公开(公告)号：US09051355B2

公开(公告)日：2015-06-09

申请号：US13636410

申请日：2011-03-23

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

IPC分类号： A61K38/00 ,  C07K1/22 ,  B01D15/38 ,  B01J20/286 ,  B01J20/288 ,  B01J20/32

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/286 ,  B01J20/288 ,  B01J20/3219 ,  B01J20/3274 ,  C07K2317/10

摘要： Provided are a filler for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. The filler for affinity chromatography is a filler in which a protein represented by the following formula (1) is immobilized on a carrier. R—R2  (1) wherein R represents an amino acid sequence consisting of 4 to 300 amino acid residues containing a region consisting of 4 to 20 contiguous histidine residues; and R2 represents an amino acid sequence capable of binding to immunoglobulin, the amino acid sequence consisting of 50 to 500 amino acid residues containing Z domain of Protein A or a fragment thereof, or a variant thereof, provided that the R binds to C-terminus or N-terminus of the R2.

摘要翻译： 提供了具有优异的耐碱性的亲和色谱法的填充剂和分离免疫球蛋白的方法。 用于亲和层析的填料是其中由下式（1）表示的蛋白质固定在载体上的填料。 R-R2（1）其中R表示由4至300个氨基酸残基组成的氨基酸序列，其含有由4至20个连续组氨酸残基组成的区域; 并且R 2表示能够结合免疫球蛋白的氨基酸序列，所述氨基酸序列由含有蛋白A的Z结构域或其片段的50至500个氨基酸残基或其变体组成，条件是R与C-末端结合 或R2的N-末端。

公开(公告)号：US20140031522A1

公开(公告)日：2014-01-30

申请号：US13997015

申请日：2011-12-21

申请人： Shun-Cheng LI ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

发明人： Shun-Cheng LI ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

IPC分类号： C07K14/31

CPC分类号： C07K14/31 ,  B01D15/3809 ,  B01J20/28057 ,  B01J20/28078 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K17/06

摘要： The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.

摘要翻译： 本发明涉及具有耐碱特性的免疫球蛋白（Ig）结合蛋白。 在一个实施方案中，本发明提供Ig结合蛋白的变体，所述变体包含具有至少一个被组氨酸取代的天冬酰胺残基，丝氨酸，天冬氨酸或苏氨酸残基的Ig结合蛋白。 当与野生型Ig结合相比时，至少一种取代可赋予变体Ig结合蛋白在碱性溶液中增加的稳定性。 本发明还涉及用于亲和分离包含本发明的Ig结合蛋白的免疫球蛋白的基质，以及使用本发明的Ig结合蛋白质从混合物组合物中分离免疫球蛋白的方法。

公开(公告)号：US20140005357A1

公开(公告)日：2014-01-02

申请号：US13996978

申请日：2011-12-20

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani ,  Shun-Cheng Li ,  Xing Li ,  Courtney Voss

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani ,  Shun-Cheng Li ,  Xing Li ,  Courtney Voss

IPC分类号： C07K1/22

CPC分类号： C07K1/22 ,  B01D15/3804 ,  B01J20/28004 ,  B01J20/28057 ,  B01J20/28069 ,  B01J20/28078 ,  B01J20/288 ,  B01J20/321 ,  B01J20/3217 ,  B01J20/3274 ,  B01J20/3278 ,  B01J20/3293 ,  C07K16/065 ,  C07K17/08

摘要： Provided are a support for affinity chromatography which has excellent alkali resistance, and a method for isolating immunoglobulin. A support for affinity chromatography, containing an immobilized protein ligand represented by the following formula (1): R—R2  (1) wherein R represents a polypeptide consisting of 4 to 30 amino acid residues that contains an amino acid sequence represented by ATK or ASK; and R2 represents a polypeptide consisting of 50 to 500 amino acid residues containing an immunoglobulin-binding domain consisting of an amino acid sequence represented by SEQ ID NO: 1 or SEQ ID NO: 2, the partial sequence thereof, or an amino acid sequence having 70% or more identity to these sequences; with the proviso that a terminus at which R2 binds to R is C-terminus or N-terminus of the immunoglobulin-binding domain.

