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    LASER DESORPTION IONIZATION AND PEPTIDE SEQUENCING ON LASER INDUCED SILICON MICROCOLUMN ARRAYS
    1.
    发明申请
    LASER DESORPTION IONIZATION AND PEPTIDE SEQUENCING ON LASER INDUCED SILICON MICROCOLUMN ARRAYS 有权
    在激光诱导硅微阵列上的激光解吸离子和肽序列

    公开(公告)号：US20090321626A1

    公开(公告)日：2009-12-31

    申请号：US11674671

    申请日：2007-02-14

    申请人： Akos Vertes ,  Yong Chen

    发明人： Akos Vertes ,  Yong Chen

    IPC分类号： H01J49/26 ,  B32B3/10 ,  B29C59/16

    CPC分类号： B29C59/16 ,  B29C2059/023 ,  B29C2791/009 ,  H01J49/0418 ,  Y10T428/24479

    摘要： The present invention provides a method of producing a laser-patterned silicon surface, especially silicon wafers for use in laser desorption ionization (LDI-MS) (including MALDI-MS and SELDI-MS), devices containing the same, and methods of testing samples employing the same. The surface is prepared by subjecting a silicon substrate to multiple laser shots from a high-power picosecond or femtosecond laser while in a processing environment, e.g., underwater, and generates a remarkable homogenous microcolumn array capable of providing an improved substrate for LDI-MS.

    摘要翻译： 本发明提供了一种制造激光图案化硅表面的方法，特别是用于激光解吸电离（LDI-MS）（包括MALDI-MS和SELDI-MS）的硅晶片，含有该硅表面的器件，以及测试样品的方法 用同样的 该表面是通过在处理环境（例如水下）中使硅衬底经受来自高功率皮秒或飞秒激光器的多次激光照射，并且产生能够为LDI-MS提供改进的衬底的显着均匀的微柱阵列。

    Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays
    2.
    发明授权
    Laser desorption ionization and peptide sequencing on laser induced silicon microcolumn arrays 有权
    激光解吸电离和激光诱导硅微柱阵列上的肽测序

    公开(公告)号：US08084734B2

    公开(公告)日：2011-12-27

    申请号：US11674671

    申请日：2007-02-14

    申请人： Akos Vertes ,  Yong Chen

    发明人： Akos Vertes ,  Yong Chen

    IPC分类号： H01J49/04 ,  H01J49/16

    CPC分类号： B29C59/16 ,  B29C2059/023 ,  B29C2791/009 ,  H01J49/0418 ,  Y10T428/24479

    摘要： The present invention provides a method of producing a laser-patterned silicon surface, especially silicon wafers for use in laser desorption ionization (LDI-MS) (including MALDI-MS and SELDI-MS), devices containing the same, and methods of testing samples employing the same. The surface is prepared by subjecting a silicon substrate to multiple laser shots from a high-power picosecond or femtosecond laser while in a processing environment, e.g., underwater, and generates a remarkable homogenous microcolumn array capable of providing an improved substrate for LDI-MS.

    摘要翻译： 本发明提供了一种制造激光图案化硅表面的方法，特别是用于激光解吸电离（LDI-MS）（包括MALDI-MS和SELDI-MS）的硅晶片，含有该硅表面的器件，以及测试样品的方法 用同样的 该表面是通过在处理环境（例如水下）中使硅衬底经受来自高功率皮秒或飞秒激光器的多次激光照射，并且产生能够为LDI-MS提供改进的衬底的显着均匀的微柱阵列。

    Subcellular analysis by laser ablation electrospray ionization mass spectrometry
    3.
    发明授权
    Subcellular analysis by laser ablation electrospray ionization mass spectrometry 有权
    通过激光消融电喷雾电离质谱法进行亚细胞分析

    公开(公告)号：US08901487B2

    公开(公告)日：2014-12-02

    申请号：US13045277

    申请日：2011-03-10

    申请人： Akos Vertes ,  Jessica A. Stolee ,  Bindesh Shrestha

    发明人： Akos Vertes ,  Jessica A. Stolee ,  Bindesh Shrestha

    IPC分类号： H01J49/00 ,  H01J49/26 ,  H05H3/02

    CPC分类号： H01J49/26 ,  H05H3/02

    摘要： In various embodiments, a method of laser ablation electrospray ionization mass spectrometry (LAESI-MS) may generally comprise micro-dissecting a cell comprising at least one of a cell wall and a cell membrane to expose at least one subcellular component therein, ablating the at least one subcellular component by an infrared laser pulse to form an ablation plume, intercepting the ablation plume by an electrospray plume to form ions, and detecting the ions by mass spectrometry.

