-
公开(公告)号：US20150274808A1
公开(公告)日：2015-10-01
申请号：US14638215
申请日：2015-03-04
Applicant: BIOGEN MA INC.
Inventor： David Evans , R. Blake Pepinsky , Dingyi Wen , Rashmi Rohit Kshirsagar , Karin Lucas
IPC: C07K16/00
CPC classification number: C07K16/00 , C07K1/1133
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
Abstract translation: 本发明涉及在蛋白质如抗体中预防和消除三硫键的方法。 在一个实施方案中，作为色谱纯化方法的一部分，将蛋白质中的三硫键转化为二硫键。 在另一个实施方案中，通过在这些蛋白质的细胞培养生产期间实施本文所述的方法来抑制蛋白质中三硫键的形成。 在另一个实施方案中，通过本文所述的方法产生单克隆抗体。
-
Patent Agency Ranking
公开(公告)号：US09790533B2
公开(公告)日：2017-10-17
申请号：US15425056
申请日：2017-02-06
Applicant: BIOGEN MA INC.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
CPC classification number: C12P21/00 , A61K39/39591 , A61K2039/505 , C07K1/1133 , C07K16/00 , C07K2317/40 , C07K2317/41 , C07K2317/94 , C12N5/0018 , C12N2500/34
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
-
公开(公告)号：US20170029859A1
公开(公告)日：2017-02-02
申请号：US15291257
申请日：2016-10-12
Applicant: BIOGEN MA INC.
Inventor： Alan Gilbert , Kyle McElearney , Terrence Michael Dobrowsky , Rashmi Rohit Kshirsagar
IPC: C12P21/00 , C07K14/755 , C12N5/00
CPC classification number: C07K14/755 , C12N5/0018 , C12N2500/24 , C12N2500/34 , C12N2510/02 , C12N2511/00 , C12P21/02
Abstract: The present invention pertains to a cell culture medium comprising dextran sulfate or a mixture of dextran sulfate and ferric citrate, and methods of using thereof. The present invention further pertains to a method of producing a protein of interest in a large scale cell culture, comprising supplementing the cell culture with dextran sulfate or a mixture of dextran sulfate and ferric citrate.
Abstract translation: 本发明涉及包含硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物的细胞培养基及其使用方法。 本发明还涉及在大规模细胞培养物中生产感兴趣的蛋白质的方法，其包括用硫酸葡聚糖或硫酸葡聚糖和柠檬酸铁的混合物补充细胞培养物。
-
公开(公告)号：US10590454B2
公开(公告)日：2020-03-17
申请号：US15785268
申请日：2017-10-16
Applicant: Biogen MA Inc.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
-
公开(公告)号：US10308706B2
公开(公告)日：2019-06-04
申请号：US14638215
申请日：2015-03-04
Applicant: BIOGEN MA INC.
Inventor： David Evans , R. Blake Pepinsky , Dingyi Wen , Rashmi Rohit Kshirsagar , Karin Lucas
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
-
公开(公告)号：US20180251805A1
公开(公告)日：2018-09-06
申请号：US15785268
申请日：2017-10-16
Applicant: Biogen MA Inc.
Inventor： Rashmi Rohit Kshirsagar , Alan Gilbert
IPC: C12P21/00 , C12N5/00 , A61K39/395 , C07K16/00 , C07K1/113
CPC classification number: C12P21/00 , A61K39/39591 , A61K2039/505 , C07K1/1133 , C07K16/00 , C07K2317/40 , C07K2317/41 , C07K2317/94 , C12N5/0018 , C12N2500/34
Abstract: The present invention pertains to methods of preventing and eliminating trisulfide bonds in proteins such as antibodies. In one embodiment, trisulfide bonds in proteins are converted to disulfide bonds as part of chromatographic purification procedures. In another embodiment, the formation of trisulfide bonds in proteins is inhibited by implementation of methods described herein during the cell culture production of such proteins. In another embodiment, monoclonal antibodies are produced by the methods described herein.
-
-
-
-
-
Search Results
6
records
(took 0.019 seconds)
