-
公开(公告)号：US09850517B2
公开(公告)日：2017-12-26
申请号：US14039682
申请日：2013-09-27
Applicant: Bio-Rad Laboratories, Inc.
Inventor： Tom McCollum , Paul Patt , Frank Shen , Daniel Y. Chu , Don Flory , Mike Griffin , Xin Heng , Eli Hefner
CPC classification number: C12Q1/06 , G01N15/1434 , G02B21/0008 , G02B21/34
Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.
-
2.发明授权Serial-line-scan-encoded multi-color fluorescence microscopy and imaging flow cytometry 有权串行扫描编码的多色荧光显微镜和成像流式细胞术公开(公告)号：US09091654B2
公开(公告)日：2015-07-28
申请号：US14148600
申请日：2014-01-06
Applicant: Bio-Rad Laboratories, Inc.
CPC classification number: G01N21/6486 , G01J3/02 , G01J3/0208 , G01J3/04 , G01J3/10 , G01J3/36 , G01J3/4406 , G01N15/1425 , G01N15/1429 , G01N15/147 , G01N15/1475 , G01N21/6458 , G01N2021/6419 , G01N2021/6421
Abstract: A system for performing high-speed, high-resolution imaging cytometry includes a scanning region that is illuminated by light including at least first and second wavelength bands. The system also includes a cell transport mechanism that transports a cell through the scanning region such that the cell is illuminated. The system further includes a set of at least one linear light sensor, and an optical system that selectively directs light emitted from the cell to two portions of the linear light sensor set such that emitted light in a third wavelength band is primarily directed to a first portion of the linear light sensor set, and emitted light in a fourth wavelength band is primarily directed to a second portion of the linear light sensor set. The system repeatedly takes readings of light falling on the linear light sensor set while the cell is transported through the scanning region.
Abstract translation: 用于执行高速，高分辨率成像细胞计数的系统包括被包括至少第一和第二波长带的光照射的扫描区域。 该系统还包括传送细胞通过扫描区域使得细胞被照亮的细胞传输机制。 该系统还包括一组至少一个线性光传感器，以及光学系统，其将从单元发射的光选择性地引导到线性光传感器组的两个部分，使得第三波长带中的发射光主要指向第一 线性光传感器组的一部分和第四波长带中的发射光主要指向线状光传感器组的第二部分。 当单元格通过扫描区域传输时，系统重复地读取落在线性光传感器组上的光的读数。
-
3.发明申请SERIAL-LINE-SCAN-ENCODED MULTI-COLOR FLUORESCENCE MICROSCOPY AND IMAGING FLOW CYTOMETRY 有权串行扫描编码多色荧光显微镜和成像流程细胞计数公开(公告)号：US20140186938A1
公开(公告)日：2014-07-03
申请号：US14148600
申请日：2014-01-06
Applicant: Bio-Rad Laboratories, Inc.
IPC: G01N21/64
CPC classification number: G01N21/6486 , G01J3/02 , G01J3/0208 , G01J3/04 , G01J3/10 , G01J3/36 , G01J3/4406 , G01N15/1425 , G01N15/1429 , G01N15/147 , G01N15/1475 , G01N21/6458 , G01N2021/6419 , G01N2021/6421
Abstract: A system for performing high-speed, high-resolution imaging cytometry includes a scanning region that is illuminated by light including at least first and second wavelength bands. The system also includes a cell transport mechanism that transports a cell through the scanning region such that the cell is illuminated. The system further includes a set of at least one linear light sensor, and an optical system that selectively directs light emitted from the cell to two portions of the linear light sensor set such that emitted light in a third wavelength band is primarily directed to a first portion of the linear light sensor set, and emitted light in a fourth wavelength band is primarily directed to a second portion of the linear light sensor set. The system repeatedly takes readings of light falling on the linear light sensor set while the cell is transported through the scanning region.
Abstract translation: 用于执行高速，高分辨率成像细胞计数的系统包括被包括至少第一和第二波长带的光照射的扫描区域。 该系统还包括传送细胞通过扫描区域使得细胞被照亮的细胞传输机制。 该系统还包括一组至少一个线性光传感器，以及光学系统，其将从单元发射的光选择性地引导到线性光传感器组的两个部分，使得第三波长带中的发射光主要指向第一 线性光传感器组的一部分和第四波长带中的发射光主要指向线状光传感器组的第二部分。 当单元格通过扫描区域传输时，系统重复地读取落在线性光传感器组上的光的读数。
-
-
Search Results
3
records
(took 0.012 seconds)
