公开(公告)号：US09850517B2

公开(公告)日：2017-12-26

申请号：US14039682

申请日：2013-09-27

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Tom McCollum ,  Paul Patt ,  Frank Shen ,  Daniel Y. Chu ,  Don Flory ,  Mike Griffin ,  Xin Heng ,  Eli Hefner

IPC: C12Q1/06 ,  G01N15/14 ,  G02B21/34 ,  G02B21/00

CPC classification number: C12Q1/06 ,  G01N15/1434 ,  G02B21/0008 ,  G02B21/34

Abstract: Biological cells in a liquid suspension are counted in an automated cell counter that focuses an image of the suspension on a digital imaging sensor that contains at least 4,000,000 pixels each having an area of 2×2 μm or less and that images a field of view of at least 3 mm2. The sensor enables the counter to compress the optical components into an optical path of less than 20 cm in height when arranged vertically with no changes in direction of the optical path as a whole, and the entire instrument has a footprint of less than 300 cm2. Activation of the light source, automated focusing of the sensor image, and digital cell counting are all initiated by the simple insertion of the sample holder into the instrument. The suspension is placed in a sample chamber in the form of a slide that is shaped to ensure proper orientation of the slide in the cell counter.

公开(公告)号：US09091654B2

公开(公告)日：2015-07-28

申请号：US14148600

申请日：2014-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xin Heng ,  Paul Patt

IPC: G06K9/00 ,  G01N21/64 ,  G01J3/02 ,  G01J3/04 ,  G01J3/10 ,  G01J3/36 ,  G01J3/44 ,  G01N15/14

CPC classification number: G01N21/6486 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/10 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A system for performing high-speed, high-resolution imaging cytometry includes a scanning region that is illuminated by light including at least first and second wavelength bands. The system also includes a cell transport mechanism that transports a cell through the scanning region such that the cell is illuminated. The system further includes a set of at least one linear light sensor, and an optical system that selectively directs light emitted from the cell to two portions of the linear light sensor set such that emitted light in a third wavelength band is primarily directed to a first portion of the linear light sensor set, and emitted light in a fourth wavelength band is primarily directed to a second portion of the linear light sensor set. The system repeatedly takes readings of light falling on the linear light sensor set while the cell is transported through the scanning region.

Abstract translation: 用于执行高速，高分辨率成像细胞计数的系统包括被包括至少第一和第二波长带的光照射的扫描区域。 该系统还包括传送细胞通过扫描区域使得细胞被照亮的细胞传输机制。 该系统还包括一组至少一个线性光传感器，以及光学系统，其将从单元发射的光选择性地引导到线性光传感器组的两个部分，使得第三波长带中的发射光主要指向第一 线性光传感器组的一部分和第四波长带中的发射光主要指向线状光传感器组的第二部分。 当单元格通过扫描区域传输时，系统重复地读取落在线性光传感器组上的光的读数。

公开(公告)号：US20140106989A1

公开(公告)日：2014-04-17

申请号：US14056162

申请日：2013-10-17

Applicant: Bio-Rad Laboratories, Inc., LSG - LSD Division

Inventor： John Barich ,  Renee Lemaire-Adkins ,  David Neaz ,  Ariel Notcovich ,  Paul Patt ,  Ryan Short ,  Steven Swihart ,  Evan Thrush

IPC: G01N21/63

Abstract: Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

Abstract translation: 分析物阵列如电泳后的板状凝胶中的溶质，特别是超过3cm见方和高达25cm见方的高达25cm见方的阵列，通过以下方式成像5cm或更小的距离： 整个阵列并通过基于计算机的缝合技术将子图像拼接在一起，或者通过使用与分析物阵列共同延伸以形成阵列的单个图像的薄膜光响应元件阵列来拼接子图像。

公开(公告)号：US10788423B2

公开(公告)日：2020-09-29

申请号：US15471264

申请日：2017-03-28

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Barich ,  Renee Lemaire-Adkins ,  David Neaz ,  Ariel Notcovich ,  Paul Patt ,  Ryan Short ,  Steven Swihart ,  Evan Thrush ,  Trey Marlowe

IPC: H04N5/357 ,  H04N5/361 ,  G01N21/63 ,  G01N21/64 ,  H04N5/262 ,  H04N5/349 ,  G01N27/447 ,  H04N5/335 ,  G02B21/06

Abstract: Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

公开(公告)号：US10564098B2

公开(公告)日：2020-02-18

申请号：US14056162

申请日：2013-10-17

Applicant: Bio-Rad Laboratories, Inc.

Inventor： John Barich ,  Renee Lemaire-Adkins ,  David Neaz ,  Ariel Notcovich ,  Paul Patt ,  Ryan Short ,  Steven Swihart ,  Evan Thrush ,  Trey Marlowe

IPC: H04N5/361 ,  H04N5/357 ,  H04N5/335 ,  G01N21/64 ,  G01N21/63 ,  H04N5/262 ,  H04N5/349 ,  G01N27/447 ,  G02B21/06

Abstract: Analyte arrays such as solutes in a slab-shaped gel following electrophoresis, and particularly arrays that are in excess of 3 cm square and up to 25 cm square and higher, are imaged at distances of 5 cm or less by either forming sub-images of the entire array and stitching together the sub-images by computer-based stitching technology, or by using an array of thin-film photoresponsive elements that is coextensive with the analyte array to form a single image of the array.

公开(公告)号：US20140186938A1

公开(公告)日：2014-07-03

申请号：US14148600

申请日：2014-01-06

Applicant: Bio-Rad Laboratories, Inc.

Inventor： Xin Heng ,  Paul Patt

IPC: G01N21/64

CPC classification number: G01N21/6486 ,  G01J3/02 ,  G01J3/0208 ,  G01J3/04 ,  G01J3/10 ,  G01J3/36 ,  G01J3/4406 ,  G01N15/1425 ,  G01N15/1429 ,  G01N15/147 ,  G01N15/1475 ,  G01N21/6458 ,  G01N2021/6419 ,  G01N2021/6421

Abstract: A system for performing high-speed, high-resolution imaging cytometry includes a scanning region that is illuminated by light including at least first and second wavelength bands. The system also includes a cell transport mechanism that transports a cell through the scanning region such that the cell is illuminated. The system further includes a set of at least one linear light sensor, and an optical system that selectively directs light emitted from the cell to two portions of the linear light sensor set such that emitted light in a third wavelength band is primarily directed to a first portion of the linear light sensor set, and emitted light in a fourth wavelength band is primarily directed to a second portion of the linear light sensor set. The system repeatedly takes readings of light falling on the linear light sensor set while the cell is transported through the scanning region.

Abstract translation: 用于执行高速，高分辨率成像细胞计数的系统包括被包括至少第一和第二波长带的光照射的扫描区域。 该系统还包括传送细胞通过扫描区域使得细胞被照亮的细胞传输机制。 该系统还包括一组至少一个线性光传感器，以及光学系统，其将从单元发射的光选择性地引导到线性光传感器组的两个部分，使得第三波长带中的发射光主要指向第一 线性光传感器组的一部分和第四波长带中的发射光主要指向线状光传感器组的第二部分。 当单元格通过扫描区域传输时，系统重复地读取落在线性光传感器组上的光的读数。