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1.发明授权Method for improved focus control in molecular array scanning by using a symmetrical filter to determine in-focus-distance 失效通过使用对称滤波器来确定对焦距离来改进分子阵列扫描中的聚焦控制的方法公开(公告)号：US07067783B2
公开(公告)日：2006-06-27
申请号：US10261359
申请日：2002-09-30
IPC分类号： G02B7/04
CPC分类号： G02B7/36 , G01N21/6428 , G01N21/6452 , G01N21/6456 , G01N2021/6419 , G01N2021/6421
摘要： Automated methods and systems for determining an in-focus-distance for a position on the surface of a molecular array substrate using a molecular array scanner are provided. A signal from a first position of an array substrate is detected and noise is filtered out of the detected signal using a symmetrical filter to produce an in-focus-distance. In one embodiment, the in-focus-distance is utilized as an estimated in-focus-distance at a second position of the array substrate. The method finds use in maintaining the focus of a light source while scanning the array by the scanner. Also provided are methods of assaying a sample using the methods and systems of the invention, and kits for performing the invention. The subject invention finds use in a variety of different applications, including both genomics and proteomics applications.
摘要翻译： 提供了使用分子阵列扫描仪来确定分子阵列衬底表面上的位置的对焦距离的自动化方法和系统。 检测来自阵列基板的第一位置的信号，并使用对称滤波器将噪声从检测信号中滤出，以产生对焦距离。 在一个实施例中，将聚焦距离用作阵列基板的第二位置处的估计的对焦距离。 该方法用于在扫描仪扫描阵列时保持光源的焦点。 还提供了使用本发明的方法和系统测定样品的方法以及用于实施本发明的试剂盒。 本发明可用于各种不同的应用，包括基因组学和蛋白质组学应用。
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公开(公告)号：US06927389B2
公开(公告)日：2005-08-09
申请号：US10309465
申请日：2002-12-03
申请人： Bo U. Curry , Jayati Ghosh , Kenneth L. Staton
发明人： Bo U. Curry , Jayati Ghosh , Kenneth L. Staton
CPC分类号： G01N21/6452 , G02B7/08
摘要： Optical scanner system approaches are described in which novel focusing approaches are provided. A control algorithm accounts for geometric variation of successive scans in opposite directions across a microarray slide or substrate in order to provide optimized focus. The feedback approach taught may involve PI or PID terms. In either type of control approach, a projected slope of the slide is calculated and followed back and forth outside a scan region of the array in exiting and entering fully adaptive focusing zones, respectively. During turn-around, the system may track a setpoint between the periods of following the extrapolated slope. Also provided are methods of using the subject system in a biopolymer array based application, including genomic and proteomic applications.
摘要翻译： 描述了提供新颖的聚焦方法的光学扫描器系统方法。 控制算法考虑了跨微阵列载玻片或基底的相反方向的连续扫描的几何变化，以便提供优化的焦点。 所教导的反馈方法可能涉及PI或PID术语。 在任一类型的控制方法中，计算滑块的投影斜率并分别在阵列的扫描区域之间来回并进入完全自适应聚焦区域。 在回转期间，系统可以追踪在外推斜率之后的周期之间的设定值。 还提供了在基于生物聚合物阵列的应用中使用主题系统的方法，包括基因组和蛋白质组学应用。
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公开(公告)号：US06902112B2
公开(公告)日：2005-06-07
申请号：US10620918
申请日：2003-07-15
CPC分类号： G01N21/6452 , G01N2021/6463
摘要： Apparatus and method for scanning a surface. An optical system generates a light beam to illuminate a surface. A carrier supports the surface for reciprocating motion with respect to the light beam to form one axis of a raster. A propulsion system moves the carrier at a substantially constant speed and a position sensor provides an output signal representing the surface position with respect to the light beam. A control system responsive to the output signal modulates a sample period reciprocally to carrier speed to achieve substantially constant scan length per sample and to control data acquisition timing.
摘要翻译： 用于扫描表面的装置和方法。 光学系统产生光束照射表面。 载体支撑表面相对于光束往复运动以形成光栅的一个轴。 推进系统以基本恒定的速度移动载体，并且位置传感器提供表示相对于光束的表面位置的输出信号。 响应于输出信号的控制系统将采样周期相互调制为载波速度，以实现每个采样的基本上恒定的扫描长度并且控制数据采集定时。
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公开(公告)号：US06592036B2
公开(公告)日：2003-07-15
申请号：US10124138
申请日：2002-04-16
IPC分类号： G06K714
CPC分类号： G01N21/6452 , G01N2021/6463
摘要： Apparatus and method for scanning a surface. An optical system generates a light beam to illuminate a surface. A carrier supports the surface for reciprocating motion with respect to the light beam to form one axis of a raster. A propulsion system moves the carrier at a substantially constant speed and a position sensor provides an output signal representing the surface position with respect to the light beam. A control system responsive to the output signal modulates a sample period reciprocally to carrier speed to achieve substantially constant scan length per sample and to control data acquisition timing.
