公开(公告)号：US20210292751A1

公开(公告)日：2021-09-23

申请号：US17226914

申请日：2021-04-09

Applicant: BERKELEY LIGHTS, INC.

Inventor： Jason M. McEWEN ,  Troy A. LIONBERGER ,  Eric K. SACKMANN ,  Volker L.S. KURZ ,  Kellen C. MOBILIA

IPC: C12N15/10 ,  B01L3/00

Abstract: Systems, methods, and kits therefor, enabling rapid protein evolution are described herein. A system useful in the methods described herein include a DNA synthesis component; a microfluidic system including a microfluidic device having a microfluidic channel and sequestration pens; and a computing component, which is configured to analyze assay results and, based upon the analysis, design improved DNA sequences for iterative protein evolution. The microfluidic system is configured to permit correlation of DNA sequence on a bead to its location within the microfluidic device, permit cell free protein expression of a DNA sequence captured to a bead, and to permit assay of the protein so produced.

公开(公告)号：US20230201827A1

公开(公告)日：2023-06-29

申请号：US18167638

申请日：2023-02-10

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

CPC classification number: B01L3/502715 ,  B01L2200/0673 ,  B01L2200/16 ,  B01L2300/0636 ,  B01L2300/087 ,  B01L2300/0877

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20220161255A1

公开(公告)日：2022-05-26

申请号：US17452725

申请日：2021-10-28

Applicant: Berkeley Lights, Inc.

Inventor： Volker L.S. KURZ ,  Jason M. MCEWEN ,  Kellen C. MOBILIA ,  Alexander J. MASTROIANNI ,  Joshua J. CARDIEL RIVERA

IPC: B01L3/00

Abstract: In biosciences and related fields, it can be useful to study cells in isolation so that cells having unique and desirable properties can be identified within a heterogenous mixture of cells. Processes and methods disclosed herein provide for encapsulating cells within a microfluidic device and assaying the encapsulated cells. Encapsulation can, among other benefits, facilitate analyses of cells that generate secretions of interest which would otherwise rapidly diffuse away or mix with the secretions of other cells.

公开(公告)号：US20230023831A1

公开(公告)日：2023-01-26

申请号：US17930649

申请日：2022-09-08

Applicant: BERKELEY LIGHTS, INC

Inventor： Kristin G. BEAUMONT ,  Peter J. BEEMILLER ,  Volker L.S. KURZ ,  Gregory G. LAVIEU ,  Xiaohua WANG ,  Aathavan KARUNAKARAN

IPC: G01N33/543 ,  B01L3/00 ,  G01N33/545 ,  G01N33/58

Abstract: In situ-generated microfluidic capture structures incorporating a solidified polymer network, methods of preparation and use, compositions and kits therefor are described. Microfluidic capture structures may be advantageously used for assays performed within the microfluidic environment, providing flexibility in assaying micro-objects such as biological cells. Assay reagents and analytes may be incorporated within the microfluidic capture structures.

公开(公告)号：US20210237080A1

公开(公告)日：2021-08-05

申请号：US17140861

申请日：2021-01-04

Applicant: Berkeley Lights, Inc.

Inventor： Troy A. LIONBERGER ,  Volker L.S. KURZ

IPC: B01L3/00 ,  G01N33/00

Abstract: Methods for screening plant cells, particularly plant protoplasts, for disease resistant traits, and kits for performing such methods are provided. The methods are performed in a microfluidic device that includes a flow region and at least one growth chamber suitable for culturing and screening a plant protoplast. The at least one surface of the growth chamber of the microfluidic chip can include a covalently linked coating material or a surface modifying ligand. The kit can comprise a microfluidic chip in combination with a reagent for detecting the viability of the plant protoplast and, optionally, a surface conditioning reagent or a surface modification reagent.