    摘要翻译： 在各种实施例中，激光消融电喷雾离子化质谱法（LAESI-MS）的方法通常可以包括微细解剖包含细胞壁和细胞膜中的至少一个的细胞，以在其中暴露至少一种亚细胞组分， 通过红外激光脉冲至少一个亚细胞分量以形成消融羽流，通过电喷雾羽流截取消融羽流以形成离子，并通过质谱法检测离子。

    Protein microscope
    4.
    发明授权
    Protein microscope 有权
    蛋白质显微镜

    公开(公告)号：US08286260B2

    公开(公告)日：2012-10-09

    申请号：US12783122

    申请日：2010-05-19

    申请人： Akos Vertes ,  Mark E. Reeves ,  Fatah Kashanchi

    发明人： Akos Vertes ,  Mark E. Reeves ,  Fatah Kashanchi

    IPC分类号： H01J49/40

    CPC分类号： G01Q30/02 ,  G01Q60/22 ,  H01J49/0004

    摘要： A system and method for analyzing and imaging a sample containing molecules of interest combines modified MALDI mass spectrometer and SNOM devices and techniques and includes: (A) an atmospheric pressure or near-atmospheric pressure ionization region; (B) a sample holder for holding the sample; (C) a laser for illuminating said sample; (D) a mass spectrometer having at least one evacuated chamber; (E) an atmospheric pressure interface for connecting said ionization region and said mass spectrometer; (F) a scanning near-field optical microscopy instrument; (G) a recording device for recording topography and mass spectrum measurements made during scanning of the sample with the near-field probe; (H) a plotting device for plotting said topography and mass spectrum measurements as separate x-y mappings; and (I) an imaging device for providing images of the x-y mappings.

    摘要翻译： 用于分析和成像含有感兴趣的分子的样品的系统和方法结合了改进的MALDI质谱仪和SNOM装置和技术，并且包括：（A）大气压或近大气压电离区域; （B）用于保持样品的样品架; （C）用于照亮所述样品的激光器; （D）具有至少一个真空室的质谱仪; （E）用于连接所述电离区和所述质谱仪的大气压界面; （F）扫描近场光学显微镜仪器; （G）用于记录在用近场探针扫描样品期间进行的地形和质谱测量的记录装置; （H）用于将所述地形和质谱测量绘制为单独的x-y映射的绘图装置; 和（I）用于提供x-y映射的图像的成像装置。

    Nanophotonic production, modulation and switching of ions by silicon microcolumn arrays
    5.
    发明授权
    Nanophotonic production, modulation and switching of ions by silicon microcolumn arrays 有权
    通过硅微柱阵列的纳米磷光生产，离子的调制和切换

    公开(公告)号：US08110796B2

    公开(公告)日：2012-02-07

    申请号：US12689829

    申请日：2010-01-19

    申请人： Akos Vertes ,  Bennett N. Walker

    发明人： Akos Vertes ,  Bennett N. Walker

    IPC分类号： H01J49/16

    CPC分类号： H01J49/164 ,  H01J49/0031 ,  H01J49/0418

    摘要： The production and use of silicon microcolumn arrays that harvest light from a laser pulse to produce ions are described. The systems of the present invention seem to behave like a quasi-periodic antenna array with ion yields that show profound dependence on the plane of laser light polarization and the angle of incidence. By providing photonic ion sources, this enables enhanced control of ion production on a micro/nano scale and direct integration with miniaturized analytical devices.

    摘要翻译： 描述了从激光脉冲收集光以产生离子的硅微柱阵列的生产和使用。 本发明的系统似乎像具有离子产生的准周期性天线阵列，其表现出对激光偏振平面和入射角的深刻依赖性。 通过提供光子离子源，这可以增强对微/纳米级离子产生的控制，并与微型分析装置直接集成。

    NANOPHOTONIC PRODUCTION, MODULATION AND SWITCHING OF IONS BY SILICON MICROCOLUMN ARRAYS
    6.
    发明申请
    NANOPHOTONIC PRODUCTION, MODULATION AND SWITCHING OF IONS BY SILICON MICROCOLUMN ARRAYS 有权
    硅微波阵列的纳米生产，离子的调制和切换

    公开(公告)号：US20100207021A1

    公开(公告)日：2010-08-19

    申请号：US12689829

    申请日：2010-01-19

    申请人： Akos Vertes ,  Bennett N. Walker

    发明人： Akos Vertes ,  Bennett N. Walker

    IPC分类号： H01J49/26 ,  H01J49/04

    CPC分类号： H01J49/164 ,  H01J49/0031 ,  H01J49/0418

    摘要： The production and use of silicon microcolumn arrays that harvest light from a laser pulse to produce ions are described. The systems of the present invention seem to behave like a quasi-periodic antenna array with ion yields that show profound dependence on the plane of laser light polarization and the angle of incidence. By providing photonic ion sources, this enables enhanced control of ion production on a micro/nano scale and direct integration with miniaturized analytical devices.

    摘要翻译： 描述了从激光脉冲收集光以产生离子的硅微柱阵列的生产和使用。 本发明的系统似乎像具有离子产生的准周期性天线阵列，其表现出对激光偏振平面和入射角的深刻依赖性。 通过提供光子离子源，这可以增强对微/纳米级离子产生的控制，并与微型分析装置直接集成。