公开(公告)号：US11534727B2

公开(公告)日：2022-12-27

申请号：US15290378

申请日：2016-10-11

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Darren Link ,  John Brian Hutchison ,  Michael Samuels ,  Michael Weiner

IPC: B01F3/08 ,  B01F13/00 ,  B01F23/41 ,  B01J19/00 ,  B01L3/00 ,  C40B40/04 ,  C40B50/08 ,  G01N33/50 ,  G01N15/14 ,  B01F25/433 ,  B01F33/3011 ,  B01L7/00 ,  G01N15/10 ,  B01F101/23

Abstract: The present invention generally relates to droplet libraries and to systems and methods for the formation of libraries of droplets. The present invention also relates to methods utilizing these droplet libraries in various biological, chemical, or diagnostic assays.

公开(公告)号：US10724082B2

公开(公告)日：2020-07-28

申请号：US15835697

申请日：2017-12-08

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels

IPC: C12Q1/686

Abstract: The invention generally relates to methods for increasing the amount of DNA available for analysis when using partitioned samples and parallel processing. For example, double-stranded DNA can be dissociated into two single-stranded components, and the single strands partitioned into different droplets prior to analysis. The disclosed methods are useful for performing digital PCR analysis on samples where the target DNA is not in abundance, for example when the sample originates from a body fluid or an FFPE sample.

公开(公告)号：US20240185950A1

公开(公告)日：2024-06-06

申请号：US18538076

申请日：2023-12-13

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Olson ,  Darren R. Link

IPC: G16B20/10 ,  G16B20/00

CPC classification number: G16B20/10 ,  G16B20/00

Abstract: In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.

公开(公告)号：US11193176B2

公开(公告)日：2021-12-07

申请号：US14587365

申请日：2014-12-31

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Darren R. Link ,  Sirshendu Roopom Banerjee

IPC: C12Q1/70

Abstract: The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases.

公开(公告)号：US20240011974A1

公开(公告)日：2024-01-11

申请号：US18359296

申请日：2023-07-26

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/58

CPC classification number: G01N33/58 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11965877B2

公开(公告)日：2024-04-23

申请号：US14874553

申请日：2015-10-05

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Charles Olson ,  Andrew Watson ,  Keith Brown ,  Darren R. Link

IPC: G01N33/53 ,  C12N15/10 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/543 ,  G01N33/58

CPC classification number: G01N33/53 ,  C12N15/1075 ,  C12Q1/6804 ,  C12Q1/6806 ,  C12Q1/6874 ,  C40B50/08 ,  G01N33/532 ,  G01N33/5436 ,  G01N33/58 ,  C12Q2521/10 ,  C12Q2563/179 ,  C12Q2565/30 ,  G01N2458/10

Abstract: The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.

公开(公告)号：US11901041B2

公开(公告)日：2024-02-13

申请号：US14505974

申请日：2014-10-03

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Michael Samuels ,  Jeffrey Olson ,  Darren R. Link

IPC: C12P19/34 ,  G16B20/10 ,  G16B20/00

CPC classification number: G16B20/10 ,  G16B20/00

Abstract: In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.