公开(公告)号：US20150353619A1

公开(公告)日：2015-12-10

申请号：US14735742

申请日：2015-06-10

Applicant: California Institute of Technology

Inventor： David A. Tirrell ,  Seth Lieblich ,  Katherine Y. Fang ,  Howard C. Zisser

IPC: C07K14/62

CPC classification number: C07K14/62 ,  C07K2319/21

Abstract: In some aspects, the invention relates to an insulin derivative, comprising a cyclic amino acid at position B28 or B29, wherein the cyclic amino acid is not L-proline. In some aspects, the invention relates to a method of making said insulin derivative. In some aspects, the invention relates to a pharmaceutical composition comprising said insulin derivative. In some aspects the invention relates to a method of treating a disease or condition in a subject comprising administering to the subject a composition comprising said insulin derivative.

Abstract translation: 在一些方面，本发明涉及包含位置B28或B29的环状氨基酸的胰岛素衍生物，其中所述环状氨基酸不是L-脯氨酸。 在一些方面，本发明涉及制备所述胰岛素衍生物的方法。 在一些方面，本发明涉及包含所述胰岛素衍生物的药物组合物。 在一些方面，本发明涉及治疗受试者的疾病或病症的方法，其包括对受试者施用包含所述胰岛素衍生物的组合物。

公开(公告)号：US20140255991A1

公开(公告)日：2014-09-11

申请号：US14151686

申请日：2014-01-09

Applicant: California Institute of Technology

Inventor： David A. Tirrell ,  Kristi Lynn Kiick

IPC: C12N9/00 ,  C12P21/00

CPC classification number: C12P21/00 ,  C12N9/003 ,  C12N9/93 ,  C12N15/67 ,  C12P21/02

Abstract: Methods for producing modified polypeptides containing amino acid analogues are disclosed. The invention further provides purified dihydrofolate reductase polypeptides, produced by the methods of the invention, in which the methionine residues have been replaced with homoallylglycine, homoproparglycine, norvaline, norleucine, cis-crotylglycine, trans-crotylglycine, 2-aminoheptanoic acid, 2-butynylglycine and allylglycine.

Abstract translation: 公开了生产含有氨基酸类似物的修饰多肽的方法。 本发明进一步提供了通过本发明方法制备的纯化的二氢叶酸还原酶多肽，其中甲硫氨酸残基已经被均一甘氨酸，高丙氨酸，正缬氨酸，正亮氨酸，顺式 - 巴豆基甘氨酸，反 - 巴豆基甘氨酸，2-氨基庚酸，2-丁炔基甘氨酸 和烯丙基甘氨酸。

公开(公告)号：US20140127777A1

公开(公告)日：2014-05-08

申请号：US14066371

申请日：2013-10-29

Applicant: CALIFORNIA INSTITUTE OF TECHNOLOGY

Inventor： David A. Tirrell ,  Yi Tang

IPC: C12N9/96 ,  C07K2/00

CPC classification number: C12N9/96 ,  C07K1/107 ,  C07K2/00 ,  C07K14/47 ,  C07K14/4705 ,  C12P21/02

Abstract: The present invention provides a method for producing modified stable polypeptides introducing at least one non-natural amino acid into the hydrophobic region of the polypeptide. The thermal and chemical stability of such polypeptides is improved compared to those properties of its corresponding wild type proteins.The invention further provides purified leucine zipper and coiled-coil proteins in which the leucine residues have been replaced with 5,5,5-trifluoroleucines, and the modified proteins so produced demonstrate increased thermal and chemical stability compared to their corresponding wild-type natural proteins.

Abstract translation: 本发明提供了一种制备将至少一个非天然氨基酸引入多肽疏水区的修饰稳定多肽的方法。 与其相应的野生型蛋白质的那些性质相比，这些多肽的热和化学稳定性得到改善。 本发明还提供纯化的亮氨酸拉链和卷曲螺旋蛋白，其中亮氨酸残基已经被5,5,5-三氟色氨酸替代，并且与其相应的野生型天然蛋白相比，所产生的修饰的蛋白质表现出增加的热和化学稳定性 。