公开(公告)号：US20150276755A1

公开(公告)日：2015-10-01

申请号：US14438469

申请日：2013-10-25

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Chandra Shekhar Verma ,  David Philip Lane ,  Jia Wei Siau ,  Thomas Leonard Joseph ,  Adelene Yen Ling Sim

IPC: G01N33/68 ,  C12Q1/68

CPC classification number: G01N33/6845 ,  C12Q1/6883 ,  C12Q2600/156 ,  G01N2333/4748 ,  G01N2333/9015 ,  G01N2500/02

Abstract: The present disclosure provides a method of determining resistance of a biological molecule to inhibition of its interaction with a target molecule by an inhibitor of the biological molecule, the method comprising the steps of: a) co-compartmentalizing a gene encoding the biological molecule with the target molecule, or a gene encoding the biological molecule with a gene encoding the target molecule into an aqueous droplet disposed within a water-in-oil emulsion, and b) assaying for a complex comprising the biological molecule and the target molecule upon expression of the gene encoding the biological molecule and the gene encoding the target molecule, wherein detection of the complex in the presence of the inhibitor indicates that the biological molecule is resistant to inhibition of its interaction with the target molecule by the inhibitor. Also provided are mutated HDM2 ubiquitin ligase polypeptides exhibiting resistance to Nutlin inhibition of p53 binding.

Abstract translation: 本公开提供了一种确定生物分子对生物分子的抑制剂与靶分子的相互作用的抗性的方法，所述方法包括以下步骤：a）将编码生物分子的基因与 目标分子或编码具有编码靶分子的基因的生物分子的基因转化成布置在油包水乳液中的水滴，以及b）在表达后，测定包含生物分子和靶分子的复合物 编码生物分子的基因和编码靶分子的基因，其中在抑制剂存在下检测复合物表明生物分子对抑制剂与靶分子的相互作用的抑制具有抗性。 还提供了显示出对Nutlin抑制p53结合的抗性的突变的HDM2泛素连接酶多肽。

公开(公告)号：US09863937B2

公开(公告)日：2018-01-09

申请号：US14431723

申请日：2013-09-26

Applicant: Agency for Science, Technology and Research

Inventor： Sydney Brenner ,  Yin Nah Teo ,  Farid Ghadessy ,  Leng Peng Walter Goh ,  Min Yen Lee

IPC: C07C255/41 ,  G01N33/542 ,  C07D219/08 ,  C07D401/14 ,  C07D417/06 ,  C07D455/04 ,  C07D495/04 ,  C07D519/00 ,  G01N33/58

CPC classification number: G01N33/542 ,  C07C255/41 ,  C07C2603/50 ,  C07D219/08 ,  C07D401/14 ,  C07D417/06 ,  C07D455/04 ,  C07D495/04 ,  C07D519/00 ,  G01N33/582

Abstract: The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.

公开(公告)号：US11414681B2

公开(公告)日：2022-08-16

申请号：US16430410

申请日：2019-06-03

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Jia Wei Siau ,  Peter Droge ,  Harshyaa Makhija ,  Shree Harsh Vijaya Chandra ,  Sabrina Peter

IPC: C12N9/12 ,  C12N9/22 ,  C12N1/15 ,  C12N1/21 ,  C12P19/34 ,  C12N15/90

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

公开(公告)号：US10344301B2

公开(公告)日：2019-07-09

申请号：US15502783

申请日：2015-08-11

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Farid Ghadessy ,  Jia Wei Siau ,  Peter Droge ,  Harshyaa Makhija ,  Shree Harsh Vijaya Chandra ,  Sabrina Peter

IPC: C12N9/12 ,  C12N9/22 ,  C12N1/15 ,  C12N1/21 ,  C12P19/34 ,  C12N15/90

Abstract: The present invention refers to lambda integrases comprising at least one amino acid mutation at positions 43, 319 and 336 of the lambda integrase as set forth in SEQ ID NO: 1. The invention further refers to nucleic acid molecules comprising the nucleotide sequence encoding the mutant lambda integrase and to host cells containing these nucleic acid molecules. The invention also refers to methods of recombining a nucleic acid of interest into a target nucleic acid in the presence of the mutant lambda integrase and sequence specific recombination kits.

公开(公告)号：US20160195519A1

公开(公告)日：2016-07-07

申请号：US14431723

申请日：2013-09-26

Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH

Inventor： Sydney Brenner ,  Yin Nah Teo ,  Farid Ghadessy ,  Leng Peng Walter Goh ,  Min Yen Lee

IPC: G01N33/542 ,  C07D495/04 ,  G01N33/58 ,  C07C255/41 ,  C07D455/04 ,  C07D519/00 ,  C07D219/08 ,  C07D401/14

CPC classification number: G01N33/542 ,  C07C255/41 ,  C07C2603/50 ,  C07D219/08 ,  C07D401/14 ,  C07D417/06 ,  C07D455/04 ,  C07D495/04 ,  C07D519/00 ,  G01N33/582

Abstract: The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.

Abstract translation: 本发明涉及用于检测蛋白质和配体之间相互作用的方法和组合物，其包括：（i）将至少一种荧光分子转子与所述配体或蛋白质结合; 和（ii）检测所述荧光分子转子在所述结合的荧光分子转子与所述配体或蛋白质中的另一个接触之后发射的荧光的变化，从而检测所述配体和所述蛋白质之间的相互作用，其中所述荧光分子转子包括： 旋转的？ 给电子部分; 电子接受部分; 和π共轭接头。