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    Enzyme-labeled immunoassay and device therefor
    1.
    发明授权
    Enzyme-labeled immunoassay and device therefor 失效
    酶标记免疫测定及其装置

    公开(公告)号：US06221625B1

    公开(公告)日：2001-04-24

    申请号：US09017214

    申请日：1998-02-02

    申请人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

    发明人： Yoshihiro Ashihara ,  Mitsuo Isomura ,  Atsuka Sato

    IPC分类号： G01N3353

    CPC分类号： G01N33/581 ,  G01N33/542 ,  Y10S435/97 ,  Y10S436/81

    摘要： An enzyme-labeled immunoassay is performed by the steps of allowing a test sample to react with an enzyme-labeled reagent, allowing a substrate to react with the enzyme to form a signal, and immobilising the enzyme-labeled reagent, with the prevention of a further signal formation from a predetermined time on after the immobilisation of the enzyme-labeled reagent, using an enzyme inhibitor. A device for performing this enzyme-labeled immunoassay includes an absorbent material capable of transporting a developing solution by capillary action, the absorbent material including (a) a developing liquid application zone, (b) an enzyme-labeled reagent zone containing an enzyme-labeled reagent, (c) a sample receiving zone, and (d) an indicator reagent zone capable of immobilising the enzyme-labeled reagent after the reaction of the test sample with the enzyme-labeled reagent in an amount dependent on the assay result, with an enzyme inhibitor being applied to a portion in the absorbent material upstream of the enzyme-labeled reagent zone, which enzyme inhibitor prevents the formation of a signal from a predetermined time on after the enzyme-labeled reagent is immobilised at the indicator reagent zone.

    摘要翻译： 通过以下步骤进行酶标记的免疫测定：使测试样品与酶标记的试剂反应，允许底物与酶反应以形成信号，并固定酶标记的试剂，同时防止 使用酶抑制剂从固定酶标记的试剂之后的预定时间进一步形成信号。 用于进行该酶标记免疫测定的装置包括能够通过毛细作用输送显影液的吸收材料，所述吸收材料包括（a）显影液体施用区，（b）酶标记的试剂区，其含有酶标记的 试剂，（c）样品接收区，和（d）指示剂试剂区，其能够在测试样品与酶标记的试剂反应后以取决于测定结果的量固定酶标记的试剂， 酶抑制剂被施加到酶标记试剂区上游的吸收材料的一部分，该酶抑制剂防止在酶标记的试剂固定在指示剂试剂区之后的预定时间内形成信号。

    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method
    3.
    发明授权
    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method 有权
    非特异性杂交抑制剂，临床检查试剂和临床检查方法

    公开(公告)号：US08252531B2

    公开(公告)日：2012-08-28

    申请号：US10476069

    申请日：2002-04-25

    申请人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    发明人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832

    摘要： The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.

    摘要翻译： 本发明涉及能够提高杂交反应精度，抑制非特异性杂交的抑制剂，临床诊断试剂和临床分析方法，临床分析中的非特异性杂交被抑制，容易检测到靶核酸 准确度高。 抑制剂和临床诊断试剂含有具有非特异性杂交抑制作用的聚合物（H）。 聚合物在其分子中具有羧基和砜基中的至少一个和磷酸胆碱样基团，并且具有1000至5000000的重均分子量。临床分析方法包括使样品与能够与 在抑制剂存在下的特定核酸物质。

    Immunoassay element and process for immunoassay
    6.
    发明授权
    Immunoassay element and process for immunoassay 失效
    免疫测定元件和免疫测定方法

    公开(公告)号：US06589754B1

    公开(公告)日：2003-07-08

    申请号：US08946685

    申请日：1997-10-08

    申请人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Hiroshi Shinoki ,  Yoshihiro Ashihara ,  Naofumi Hora ,  Masashi Ogawa

    发明人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Hiroshi Shinoki ,  Yoshihiro Ashihara ,  Naofumi Hora ,  Masashi Ogawa

    IPC分类号： G01N3353

    CPC分类号： G01N33/54386

    摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.

    摘要翻译： 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。

    Chemiluminescence enhancer
    8.
    发明授权
    Chemiluminescence enhancer 有权
    化学发光增强剂

    公开(公告)号：US07276343B2

    公开(公告)日：2007-10-02

    申请号：US10518586

    申请日：2003-06-24

    申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/553 ,  G01N33/566 ,  G01N21/75 ,  G01N21/76 ,  C12Q1/00 ,  C12N9/00 ,  C09K3/00

    CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

    摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理，以及化学发光 方法和使用化学发光增强剂的试剂盒。

    Liquid supply device and analytical test device
    9.
    发明授权
    Liquid supply device and analytical test device 失效
    液体供应装置和分析试验装置

    公开(公告)号：US6017494A

    公开(公告)日：2000-01-25

    申请号：US65725

    申请日：1998-04-24

    申请人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Yuko Fujiwara

    发明人： Yoshihiro Ashihara ,  Mitsushi Gotanda ,  Akira Hasegawa ,  Yuko Fujiwara

    IPC分类号： G01N31/22 ,  B01L3/00 ,  G01N1/10 ,  G01N33/543 ,  G01N33/558 ,  G01N35/02 ,  G01N21/29

    CPC分类号： G01N33/558 ,  B01L3/5023 ,  B01L2300/0672 ,  B01L2300/0825 ,  B01L2400/0406 ,  B01L2400/0633 ,  B01L2400/0683

    摘要： A liquid supply device for transporting a liquid to a predetermined portion includes a liquid container for holding a liquid therein, which has an opening, a sealing sheet member which is impermeable but rupturable and seals the opening of the container, and an absorbent material which is capable of transporting the liquid by capillary action, the absorbent material being disposed on the sealing sheet member, out of contact with the liquid when the sealing sheet member is not ruptured, while when the sealing sheet member is ruptured and the absorbent material is brought into contact with the liquid, part of the absorbent material being continuously held within the liquid and transporting the liquid to the predetermined position. An analytical test device using the above liquid supply device is provided, which may be used, for instance, immunoassay.

    摘要翻译： 用于将液体输送到预定部分的液体供应装置包括：具有开口的用于保持液体的液体容器，具有不可渗透但可破裂并且密封容器的开口的密封片部件;以及吸收材料， 能够通过毛细作用输送液体，当密封片构件未破裂时，吸收材料设置在密封片构件上，与液体不接触，而当密封片构件破裂并且吸收材料被引入时 与液体接触，吸收材料的一部分连续地保持在液体内并将液体输送到预定位置。 提供了使用上述液体供给装置的分析测试装置，其可以用于例如免疫测定。