-
公开(公告)号：US09863937B2
公开(公告)日：2018-01-09
申请号：US14431723
申请日：2013-09-26
Applicant: Agency for Science, Technology and Research
Inventor： Sydney Brenner , Yin Nah Teo , Farid Ghadessy , Leng Peng Walter Goh , Min Yen Lee
IPC: C07C255/41 , G01N33/542 , C07D219/08 , C07D401/14 , C07D417/06 , C07D455/04 , C07D495/04 , C07D519/00 , G01N33/58
CPC classification number: G01N33/542 , C07C255/41 , C07C2603/50 , C07D219/08 , C07D401/14 , C07D417/06 , C07D455/04 , C07D495/04 , C07D519/00 , G01N33/582
Abstract: The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.
-
公开(公告)号：US20160145280A1
公开(公告)日：2016-05-26
申请号：US14784939
申请日：2014-04-21
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Sydney Brenner , Yin Nah Teo , Pee Mei Tan
CPC classification number: C12Q1/6818 , C07D207/46 , C07D493/10 , C07F7/12 , C07F7/1804 , C07F9/1651 , C07F9/2408 , C07F9/65522 , C07F9/6561 , C07F9/65616 , C07H21/04 , C09B11/08 , C09B23/06 , C09B29/0003 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , G01N2201/068
Abstract: The invention relates to cleavable chemistry in general, and in particular, to tunable fluorescence using cleavable linkers present in fluorochrome-quencher conjugates.
Abstract translation: 本发明一般涉及可切割化学，特别涉及使用荧光染料 - 猝灭剂缀合物中存在的可切割接头的可调谐荧光。
-
公开(公告)号：US09845493B2
公开(公告)日：2017-12-19
申请号：US14784939
申请日：2014-04-21
Applicant: Agency for Science, Technology and Research
Inventor： Sydney Brenner , Yin Nah Teo , Pee Mei Tan
IPC: C12Q1/68 , C07H19/04 , C09B29/01 , C09B23/06 , C09B11/08 , C07H21/04 , C07D493/10 , C07D207/46 , C07F9/655 , C07F9/6561 , C07F9/165 , C07F9/24 , C07F7/12 , C07F7/18 , G01N21/64
CPC classification number: C12Q1/6818 , C07D207/46 , C07D493/10 , C07F7/12 , C07F7/1804 , C07F9/1651 , C07F9/2408 , C07F9/65522 , C07F9/6561 , C07F9/65616 , C07H21/04 , C09B11/08 , C09B23/06 , C09B29/0003 , G01N21/6428 , G01N2021/6432 , G01N2021/6439 , G01N2201/068
Abstract: The invention relates to cleavable chemistry in general, and in particular, to tunable fluorescence using cleavable linkers present in fluorochrome-quencher conjugates.
-
公开(公告)号：US20160195519A1
公开(公告)日：2016-07-07
申请号：US14431723
申请日：2013-09-26
Applicant: AGENCY FOR SCIENCE, TECHNOLOGY AND RESEARCH
Inventor： Sydney Brenner , Yin Nah Teo , Farid Ghadessy , Leng Peng Walter Goh , Min Yen Lee
IPC: G01N33/542 , C07D495/04 , G01N33/58 , C07C255/41 , C07D455/04 , C07D519/00 , C07D219/08 , C07D401/14
CPC classification number: G01N33/542 , C07C255/41 , C07C2603/50 , C07D219/08 , C07D401/14 , C07D417/06 , C07D455/04 , C07D495/04 , C07D519/00 , G01N33/582
Abstract: The present invention relates to methods and compositions for detecting an interaction between a protein and a ligand, comprising: (i) binding at least one fluorescent molecular rotor to said ligand or protein; and (ii) detecting a change in fluorescence emitted by said fluorescent molecular rotor after contact of the bound fluorescent molecular rotor with the other of said ligand or protein, thereby detecting an interaction between the ligand and the protein, wherein the fluorescent molecular rotor comprises: a rotating ?-bond; an electron-donating moiety; an electron-accepting moiety; and a ?-conjugated linker.
Abstract translation: 本发明涉及用于检测蛋白质和配体之间相互作用的方法和组合物，其包括：（i）将至少一种荧光分子转子与所述配体或蛋白质结合; 和（ii）检测所述荧光分子转子在所述结合的荧光分子转子与所述配体或蛋白质中的另一个接触之后发射的荧光的变化，从而检测所述配体和所述蛋白质之间的相互作用，其中所述荧光分子转子包括： 旋转的？ 给电子部分; 电子接受部分; 和π共轭接头。
-
-
-
Search Results
4
records
(took 0.019 seconds)
