公开(公告)号：US07252991B2

公开(公告)日：2007-08-07

申请号：US10260516

申请日：2002-10-01

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/867 ,  C12N15/48 ,  C12N15/63 ,  C12N15/64 ,  A61K48/00

CPC分类号： C12N7/00 ,  A61K48/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C07K2319/00 ,  C12N15/86 ,  C12N15/87 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

摘要翻译： 本发明提供了一种新型的逆转录病毒包装系统，其中在哺乳动物细胞中稳定或瞬时转染之后，由高表达质粒产生逆转录病毒包装质粒和可包装载体转录物。 在这些转染的哺乳动物细胞中产生高滴度的重组逆转录病毒，然后可以通过共培养或上清液感染来转导哺乳动物靶细胞。 本发明的方法包括使用新型逆转录病毒包装质粒和载体以高效率通过共培养或上清液感染将原代人细胞（包括T细胞和人造血干细胞）转染外来基因。 本发明可用于快速生产高滴度病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。

公开(公告)号：US5858740A

公开(公告)日：1999-01-12

申请号：US438582

申请日：1995-05-10

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/76 ,  A61K48/00 ,  C07H21/04 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/92 ,  C12N15/10 ,  C12N15/86

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/86 ,  C12N15/87 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

摘要翻译： 本发明提供了一种新的逆转录病毒包装系统，其中通过在人细胞中转染从高表达质粒产生逆转录病毒包装构建体和可包装载体转录物。 在感染的细胞中产生高滴度的重组逆转录病毒。 本发明的方法包括使用新型逆转录病毒构建体通过以高效率的共培养将外源基因转导到包含T细胞和人造血干细胞的原代人细胞中。 本发明可用于快速生产高病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。

公开(公告)号：US5686279A

公开(公告)日：1997-11-11

申请号：US258152

申请日：1994-06-10

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/76 ,  A61K48/00 ,  C07H21/04 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/92 ,  C12N15/64 ,  C12N15/86

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/86 ,  C12N15/87 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

摘要翻译： 本发明提供了一种新的逆转录病毒包装系统，其中通过在人细胞中转染从高表达质粒产生逆转录病毒包装构建体和可包装载体转录物。 在感染的细胞中产生高滴度的重组逆转录病毒。 本发明的方法包括使用新型逆转录病毒构建体通过以高效率的共培养将外源基因转导到包含T细胞和人造血干细胞的原代人细胞中。 本发明可用于快速生产高病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。

公开(公告)号：US6051427A

公开(公告)日：2000-04-18

申请号：US517488

申请日：1995-08-21

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas L. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas L. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K48/00 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/00 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/91 ,  C12N15/86

CPC分类号： C12N15/86 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/87 ,  C12N7/00 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13023 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging plasmids and packagable vector transcripts are produced from high expression plasmids after stable or transient transfection in mammalian cells. High titers of recombinant retrovirus are produced in these transfected mammalian cells and can then transduce a mammalian target cell by cocultivation or supernatant infection. The methods of the invention include the use of the novel retroviral packaging plasmids and vectors to transduce primary human cells, including T cells and human hematopoietic stem cells, with foreign genes by cocultivation or supernatant infection at high efficiencies. The invention is useful for the rapid production of high titer viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

公开(公告)号：US5834256A

公开(公告)日：1998-11-10

申请号：US76299

申请日：1993-06-11

申请人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

发明人： Mitchell H. Finer ,  Margo R. Roberts ,  Thomas J. Dull ,  Krisztina M. Zsebo ,  Lu Qin ,  Deborah A. Farson

IPC分类号： C12N15/09 ,  A61K31/70 ,  A61K35/76 ,  A61K48/00 ,  C07H21/04 ,  C07K14/15 ,  C07K14/72 ,  C07K14/725 ,  C07K14/73 ,  C07K16/10 ,  C12N5/10 ,  C12N7/00 ,  C12N7/04 ,  C12N15/867 ,  C12N15/87 ,  C12R1/92 ,  C12P19/34 ,  C12N15/63 ,  C12N15/86

CPC分类号： C12N7/00 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70514 ,  C07K14/721 ,  C07K16/10 ,  C12N15/86 ,  C12N15/87 ,  A61K48/00 ,  C07K2319/00 ,  C12N2740/13022 ,  C12N2740/13043 ,  C12N2740/13052 ,  C12N2740/13062

摘要： The invention provides a novel retroviral packaging system, in which retroviral packaging constructs and packagable vector transcripts are produced from high expression plasmids by transfection in human cells. High titers of recombinant retrovirus are produced in infected cells. The methods of the invention include the use of the novel retroviral constructs to transduce primary human cells, including T cells and bone marrow stem cells, with foreign genes by cocultivation at high efficiencies. The invention is useful for the rapid production of high viral supernatants, and to transduce with high efficiency cells that are refractory to transduction by conventional means.

摘要翻译： 本发明提供了一种新的逆转录病毒包装系统，其中通过在人细胞中转染从高表达质粒产生逆转录病毒包装构建体和可包装载体转录物。 在感染的细胞中产生高滴度的重组逆转录病毒。 本发明的方法包括使用新的逆转录病毒构建体通过以高效率的共培养将外源基因转导到包括T细胞和骨髓干细胞的原代人细胞。 本发明可用于快速生产高病毒上清液，并且可以通过常规方法转导难以转导的高效细胞。