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公开(公告)号：US10995356B2
公开(公告)日：2021-05-04
申请号：US15569579
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Jason W. Bjork , Evan D. Brutinel , Adam J. Stanenas
Abstract: The present disclosure provides a culture device for enumerating colonies of microorganisms. The device can comprise a base, a coversheet, and a nonporous spacer member disposed therebetween. The spacer member comprises an aperture that defines a growth compartment. Disposed in the growth compartment are a cold water-soluble gelling agent, a dry oxygen-scavenging reagent, a dry buffer system, and an effective amount of a dry carbon dioxide-generating reagent. The buffer system is selected such that, when the growth compartment is hydrated with a predetermined volume of deionized water, an aqueous mixture with a pH less than or equal to 6.35 is formed.
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公开(公告)号：US20140356888A1
公开(公告)日：2014-12-04
申请号：US14368525
申请日：2012-12-18
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Christine A. Binsfeld , Patrick A. Mach , Mara S. Celt , Adam J. Stanenas
IPC: C12Q1/34
CPC classification number: C12Q1/10 , C12Q1/04 , C12Q1/34 , C12Q2304/00 , C12Q2334/00 , C12Y302/01023 , Y02A50/451
Abstract: A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.
Abstract translation: 提供了一种检测沙门氏菌微生物的方法。 该方法包括使用选择性生长培养基，通过沙门氏菌微生物转化为第一可检测产物的第一指示剂系统和通过β-半乳糖苷酶活性转化为第二可检测产物的第二指示剂系统。 该方法还包括接种生长培养基并在高于40℃的温度下孵育接种的生长培养基。
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公开(公告)号：US10519481B2
公开(公告)日：2019-12-31
申请号：US15593598
申请日：2017-05-12
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Christine A. Binsfeld , Patrick A. Mach , Mara S. Celt , Adam J. Stanenas
Abstract: A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.
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公开(公告)号：US09677111B2
公开(公告)日：2017-06-13
申请号：US14368525
申请日：2012-12-18
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Chirstine A. Binsfeld , Patrick A. Mach , Mara S. Celt , Adam J. Stanenas
CPC classification number: C12Q1/10 , C12Q1/04 , C12Q1/34 , C12Q2304/00 , C12Q2334/00 , C12Y302/01023 , Y02A50/451
Abstract: A method of detecting a Salmonella microorganism is provided. The method includes the use of a selective growth medium, a first indicator system that is converted to a first detectable product by a Salmonella microorganism, and a second indicator system that is converted to a second detectable product by β-galactosidase enzyme activity. The method further comprises inoculating the growth medium and incubating the inoculated growth medium at a temperature higher than 40 degrees C.
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公开(公告)号：US20180100131A1
公开(公告)日：2018-04-12
申请号：US15566427
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Evan D. Brutinel , Mary B. Forester , Adam J. Stanenas , Jason W. Bjork
CPC classification number: C12M1/045 , C12M1/005 , C12M1/007 , C12M1/14 , C12M23/14 , C12M23/22 , C12M25/02 , C12M25/06 , C12M41/34 , C12M41/36 , C12N1/20 , C12Q1/04
Abstract: A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.
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Patent Agency Ranking
公开(公告)号：US09920350B2
公开(公告)日：2018-03-20
申请号：US14368711
申请日：2012-12-19
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Chirstine A. Binsfeld , Patrick A. Mach , Mara S. Celt , Adam J. Stanenas
CPC classification number: C12Q1/10 , C12Q1/045 , G01N2333/255 , Y02A50/451
Abstract: A composition is provided for detecting a Salmonella microorganism in sample. The composition comprises at least one first selective agent that inhibits the growth of Gram-positive microorganisms, a first differential indicator system comprising at least one first differential indicator compound that is converted to a first detectable product by a Salmonella microorganism, and a second differential indicator system comprising a second differential indicator compound that is converted by urease enzyme activity to a second detectable product. Optionally, the composition may comprise a third differential indicator system comprising a third differential indicator compound that is converted by a β-galactosidase enzyme activity to a third detectable product. Methods of using the composition to detect a Salmonella microorganism are also provided.
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7.发明申请公开(公告)号：US20170355943A1
公开(公告)日：2017-12-14
申请号：US15519944
申请日：2015-11-16
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Evan D. Brutinel , Alexi J. Young , Adam J. Stanenas
CPC classification number: C12M23/04 , A61L2/081 , A61L2/087 , C12M23/20 , C12M25/06 , C12M41/36 , C12M45/22 , C12Q1/04 , C12Q1/06
Abstract: Devices for the propagation or storage of microorganisms are provided including a first layer that has a first portion of a surface of the first layer, to which a first water-swellable gelling agent comprising a first clay is affixed. The devices further include a second layer that is separable from the first layer and has a first portion of a surface of the second layer, to which a second water-swellable gelling agent is affixed. Methods for detecting and enumerating at least one microorganism in a sample are provided. The methods include providing a device, separating the first layer from the second layer, adding an aliquot of a sample containing at least one microorganism onto the first or second water-swellable gelling agent to form an inoculated device, laminating the first layer back to the second layer, and incubating the inoculated device. Kits and methods of making the devices are also provided.
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公开(公告)号：US11702620B2
公开(公告)日：2023-07-18
申请号：US15566427
申请日：2016-04-26
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Evan D. Brutinel , Mary B. Forester , Adam J. Stanenas , Jason W. Bjork
CPC classification number: C12M1/045 , C12M1/005 , C12M1/007 , C12M1/14 , C12M23/14 , C12M23/22 , C12M25/02 , C12M25/06 , C12M41/34 , C12M41/36 , C12N1/20 , C12Q1/04
Abstract: A device for culturing anaerobic microorganisms is provided. The device comprises a body comprising a waterproof base, a waterproof coversheet attached to the base, and a growth compartment disposed between the base and the coversheet. The growth compartment has a perimeter and an opening that provides liquid access to the growth compartment. A portion of the perimeter is defined by a waterproof seal. The portion includes >50% of the perimeter. A dry cold water-soluble gelling agent is adhered to the base in the growth compartment. A dry first oxygen-scavenging reagent is disposed in the growth compartment.
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公开(公告)号：US20230041965A1
公开(公告)日：2023-02-09
申请号：US17758719
申请日：2021-02-08
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Neil Percy , Wensheng Xia , Adam J. Stanenas
Abstract: A culture device comprising an oxygen sensitive luminophore, typically an oxygen sensitive phosphor, such as a porphyrin, and methods of use for culturing and enumerating microorganisms
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公开(公告)号：US11072771B2
公开(公告)日：2021-07-27
申请号：US15763744
申请日：2016-09-27
Applicant: 3M INNOVATIVE PROPERTIES COMPANY
Inventor： Evan D. Brutinel , Jason W. Bjork , Adam J. Stanenas
Abstract: In one aspect, the present disclosure provides a device. The device includes a body comprising a waterproof base, a waterproof coversheet attached to the base, and a channel disposed between the base and the coversheet. The channel has a perimeter and an opening that provides liquid access to the channel. A portion of the perimeter is defined by a waterproof seal. A dry first oxygen-scavenging reagent and an indicator reagent for detecting sulfate reduction by a sulfate-reducing bacterium are disposed in the device between the base and the coversheet. The waterproof base comprises a plurality of open microcompartment structures facing the coversheet.
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