摘要翻译： 提供了具有优异的耐碱性的亲和层析的支持物和分离免疫球蛋白的方法。 含有由下式（1）表示的固定化蛋白质配体的亲和层析的载体：R-R2（1）其中R表示由4至30个氨基酸残基组成的多肽，其含有由ATK或ASK表示的氨基酸序列 ; 并且R 2表示由50-500个氨基酸残基组成的多肽，其含有由SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列组成的免疫球蛋白结合结构域，其部分序列或具有 这些序列具有70％以上的同一性; 条件是R 2与R结合的末端是免疫球蛋白结合结构域的C末端或N末端。

公开(公告)号：US20130316892A1

公开(公告)日：2013-11-28

申请号：US13994495

申请日：2011-12-14

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

IPC分类号： B01J20/281

CPC分类号： B01J20/281 ,  B01J20/267 ,  B01J20/285 ,  C08B1/003 ,  C08B15/10 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08J3/16 ,  C08J3/24 ,  C08J2301/02 ,  C08K5/0025 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  Y02P20/542

摘要： Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

摘要翻译： 提供当用作色谱填料时可以以高流速使用的聚合物颗粒，即具有适合于大量加工的优异的电阻流速，并且还具有对蛋白质等靶分子的高结合力 粒子中含有适当的配体，以及聚合物粒子的制造方法。 特别是交联聚合物颗粒和交联聚合物颗粒的制备方法，多糖复合颗粒和多糖复合颗粒的制备方法，使用该聚合物颗粒的色谱用填料和用于抗体纯化的吸附剂。 本发明涉及A.一种多糖复合粒子的制造方法，其特征在于，包括以下工序（1）〜（3）：（1）制备多糖溶液的工序，其中将2种以上的多糖溶解于 离子液体 （2）将多糖溶液的液滴分散在与离子液体的相容性低的有机溶剂中的多糖溶液的液滴分散液的制备工序; 和（3）将多糖的复合体凝结而得到多糖复合粒子的凝固工序; 和B.一种制备交联聚合物颗粒的方法，该方法包括使溶解在离子液体中的聚合物与交联剂反应的步骤，同时聚合物在与 离子液体。

公开(公告)号：US07732051B2

公开(公告)日：2010-06-08

申请号：US11772616

申请日：2007-07-02

申请人： Kouji Tamori ,  Eiji Takamoto ,  Masaru Ueno ,  Tetsuo Fukuta ,  Tomohiro Uetsuhara ,  Mitsuhiro Murata

发明人： Kouji Tamori ,  Eiji Takamoto ,  Masaru Ueno ,  Tetsuo Fukuta ,  Tomohiro Uetsuhara ,  Mitsuhiro Murata

IPC分类号： B32B5/16

CPC分类号： G01N33/5434 ,  G01N2446/20 ,  G01N2446/86 ,  Y10S525/902 ,  Y10T428/2998

摘要： A method for producing magnetic particles includes forming a hydrophobic first polymer layer on the surface of a mother particle containing superparamagnetic particles, forming a second polymer layer having glycidyl groups at least on its surface on the first polymer layer, and introducing a polar group containing one or more of at least one atom selected from the group consisting of an oxygen atom, a nitrogen atom, and a sulfur atom by chemically modifying the glycidyl groups.

摘要翻译： 制造磁性粒子的方法包括在含有超顺磁性粒子的母粒子的表面形成疏水性第一聚合物层，在第一聚合物层上至少在其表面上形成具有缩水甘油基的第二聚合物层，并引入含有一个 或更多的选自氧原子，氮原子和硫原子的至少一个原子，通过化学改性缩水甘油基。

公开(公告)号：US07713627B2

公开(公告)日：2010-05-11

申请号：US11688653

申请日：2007-03-20

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Satoshi Katayose ,  Masaru Ueno ,  Mitsuhiro Murata

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Satoshi Katayose ,  Masaru Ueno ,  Mitsuhiro Murata

IPC分类号： B32B5/16

CPC分类号： G01N33/5434 ,  G01N33/54393 ,  Y10S525/921 ,  Y10T428/2991 ,  Y10T428/2998

摘要： Magnetic particles comprising a group shown by the following formula (1): wherein R1 and R2 individually represent a hydroxyl group, a group shown by the following formula (2), or a group shown by the following formula (3), provided that R1 and R2 are not both hydrogen atoms, wherein R3 represents an a linear, branched, or cyclic alkylene group having 2 to 6 carbon atoms or an arylene group, and wherein R4 is a hydrogen atom or an alkyl group.

