公开(公告)号：US20210237080A1

公开(公告)日：2021-08-05

申请号：US17140861

申请日：2021-01-04

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Volker L.S. KURZ

IPC: B01L3/00 ,  G01N33/00

Abstract: Methods for screening plant cells, particularly plant protoplasts, for disease resistant traits, and kits for performing such methods are provided. The methods are performed in a microfluidic device that includes a flow region and at least one growth chamber suitable for culturing and screening a plant protoplast. The at least one surface of the growth chamber of the microfluidic chip can include a covalently linked coating material or a surface modifying ligand. The kit can comprise a microfluidic chip in combination with a reagent for detecting the viability of the plant protoplast and, optionally, a surface conditioning reagent or a surface modification reagent.

公开(公告)号：US20210292751A1

公开(公告)日：2021-09-23

申请号：US17226914

申请日：2021-04-09

Applicant: BERKELEY LIGHTS, INC.

Inventor： Jason M. McEWEN ,  Troy A. LIONBERGER ,  Eric K. SACKMANN ,  Volker L.S. KURZ ,  Kellen C. MOBILIA

IPC: C12N15/10 ,  B01L3/00

Abstract: Systems, methods, and kits therefor, enabling rapid protein evolution are described herein. A system useful in the methods described herein include a DNA synthesis component; a microfluidic system including a microfluidic device having a microfluidic channel and sequestration pens; and a computing component, which is configured to analyze assay results and, based upon the analysis, design improved DNA sequences for iterative protein evolution. The microfluidic system is configured to permit correlation of DNA sequence on a bead to its location within the microfluidic device, permit cell free protein expression of a DNA sequence captured to a bead, and to permit assay of the protein so produced.

公开(公告)号：US20220250071A1

公开(公告)日：2022-08-11

申请号：US17534350

申请日：2021-11-23

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  J. Tanner NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN ,  Anupam SINGHAL ,  I-Jong LIN

IPC: B01L3/00 ,  G01N21/64 ,  B03C5/02 ,  B03C5/00 ,  B01L9/00 ,  G06T7/00

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.

公开(公告)号：US20190240665A1

公开(公告)日：2019-08-08

申请号：US16160816

申请日：2018-10-15

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Matthew E. FOWLER ,  Phillip J. M. ELMS ,  Kevin D. LOUTHERBACK ,  Randall D. LOWE, JR. ,  Jian GONG ,  Tanner J. NEVILL ,  Gang F. WANG ,  Gregory G. LAVIEU ,  John A. TENNEY ,  Aathavan KARUNAKARAN

IPC: B01L3/00 ,  G06T7/00 ,  B01L9/00

CPC classification number: B01L3/502761 ,  B01L9/527 ,  B01L2300/0829 ,  B03C5/005 ,  B03C5/026 ,  B03C2201/26 ,  G01N21/05 ,  G01N21/6452 ,  G01N21/6458 ,  G01N2201/0675 ,  G06T7/0014

Abstract: Methods, systems and kits are described herein for detecting the results of an assay. In particular, the methods, systems and devices of the present disclosure rely on a difference between the diffusion rates of a reporter molecule and an analyte of interest in order to quantify an amount of analyte in a microfluidic device. The analyte may be a secreted product of a biological micro-object.