利索-全球专利检索

  1. 登录
  2. 注册

搜索结果

5 条记录 (耗时 0.012 秒)

    Enzyme-labeled immunoassay and device therefor
    1.
    发明授权
    Enzyme-labeled immunoassay and device therefor 失效
    酶标记免疫测定及其装置

    公开(公告)号：US06221625B1

    公开(公告)日：2001-04-24

    申请号：US09017214

    申请日：1998-02-02

    申请人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

    发明人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

    IPC分类号： G01N3353

    CPC分类号： G01N33/581 ,  G01N33/542 ,  Y10S435/97 ,  Y10S436/81

    摘要： An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor. A device for performing this enzyme-labeled immunoassay includes an absorbent material capable of transporting a developing solution by capillary action, the absorbent material including (a) a developing liquid application zone, (b) an enzyme-labeled reagent zone containing an enzyme-labeled reagent, (c) a sample receiving zone, and (d) an indicator reagent zone capable of immobilising the enzyme-labeled reagent after the reaction of the test sample with the enzyme-labeled reagent in an amount dependent on the assay result, with an enzyme inhibitor being applied to a portion in the absorbent material upstream of the enzyme-labeled reagent zone, which enzyme inhibitor prevents the formation of a signal from a predetermined time on after the enzyme-labeled reagent is immobilised at the indicator reagent zone.

    摘要翻译： 通过以下步骤进行酶标记的免疫测定：使测试样品与酶标记的试剂反应，允许底物与酶反应以形成信号，并固定酶标记的试剂，同时防止 使用酶抑制剂从固定酶标记的试剂之后的预定时间进一步形成信号。 用于进行该酶标记免疫测定的装置包括能够通过毛细作用输送显影液的吸收材料，所述吸收材料包括（a）显影液体施用区，（b）酶标记的试剂区，其含有酶标记的 试剂，（c）样品接收区，和（d）指示剂试剂区，其能够在测试样品与酶标记的试剂反应后以取决于测定结果的量固定酶标记的试剂， 酶抑制剂被施加到酶标记试剂区上游的吸收材料的一部分，该酶抑制剂防止在酶标记的试剂固定在指示剂试剂区之后的预定时间内形成信号。

    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method
    3.
    发明授权
    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method 有权
    非特异性杂交抑制剂，临床检查试剂和临床检查方法

    公开(公告)号：US08252531B2

    公开(公告)日：2012-08-28

    申请号：US10476069

    申请日：2002-04-25

    申请人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    发明人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832

    摘要： The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.

    摘要翻译： 本发明涉及能够提高杂交反应精度，抑制非特异性杂交的抑制剂，临床诊断试剂和临床分析方法，临床分析中的非特异性杂交被抑制，容易检测到靶核酸 准确度高。 抑制剂和临床诊断试剂含有具有非特异性杂交抑制作用的聚合物（H）。 聚合物在其分子中具有羧基和砜基中的至少一个和磷酸胆碱样基团，并且具有1000至5000000的重均分子量。临床分析方法包括使样品与能够与 在抑制剂存在下的特定核酸物质。