摘要翻译： 包含由下式（1）表示的基团的磁性颗粒：其中R1和R2各自表示羟基，下式（2）表示的基团或下式（3）所示的基团，条件是R1 并且R 2不是氢原子，其中R 3表示具有2至6个碳原子的直链，支链或环状亚烷基或亚芳基，并且其中R4是氢原子或烷基。

公开(公告)号：US09352298B2

公开(公告)日：2016-05-31

申请号：US13994495

申请日：2011-12-14

申请人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

发明人： Satoshi Nakamura ,  Tetsuo Fukuta ,  Kazuhiro Ikkyu ,  Yu Otani ,  Hiroshi Kawai

IPC分类号： B01J20/22 ,  B01J20/281 ,  B01J20/285 ,  C08J3/16 ,  C08J3/24 ,  C08B1/00 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  C08B15/10 ,  C08B37/00 ,  C08B37/02 ,  C08B37/08 ,  B01J20/26

CPC分类号： B01J20/281 ,  B01J20/267 ,  B01J20/285 ,  C08B1/003 ,  C08B15/10 ,  C08B37/0018 ,  C08B37/0021 ,  C08B37/003 ,  C08B37/0039 ,  C08J3/16 ,  C08J3/24 ,  C08J2301/02 ,  C08K5/0025 ,  C08L1/02 ,  C08L1/28 ,  C08L5/00 ,  C08L5/02 ,  C08L5/08 ,  C08L5/12 ,  Y02P20/542

摘要： Provided are polymer particles which can be used at a high flow rate when used as a filler for chromatography, that is, has excellent resistance flow rate appropriate for processing in large quantities, and also has a high binding capacity for target molecules such as proteins when an appropriate ligand is contained in the particles, and a method for producing the polymer particles; specifically, crosslinked polymer particles and a method for producing the crosslinked polymer particles, polysaccharide composite particles and a method for producing the polysaccharide composite particles, a filler for chromatography using the polymer particles, and an adsorbent for antibody purification. Disclosed are: A. a method for producing polysaccharide composite particles, the method including the following steps (1) to (3): (1) a step of preparing a polysaccharide solution, in which two or more kinds of polysaccharides are dissolved in an ionic liquid; (2) a step of preparing a droplet dispersion liquid of the polysaccharide solution, in which liquid droplets of the polysaccharide solution are dispersed in an organic solvent having low compatibility with the ionic liquid; and (3) a coagulation step in which a composite of the polysaccharides are coagulated to obtain the polysaccharide composite particles; and B. a method for producing a crosslinked polymer particle, the method including a step of allowing a polymer dissolved in an ionic liquid, to react with a crosslinking agent while the polymer is subjected to droplet dispersion in an organic solvent having low compatibility with the ionic liquid.

摘要翻译： 提供当用作色谱填料时可以以高流速使用的聚合物颗粒，即具有适合于大量加工的优异的电阻流速，并且还具有对蛋白质等靶分子的高结合力 粒子中含有适当的配体，以及聚合物粒子的制造方法。 特别是交联聚合物颗粒和交联聚合物颗粒的制备方法，多糖复合颗粒和多糖复合颗粒的制备方法，使用该聚合物颗粒的色谱用填料和用于抗体纯化的吸附剂。 本发明涉及A.一种多糖复合粒子的制造方法，其特征在于，包括以下工序（1）〜（3）：（1）制备多糖溶液的工序，其中将2种以上的多糖溶解于 离子液体 （2）将多糖溶液的液滴分散在与离子液体的相容性低的有机溶剂中的多糖溶液的液滴分散液的制备工序; 和（3）将多糖的复合体凝结而得到多糖复合粒子的凝固工序; 和B.一种制备交联聚合物颗粒的方法，该方法包括使溶解在离子液体中的聚合物与交联剂反应的步骤，同时聚合物在与 离子液体。

公开(公告)号：US09051375B2

公开(公告)日：2015-06-09

申请号：US13997015

申请日：2011-12-21

申请人： Shun-Cheng Li ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

发明人： Shun-Cheng Li ,  Xing Li ,  Courtney Voss ,  Satoshi Nakamura ,  Tetsuo Fukuta ,  Yusuke Okano ,  Tomonori Shiotani ,  Kouji Tamori ,  Yu Otani

IPC分类号： C07K14/31 ,  C07K17/06 ,  C07K1/22 ,  B01D15/38 ,  B01J20/32 ,  B01J20/28

CPC分类号： C07K14/31 ,  B01D15/3809 ,  B01J20/28057 ,  B01J20/28078 ,  B01J20/3204 ,  B01J20/3208 ,  B01J20/3219 ,  B01J20/3274 ,  C07K1/22 ,  C07K17/06

摘要： The present invention relates to immunoglobulin (Ig)-binding proteins with alkali-resistance properties. In one embodiment, the present invention provides for a variant of an Ig-binding protein, the variant comprising the Ig-binding protein having at least one asparagine residue substituted with a histidine, a serine, an aspartic acid or a threonine residue. The at least one substitution may confer to the variant Ig-binding protein an increased stability in alkaline solutions when compared to the wild-type Ig-binding. The present invention relates also to matrices for affinity separation of immunoglobulins comprising the Ig-binding proteins of the present invention, and to methods of using the Ig-binding proteins of the present invention to separate immunoglobulins from mixture compositions.

