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1.发明授权公开(公告)号：US09040661B2
公开(公告)日：2015-05-26
申请号：US13996978
申请日：2011-12-20
Applicant: Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani , Shun-Cheng Li , Xing Li , Courtney Voss
Inventor： Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani , Shun-Cheng Li , Xing Li , Courtney Voss
CPC classification number: C07K1/22 , B01D15/3804 , B01J20/28004 , B01J20/28057 , B01J20/28069 , B01J20/28078 , B01J20/288 , B01J20/321 , B01J20/3217 , B01J20/3274 , B01J20/3278 , B01J20/3293 , C07K16/065 , C07K17/08
Abstract: Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2 (1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.
Abstract translation: 提供了具有优异的耐碱性的亲和层析的支持物和分离免疫球蛋白的方法。 含有由下式（1）表示的固定化蛋白质配体的亲和层析的载体：R-R2（1）其中R表示由4至30个氨基酸残基组成的多肽，其含有由ATK或ASK表示的氨基酸序列 ; 并且R 2表示由50-500个氨基酸残基组成的多肽，其含有由SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列组成的免疫球蛋白结合结构域，其部分序列或具有 这些序列具有70％以上的同一性; 条件是R 2与R结合的末端是免疫球蛋白结合结构域的C末端或N末端。
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Patent Agency Ranking
2.发明授权Alkali-resistant variants of protein A and their use in affinity chromatography 有权蛋白A的抗碱性变体及其在亲和层析中的应用公开(公告)号：US09051375B2
公开(公告)日：2015-06-09
申请号：US13997015
申请日：2011-12-21
Applicant: Shun-Cheng Li , Xing Li , Courtney Voss , Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani
Inventor： Shun-Cheng Li , Xing Li , Courtney Voss , Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani
CPC classification number: C07K14/31 , B01D15/3809 , B01J20/28057 , B01J20/28078 , B01J20/3204 , B01J20/3208 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K17/06
Abstract: The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.
Abstract translation: 本发明涉及具有耐碱特性的免疫球蛋白（Ig）结合蛋白。 在一个实施方案中，本发明提供Ig结合蛋白的变体，所述变体包含具有至少一个被组氨酸取代的天冬酰胺残基，丝氨酸，天冬氨酸或苏氨酸残基的Ig结合蛋白。 当与野生型Ig结合相比时，至少一种取代可赋予变体Ig结合蛋白在碱性溶液中增加的稳定性。 本发明还涉及用于亲和分离包含本发明的Ig结合蛋白的免疫球蛋白的基质，以及使用本发明的Ig结合蛋白质从混合物组合物中分离免疫球蛋白的方法。
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公开(公告)号：US11274129B2
公开(公告)日：2022-03-15
申请号：US16308729
申请日：2017-06-12
Applicant: Shun-Cheng Li
Inventor： Shun-Cheng Li , Xuguang Liu
IPC: C07K14/435 , G01N33/543 , G01N33/574
Abstract: There is provided method of profiling protein tyrosine phosphorylation of a sample, the method comprising: contacting the sample with an SH2 Superbinder in order to bind pTyr-including peptides contained in the sample with the SH2 Superbinder; isolating the bound pTyr-including peptides from the sample; and identifying the isolated pTyr-including peptides.
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公开(公告)号：US20200031885A1
公开(公告)日：2020-01-30
申请号：US16308729
申请日：2017-06-12
Applicant: Shun-Cheng LI
Inventor： Shun-Cheng LI , Xuguang LIU
IPC: C07K14/435 , G01N33/574 , G01N33/543
Abstract: There is provided method of profiling protein tyrosine phosphorylation of a sample, the method comprising: contacting the sample with an SH2 Superbinder in order to bind pTyr-including peptides contained in the sample with the SH2 Superbinder; isolating the bound pTyr-including peptides from the sample; and identifying the isolated pTyr-including peptides.
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5.发明申请NOVEL ALKALI-RESISTANT VARIANTS OF PROTEIN A AND THEIR USE IN AFFINITY CHROMATOGRAPHY 有权蛋白A的新型抗碱性变体及其在亲和色谱中的应用公开(公告)号：US20140031522A1
公开(公告)日：2014-01-30
申请号：US13997015
申请日：2011-12-21
Applicant: Shun-Cheng LI , Xing Li , Courtney Voss , Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani
Inventor： Shun-Cheng LI , Xing Li , Courtney Voss , Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani
IPC: C07K14/31
CPC classification number: C07K14/31 , B01D15/3809 , B01J20/28057 , B01J20/28078 , B01J20/3204 , B01J20/3208 , B01J20/3219 , B01J20/3274 , C07K1/22 , C07K17/06
Abstract: The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.
Abstract translation: 本发明涉及具有耐碱特性的免疫球蛋白（Ig）结合蛋白。 在一个实施方案中，本发明提供Ig结合蛋白的变体，所述变体包含具有至少一个被组氨酸取代的天冬酰胺残基，丝氨酸，天冬氨酸或苏氨酸残基的Ig结合蛋白。 当与野生型Ig结合相比时，至少一种取代可赋予变体Ig结合蛋白在碱性溶液中增加的稳定性。 本发明还涉及用于亲和分离包含本发明的Ig结合蛋白的免疫球蛋白的基质，以及使用本发明的Ig结合蛋白质从混合物组合物中分离免疫球蛋白的方法。
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6.发明申请公开(公告)号：US20140005357A1
公开(公告)日：2014-01-02
申请号：US13996978
申请日：2011-12-20
Applicant: Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani , Shun-Cheng Li , Xing Li , Courtney Voss
Inventor： Satoshi Nakamura , Tetsuo Fukuta , Yusuke Okano , Tomonori Shiotani , Kouji Tamori , Yu Otani , Shun-Cheng Li , Xing Li , Courtney Voss
IPC: C07K1/22
CPC classification number: C07K1/22 , B01D15/3804 , B01J20/28004 , B01J20/28057 , B01J20/28069 , B01J20/28078 , B01J20/288 , B01J20/321 , B01J20/3217 , B01J20/3274 , B01J20/3278 , B01J20/3293 , C07K16/065 , C07K17/08
Abstract: Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2 (1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.
Abstract translation: 提供了具有优异的耐碱性的亲和层析的支持物和分离免疫球蛋白的方法。 含有由下式（1）表示的固定化蛋白质配体的亲和层析的载体：R-R2（1）其中R表示由4至30个氨基酸残基组成的多肽，其含有由ATK或ASK表示的氨基酸序列 ; 并且R 2表示由50-500个氨基酸残基组成的多肽，其含有由SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列组成的免疫球蛋白结合结构域，其部分序列或具有 这些序列具有70％以上的同一性; 条件是R 2与R结合的末端是免疫球蛋白结合结构域的C末端或N末端。
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