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公开(公告)号：US11965877B2
公开(公告)日：2024-04-23
申请号：US14874553
申请日：2015-10-05
IPC分类号： G01N33/53 , C12N15/10 , C12Q1/6804 , C12Q1/6806 , C12Q1/6874 , C40B50/08 , G01N33/532 , G01N33/543 , G01N33/58
CPC分类号： G01N33/53 , C12N15/1075 , C12Q1/6804 , C12Q1/6806 , C12Q1/6874 , C40B50/08 , G01N33/532 , G01N33/5436 , G01N33/58 , C12Q2521/10 , C12Q2563/179 , C12Q2565/30 , G01N2458/10
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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公开(公告)号：US11901041B2
公开(公告)日：2024-02-13
申请号：US14505974
申请日：2014-10-03
发明人： Michael Samuels , Jeffrey Olson , Darren R. Link
摘要： In certain aspects, methods of the invention involve performing modification state specific enzymatic reaction of nucleic acid in a sample, determining a value associated with efficiency of the modification state specific enzymatic reaction based on a control, determining an amount of target nucleic acid in the sample, and normalizing the amount of target nucleic acid based on the efficiency value. Based on the normalized amount of target nucleic acid, the method further includes determining whether the normalized amount of target nucleic acid is indicative of a condition.
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公开(公告)号：US20220033919A1
公开(公告)日：2022-02-03
申请号：US17502251
申请日：2021-10-15
IPC分类号： C12Q1/70
摘要： The invention includes methods for determining the presence of a latent viral population by analyzing an RNA population from the virus with digital techniques, such as digital PCR or by sequencing cDNA produced from the RNA. The invention additional includes methods for determining the presence of latent viral populations by detecting and/or quantifying enzymes that are uniquely associated with the virus, e.g., reverse transcriptases.
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公开(公告)号：US11168353B2
公开(公告)日：2021-11-09
申请号：US16400858
申请日：2019-05-01
IPC分类号： C12Q1/6806 , C12Q1/6874 , C40B50/08 , G01N33/543 , G01N33/58 , C12Q1/6804 , C12N15/10
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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公开(公告)号：US20210088424A1
公开(公告)日：2021-03-25
申请号：US17029331
申请日：2020-09-23
发明人： Felix Kleinschmidt , Andrew David Griffiths , Francois Caron , Abdeslam El Harrak , Eric Brouzes , Darren R. Link
摘要： The present invention generally relates to systems and methods to create stable emulsions with low rates of exchange of molecules between microdroplets.
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公开(公告)号：US11768198B2
公开(公告)日：2023-09-26
申请号：US16795467
申请日：2020-02-19
IPC分类号： G01N33/53 , G01N33/532 , C40B50/08 , C12Q1/6874 , G01N33/543 , G01N33/58 , C12Q1/6804 , C12Q1/6806 , C12N15/10
CPC分类号： G01N33/53 , C12N15/1075 , C12Q1/6804 , C12Q1/6806 , C12Q1/6874 , C40B50/08 , G01N33/532 , G01N33/5436 , G01N33/58 , C12Q2521/10 , C12Q2563/179 , C12Q2565/30 , G01N2458/10
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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公开(公告)号：US20220220532A1
公开(公告)日：2022-07-14
申请号：US17700373
申请日：2022-03-21
IPC分类号： C12Q1/6806 , C40B50/08 , C12Q1/6874 , G01N33/543 , G01N33/58 , C12Q1/6804 , C12N15/10
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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公开(公告)号：US12038438B2
公开(公告)日：2024-07-16
申请号：US17509933
申请日：2021-10-25
发明人： Darren R. Link , Michael L. Samuels
IPC分类号： G01N33/573 , B01F23/41 , B01F25/433 , B01F33/3011 , B01J19/00 , B01L3/00 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6851 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/50 , G01N33/542 , G01N33/58 , B01L7/00 , G01N21/64
CPC分类号： G01N33/573 , B01F23/41 , B01F25/433 , B01F25/4336 , B01F33/3011 , B01J19/0046 , B01L3/502761 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , C12Q1/6851 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/5008 , G01N33/542 , G01N33/582 , B01J2219/00286 , B01J2219/00351 , B01J2219/00418 , B01J2219/00479 , B01J2219/00576 , B01J2219/00585 , B01J2219/0059 , B01J2219/00592 , B01J2219/00599 , B01J2219/0065 , B01J2219/00657 , B01J2219/00664 , B01J2219/00702 , B01J2219/0072 , B01J2219/00722 , B01J2219/0074 , B01J2219/00743 , B01L7/52 , B01L2200/027 , B01L2300/0636 , B01L2300/0645 , B01L2300/0654 , B01L2300/0681 , B01L2300/0864 , B01L2300/0867 , B01L2400/0415 , B01L2400/0487 , G01N21/6428 , G01N21/6445 , G01N2500/00
摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
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公开(公告)号：US20220064711A1
公开(公告)日：2022-03-03
申请号：US17529082
申请日：2021-11-17
IPC分类号： C12Q1/6806 , C40B50/08 , C12Q1/6874 , G01N33/543 , G01N33/58 , C12Q1/6804 , C12N15/10
摘要： The invention provides barcode libraries and methods of making and using them including obtaining a plurality of nucleic acid constructs in which each construct comprises a unique N-mer and a functional N-mer and segregating the constructs into a fluid compartments such that each compartment contains one or more copies of a unique construct. The invention further provides methods for digital PCR and for use of barcode libraries in digital PCR.
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公开(公告)号：US20210088519A1
公开(公告)日：2021-03-25
申请号：US17081220
申请日：2020-10-27
发明人： Darren R. Link , Michael L. Samuels
IPC分类号： G01N33/573 , B01F5/06 , B01J19/00 , B01F13/00 , B01L3/00 , B01F3/08 , C40B40/04 , C40B50/08 , C40B60/10 , G01N33/50 , C12Q1/25 , C12Q1/6804 , C12Q1/6818 , C12Q1/6827 , G01N33/542 , G01N33/58
摘要： The invention generally relates to methods for quantifying an amount of enzyme molecules. Systems and methods of the invention are provided for measuring an amount of target by forming a plurality of fluid partitions, a subset of which include the target, performing an enzyme-catalyzed reaction in the subset, and detecting the number of partitions in the subset. The amount of target can be determined based on the detected number.
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