摘要翻译： 本发明涉及具有耐碱特性的免疫球蛋白（Ig）结合蛋白。 在一个实施方案中，本发明提供Ig结合蛋白的变体，所述变体包含具有至少一个被组氨酸取代的天冬酰胺残基，丝氨酸，天冬氨酸或苏氨酸残基的Ig结合蛋白。 当与野生型Ig结合相比时，至少一种取代可赋予变体Ig结合蛋白在碱性溶液中增加的稳定性。 本发明还涉及用于亲和分离包含本发明的Ig结合蛋白的免疫球蛋白的基质，以及使用本发明的Ig结合蛋白质从混合物组合物中分离免疫球蛋白的方法。

公开(公告)号：US08703289B2

公开(公告)日：2014-04-22

申请号：US11588388

申请日：2006-10-27

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Mitsuhiro Murata ,  Masaru Ueno ,  Satoshi Katayose ,  Eiji Takamoto ,  Kiyoshi Kasai

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Mitsuhiro Murata ,  Masaru Ueno ,  Satoshi Katayose ,  Eiji Takamoto ,  Kiyoshi Kasai

IPC分类号： B32B5/16 ,  A61K9/14

CPC分类号： G01N33/54326 ,  C08F8/36 ,  C08F8/42 ,  C08F8/44 ,  C08F220/26 ,  C08F220/32 ,  C08F222/1006 ,  C08F257/02 ,  C08F265/04 ,  C08F291/00 ,  C08F2810/20 ,  C08L25/04 ,  C08L33/066 ,  C08L51/003 ,  G01N33/5434 ,  H01F1/0054 ,  Y10T428/2991 ,  Y10T428/2998 ,  C08L2666/06 ,  C08L2666/02 ,  C08F8/12 ,  C08F2220/325 ,  C08F2220/1825 ,  C08F2222/1013 ,  C08F212/08 ,  C08F212/36 ,  C08F220/14 ,  C08F2222/1026 ,  C08F2220/281 ,  C08F2220/282

摘要： The organic polymer particles comprise a carboxyl group and 2,3-dihydroxypropyl group, and the magnetic particles for diagnostics comprise fine magnetic material particles and a polymer part containing a hydrophilic polymer part and a crosslinked polymer part, a dry coating film obtained from a water dispersion thereof having a contact angle with water of 5° to 60°. The process for producing the carboxyl group-containing particles comprises a step of producing an ester bond by reacting a hydroxyl group in organic polymer particles having the hydroxyl group with a carboxylic anhydride. The organic polymer particles comprise a hydroxyl group originating from a 2,3-dihydroxypropyl group and a polyoxyethylene group.

摘要翻译： 有机聚合物颗粒包含羧基和2,3-二羟基丙基，用于诊断的磁性颗粒包括细小的磁性材料颗粒和含有亲水性聚合物部分和交联聚合物部分的聚合物部分，从水中获得的干燥涂膜 其与水的接触角为5°至60°的分散体。 制备含羧基颗粒的方法包括通过使具有羟基的有机聚合物颗粒中的羟基与羧酸酐反应而产生酯键的步骤。 有机聚合物颗粒包含源自2,3-二羟基丙基和聚氧乙烯基团的羟基。

公开(公告)号：US20110262748A1

公开(公告)日：2011-10-27

申请号：US13121124

申请日：2009-09-24

申请人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yuusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

发明人： Kouji Tamori ,  Tetsuo Fukuta ,  Masaaki Miyaji ,  Yong Wang ,  Takayoshi Abe ,  Yuusuke Okano ,  Masaki Momiyama ,  Takahiro Kawai

IPC分类号： B01D15/38 ,  C08F24/00

CPC分类号： C07K1/22 ,  B01J20/286 ,  B01J20/321 ,  B01J20/3219 ,  B01J20/3274 ,  Y10T428/29

摘要： An affinity chromatography packing material includes porous mother particles that include a copolymer of a monomer mixture including a crosslinkable vinyl monomer and an epoxy group-containing vinyl monomer, a ligand being bound to the porous mother particles, and the porous mother particles including a ring-opening epoxy group produced by ring-opening of the epoxy group included in the porous mother particles.

摘要翻译： 亲和层析填充材料包括多孔母体颗粒，其包括包含可交联乙烯基单体和含环氧基的乙烯基单体的单体混合物的共聚物，与多孔母体颗粒结合的配体，以及多孔母体颗粒， 开环环氧基，其由包含在多孔母体颗粒中的环氧基开环制得。