公开(公告)号：US11926871B2

公开(公告)日：2024-03-12

申请号：US17215683

申请日：2021-03-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Lucas Frenz ,  Jeremy Agresti

IPC: C12Q1/6874 ,  C12Q1/6806

CPC classification number: C12Q1/6874 ,  C12Q1/6806 ,  C12Q1/6806 ,  C12Q2565/514

Abstract: Provided herein are compositions and methods for generating phase-shift barcode oligonucleotides for library construction for next-generation sequencing. In some cases, barcode oligonucleotides are attached to particles or beads. Also provided are methods and kits for using the phase-shift barcode oligonucleotides in sequencing assays.

公开(公告)号：US11725206B2

公开(公告)日：2023-08-15

申请号：US17102111

申请日：2020-11-23

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N15/10

CPC classification number: C12N15/1096 ,  C12N15/1065

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US20180340173A1

公开(公告)日：2018-11-29

申请号：US15984905

申请日：2018-05-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N15/10 ,  C12Q1/6809

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US20180195112A1

公开(公告)日：2018-07-12

申请号：US15847408

申请日：2017-12-19

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12Q1/6809 ,  G06F19/18

Abstract: Methods and compositions for maintaining DNA contiguity for sequencing is provided.

公开(公告)号：US20150225786A1

公开(公告)日：2015-08-13

申请号：US14622515

申请日：2015-02-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Claudia Litterst ,  Svilen Tzonev ,  Jeremy Agresti

IPC: C12Q1/68

CPC classification number: C12Q1/6809 ,  C12Q2521/313 ,  C12Q2523/101 ,  C12Q2563/179

Abstract: Methods compositions and kits are provided for performing a chromatin or chromosome conformation capture assay in partitions.

Abstract translation: 提供方法用于在分区中进行染色质或染色体构象捕获测定的组合物和试剂盒。

公开(公告)号：US11834655B2

公开(公告)日：2023-12-05

申请号：US17565249

申请日：2021-12-29

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N5/10 ,  C12Q1/6806 ,  C12N15/10 ,  C12Q1/6869 ,  C12Q1/6809

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6809 ,  C12Q1/6869 ,  C12Q2563/185 ,  C12Q1/6869 ,  C12Q1/6855 ,  C12Q1/6806 ,  C12Q1/6855

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US10676736B2

公开(公告)日：2020-06-09

申请号：US15668321

申请日：2017-08-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N15/10

Abstract: Methods and compositions are provided herein for preparing high-throughput cDNA sequencing libraries.

公开(公告)号：US11685947B2

公开(公告)日：2023-06-27

申请号：US17236743

申请日：2021-04-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12P19/34 ,  C12Q1/6809 ,  G16B20/00 ,  C12Q1/6869

CPC classification number: C12Q1/6809 ,  C12Q1/6869 ,  G16B20/00 ,  C12Q1/6869 ,  C12Q2521/301 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2565/514 ,  C12Q2565/537

Abstract: Methods and compositions for maintaining DNA contiguity for sequencing is provided. For example, a plurality of partitions is provided comprising a bead, a forward primer oligonucleotide cleaved from the bead, the forward primer oligonucleotide having a bead-specific barcode and a 3′ end specific for and complementary to a first or second adaptor; a reverse primer oligonucleotide having a 3′ end complementary to the first or second adaptor, wherein the forward primer 3′ end and the reverse primer 3′ end are complementary to different adaptors selected from the first adaptor and the second adaptor; and fragments of genomic DNA reacted with an adapter-loaded tagmentase such that the DNA fragments comprise breakpoints in the fragments and an inserted adaptor at the break points, wherein the tagmentase binds the breakpoints to form linked DNA segments in the form of DNA segment-first adaptor tagmentase second adaptor-(DNA segment-first adaptor tagmentase second adaptor)n-DNA segment, where n is any integer and “-” indicates a covalent linkage.

公开(公告)号：US10752894B2

公开(公告)日：2020-08-25

申请号：US15984905

申请日：2018-05-21

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Ronald Lebofsky ,  Jeremy Agresti

IPC: C12N15/10 ,  C12Q1/6806 ,  C12Q1/6869 ,  C12Q1/6809

Abstract: Methods and compositions are provided for making and using uniquely tagged target nucleic acid molecules.

公开(公告)号：US20160060621A1

公开(公告)日：2016-03-03

申请号：US14749472

申请日：2015-06-24

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Jeremy Agresti ,  Samantha Cooper ,  George Karlin-Neumann ,  Nick Heredia ,  Ronald Lebofsky

IPC: C12N15/10

CPC classification number: C12N15/1065 ,  C12Q1/6806 ,  C12Q1/6853 ,  C12Q2521/531 ,  C12Q2525/161 ,  C12Q2525/204 ,  C12Q2535/122 ,  C12Q2565/629 ,  C12Q2563/149 ,  C12Q2563/159 ,  C12Q2563/179 ,  C12Q2565/514

Abstract: Methods, compositions, and kits are provided for nucleic acid analysis, including single cell analysis.

Abstract translation: 提供了用于核酸分析的方法，组合物和试剂盒，包括单细胞分析。