6813 ,  C12Q1/682 ,  B01J2219/0072

摘要： A composition of polymer molecules effective to bind, with substantially uniform binding affinity, to a single-stranded polynucleotide containing a target sequence of bases. The polymer molecules are composed of a sequence of base-pairing moieties effective to hydrogen bond to corresponding, complementary bases in the target sequence, under selected binding conditions, and a predominantly uncharged, achiral backbone supporting the base-pairing moieties at positions and in orientations which allow hydrogen bonding between the pairing moieties of the polymer and the corresponding complementary bases in the target sequence. The composition has diagnostic uses, in a solid-support assay system, and therapeutic uses involving inhibition or inactivation of target polynucleotides.

公开(公告)号：US5142047A

公开(公告)日：1992-08-25

申请号：US100033

申请日：1987-09-23

申请人： James Summerton ,  Dwight Weller ,  Eugene Stirchak

发明人： James Summerton ,  Dwight Weller ,  Eugene Stirchak

IPC分类号： G01N33/50 ,  A61K47/48 ,  C07H1/00 ,  C07H19/06 ,  C07H19/073 ,  C07H19/16 ,  C07H19/173 ,  C07H21/00 ,  C07H21/04 ,  C07K14/00 ,  C12N15/00 ,  C12N15/09 ,  C12P19/34 ,  C12Q1/68 ,  G01N33/53 ,  G01N33/566

CPC分类号： A61K47/48084 ,  A61K47/48061 ,  A61K47/48176 ,  A61K47/48192 ,  C07H21/00 ,  C07K14/003 ,  C12Q1/6813 ,  C12Q1/682 ,  C12Q1/6839 ,  B01J2219/0072 ,  Y02P20/55

摘要： A composition of polymer molecules effective to bind, with substantially uniform binding affinity, to a single-stranded polynucleotide containing a target sequence of bases. The polymer molecules are composed of a sequence of base-pairing moieties effective to hydrogen bond to corresponding, complementary bases in the target sequence, under selected binding conditions, and a predominantly uncharged, achiral backbone supporting the base-pairing moieties at positions and in orientations which allow hydrogen bonding between the pairing moieties of the polymer and the corresponding complementary bases in the target sequence. The composition has diagnostic uses, in a solid-support assay system, and therapeutic uses involving inhibition or inactivation of target polynucleotides.

摘要翻译： 具有以基本上均匀的结合亲和力结合含有靶基序列的单链多核苷酸的聚合物分子的组合物。 聚合物分子由选择的结合条件下有效氢键键合到靶序列中相应的互补碱基的碱基配对部分的序列和在位置和取向上支持碱基配对部分的主要不带电的非手性主链组成 其允许聚合物的配对部分与目标序列中相应的互补碱基之间的氢键合。 组合物具有诊断用途，在固体支持测定系统中，以及涉及靶多核苷酸的抑制或失活的治疗用途。