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公开(公告)号：US06371370B1
公开(公告)日：2002-04-16
申请号：US09317654
申请日：1999-05-24
IPC分类号： G06K714
摘要： Apparatus and method for scanning a surface. An optical system generates a light beam to illuminate a surface. A carrier supports the surface for reciprocating motion with respect to the light beam to form one axis of a raster. A propulsion system moves the carrier at a substantially constant speed and a position sensor provides an output signal representing the surface position with respect to the light beam. A control system responsive to the output signal modulates a sample period reciprocally to carrier speed to achieve substantially constant scan length per sample and to control data acquisition timing.
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公开(公告)号：US06956203B2
公开(公告)日：2005-10-18
申请号：US10852904
申请日：2004-05-24
CPC分类号： G01N21/6452 , G01N21/274 , G01N21/6428 , G01N21/6458
摘要： A self-calibrating scanning system and method are used in the analysis of biomolecules on a microarray. The self-calibrating scanning system comprises an excitation light source, an optical portion, a detection portion and a calibration portion that includes a calibration apparatus and compensation portion. The calibration apparatus comprises a light source having a highly reproducible or calibrated light based on a preselected or reference light level. The calibration apparatus emits the calibrated light that is measured by the detection portion of the scanning equipment. If the detection components are stable, the components will measure a constant output value for the calibrated light over time. As a detection component changes with time, the output value will change for the same calibrated light. The method comprises the steps of initially calibrating the detection portion of the scanning system and subsequently calibrating the detection portion to compensate for sensitivity changes.
摘要翻译： 自动校准扫描系统和方法用于分析微阵列上的生物分子。 自校准扫描系统包括激发光源，光学部分，检测部分和包括校准装置和补偿部分的校准部分。 校准装置包括基于预选或参考光级别具有高度可再现或校准的光的光源。 校准装置发射由扫描设备的检测部分测量的校准光。 如果检测组件稳定，则组件将随着时间的推移测量校准光的恒定输出值。 当检测组件随时间变化时，相同的校准光的输出值将发生变化。 该方法包括以下步骤：首先校准扫描系统的检测部分，随后校准检测部分以补偿灵敏度变化。
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公开(公告)号：US06930301B2
公开(公告)日：2005-08-16
申请号：US10843249
申请日：2004-05-10
CPC分类号： G01J1/4228 , G01J1/46
摘要： High quantum efficiency point detector system. The system includes a light source generating a light beam having an area and includes a CCD detector with a cell size comparable to the light beam area. The CCD cell may include a single pixel or at least two pixels.
摘要翻译： 高量子效率点检测系统。 该系统包括产生具有区域的光束的光源，并且包括具有与光束区域相当的单元尺寸的CCD检测器。 CCD单元可以包括单个像素或至少两个像素。
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公开(公告)号：US06583424B2
公开(公告)日：2003-06-24
申请号：US09892209
申请日：2001-06-25
IPC分类号： G01N2164
CPC分类号： G01N21/6452 , G01N21/274 , G01N21/6428 , G01N21/6458
摘要： A self-calibrating scanning system and method are used in the analysis of biomolecules on a microarray. The self-calibrating scanning system comprises an excitation light source, an optical portion, a detection portion and a calibration portion that includes a calibration apparatus and compensation portion. The calibration apparatus comprises a light source having a highly reproducible or calibrated light based on a preselected or reference light level. The calibration apparatus emits the calibrated light that is measured by the detection portion of the scanning equipment. If the detection components are stable, the components will measure a constant output value for the calibrated light over time. As a detection component changes with time, the output value will change for the same calibrated light. The method comprises the steps of initially calibrating the detection portion of the scanning system and subsequently calibrating the detection portion to compensate for sensitivity changes.
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公开(公告)号：US06756202B2
公开(公告)日：2004-06-29
申请号：US09846125
申请日：2001-04-30
IPC分类号： C12Q168
CPC分类号： G01N21/6452 , B01J2219/00527 , B01J2219/00529 , B01J2219/00547 , B01J2219/00585 , B01J2219/00596 , B01J2219/00605 , B01J2219/00608 , B01J2219/00659 , B01J2219/00702 , B01J2219/00722 , C40B40/06 , C40B70/00
摘要： A method, apparatus, and computer program product for reading fluorescence signals from an array of chemical moieties (such as different sequence peptides or polynucleotides, for example different DNA sequences). In the method the spatial sequence of scanned locations need not be the same as the temporal sequence. For example, a later illuminated line may be spatially closer to an earlier illuminated line than is a temporally intervening illuminated line.
摘要翻译： 用于从化学部分阵列（例如不同序列肽或多核苷酸，例如不同DNA序列）读取荧光信号的方法，装置和计算机程序产品。 在该方法中，扫描位置的空间序列不必与时间序列相同。 例如，稍后的照明线可以在空间上比在时间上插入的照明线更靠近较早的照明线。
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公开(公告)号：US06518556B2
公开(公告)日：2003-02-11
申请号：US10068419
申请日：2002-02-04
IPC分类号： H01J4014
CPC分类号： G01J1/44 , G01N2021/6463 , H01J43/30
摘要： System for large dynamic range light detection. In one aspect, the system includes a hybrid counting/integrating system for processing a signal from a photomultiplier tube. In another aspect, large dynamic range is achieved in a cascaded detector system utilizing at least one asymmetric beam splitter for delivering a larger fraction of incident light to one photomultiplier tube and for delivering a smaller fraction of the incident light to another photomultiplier tube.
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