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    Polypeptide having cellobiohydrolase activity and uses thereof
    1.
    发明授权
    Polypeptide having cellobiohydrolase activity and uses thereof 有权
    具有纤维二糖水解酶活性的多肽及其用途

    公开(公告)号：US09133448B2

    公开(公告)日：2015-09-15

    申请号：US13578223

    申请日：2011-02-11

    申请人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    发明人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    IPC分类号： C07H21/04 ,  C12N9/42 ,  C12N15/00 ,  C12N1/14 ,  A01H5/00 ,  C13K1/06 ,  C12P7/10

    CPC分类号： C12N9/2437 ,  Y02E50/16

    摘要： The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

    摘要翻译： 本发明涉及包含SEQ ID NO：2所示的氨基酸序列或由SEQ ID NO：1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽，其中所述变体多肽具有 与SEQ ID NO：2中所示的序列具有至少93％的序列同一性，或变体多核苷酸编码与SEQ ID NO：2所示序列具有至少93％序列同一性的多肽。本发明特征在于全长 新基因的编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

    Carbohydrate degrading polypeptide and uses thereof
    2.
    发明授权
    Carbohydrate degrading polypeptide and uses thereof 有权
    碳水化合物降解多肽及其用途

    公开(公告)号：US09163224B2

    公开(公告)日：2015-10-20

    申请号：US13264239

    申请日：2010-04-23

    申请人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    发明人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    IPC分类号： C12N9/00 ,  C12N9/42 ,  C12N9/24 ,  C12N9/34

    CPC分类号： C12N9/2437 ,  C12N9/2402 ,  C12N9/2428 ,  C12Y302/01003 ,  C12Y302/01091

    摘要： The invention relates to a polypeptide having carbohydrate material degrading activity which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

    摘要翻译： 本发明涉及具有碳水化合物材料降解活性的多肽，其包含SEQ ID NO：2所示的氨基酸序列或由SEQ ID NO：1或SEQ ID NO：4的核苷酸序列编码的氨基酸序列，或 变体多肽或其变体多核苷酸，其中所述变体多肽与SEQ ID NO：2所示的序列具有至少96％的序列同一性，或所述变体多核苷酸编码与SEQ ID NO：2中列出的序列具有至少96％的序列同一性的多肽 SEQ ID NO：2。本发明的特征在于新基因的全长编码序列以及全长功能蛋白的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

    CARBOHYDRATE DEGRADING POLYPEPTIDE AND USES THEREOF
    3.
    发明申请
    CARBOHYDRATE DEGRADING POLYPEPTIDE AND USES THEREOF 有权
    碳水化合物降解聚糖及其用途

    公开(公告)号：US20130145501A1

    公开(公告)日：2013-06-06

    申请号：US13264239

    申请日：2010-04-23

    申请人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    发明人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schooneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    IPC分类号： A01H5/00 ,  C12S3/00 ,  C12N15/63 ,  C12N1/15 ,  C12N15/56 ,  C12N9/24

    CPC分类号： C12N9/2437 ,  C12N9/2402 ,  C12N9/2428 ,  C12Y302/01003 ,  C12Y302/01091

    摘要： The invention relates to a polypeptide having carbohydrate material degrading activity which comprises the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1 or SEQ ID NO: 4, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 96% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional protein and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

    摘要翻译： 本发明涉及具有碳水化合物材料降解活性的多肽，其包含SEQ ID NO：2所示的氨基酸序列或由SEQ ID NO：1或SEQ ID NO：4的核苷酸序列编码的氨基酸序列，或 变体多肽或其变体多核苷酸，其中所述变体多肽与SEQ ID NO：2所示的序列具有至少96％的序列同一性，或所述变体多核苷酸编码与SEQ ID NO：2中列出的序列具有至少96％的序列同一性的多肽 SEQ ID NO：2。本发明的特征在于新基因的全长编码序列以及全长功能蛋白的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

    POLYPEPTIDE HAVING CELLOBIOHYDROLASE ACTIVITY AND USES THEREOF
    4.
    发明申请
    POLYPEPTIDE HAVING CELLOBIOHYDROLASE ACTIVITY AND USES THEREOF 有权
    具有环糊精活性的多肽及其用途

    公开(公告)号：US20130061354A1

    公开(公告)日：2013-03-07

    申请号：US13578223

    申请日：2011-02-11

    申请人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schoonneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    发明人： Cornelis Maria Jacobus Sagt ,  Margot Elisabeth Francoise Schoonneveld-Bergmans ,  Johannes Andries Roubos ,  Alrik Pieter Los

    IPC分类号： C12N9/42 ,  C12N15/80 ,  C12N1/15 ,  D21C3/00 ,  C12N9/38 ,  C12N9/96 ,  A01H5/00 ,  C12P19/14 ,  C12N15/56 ,  C12N9/16

    CPC分类号： C12N9/2437 ,  Y02E50/16

    摘要： The invention relates to a polypeptide comprising the amino acid sequence set out in SEQ ID NO: 2 or an amino acid sequence encoded by the nucleotide sequence of SEQ ID NO: 1, or a variant polypeptide or variant polynucleotide thereof, wherein the variant polypeptide has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2 or the variant polynucleotide encodes a polypeptide that has at least 93% sequence identity with the sequence set out in SEQ ID NO: 2. The invention features the full length coding sequence of the novel gene as well as the amino acid sequence of the full-length functional polypeptide and functional equivalents of the gene or the amino acid sequence. The invention also relates to methods for using the polypeptide in industrial processes. Also included in the invention are cells transformed with a polynucleotide according to the invention suitable for producing these proteins.

    摘要翻译： 本发明涉及包含SEQ ID NO：2所示的氨基酸序列或由SEQ ID NO：1的核苷酸序列编码的氨基酸序列或其变体多肽或其变体多核苷酸的多肽，其中所述变体多肽具有 与SEQ ID NO：2中所示的序列具有至少93％的序列同一性，或变体多核苷酸编码与SEQ ID NO：2所示序列具有至少93％序列同一性的多肽。本发明特征在于全长 新基因的编码序列以及全长功能多肽的氨基酸序列和基因或氨基酸序列的功能等同物。 本发明还涉及在工业过程中使用该多肽的方法。 本发明还包括用适合于生产这些蛋白质的根据本发明的多核苷酸转化的细胞。

    Method for Producing a Compound of Interest in a Filamentous Fungal Cell
    7.
    发明申请
    Method for Producing a Compound of Interest in a Filamentous Fungal Cell 有权
    生产丝状真菌细胞感兴趣的化合物的方法

    公开(公告)号：US20080118965A1

    公开(公告)日：2008-05-22

    申请号：US11795824

    申请日：2006-01-24

    申请人： Johannes Andries Roubos ,  Serge Petrus Donkers ,  Hein Stam ,  Noel Nicolaas Maria Elisabeth Van Peij

    发明人： Johannes Andries Roubos ,  Serge Petrus Donkers ,  Hein Stam ,  Noel Nicolaas Maria Elisabeth Van Peij

    IPC分类号： C12P1/02 ,  C12N15/11 ,  C12N15/00 ,  C07H21/00 ,  C12N1/00

    CPC分类号： C12N9/242 ,  C12N9/20 ,  C12N15/80

    摘要： The invention relates to a nucleotide sequence comprising: a synonymous nucleotide coding sequence with optimized codon frequency such that a native codon has been exchanged with a synonymous codon, said synonymous codon encoding the same amino acid as the native codon and having a higher frequency in codon usage as defined in Table 1 than the native codon; and optionally said nucleotide sequence comprises control sequences such as: one translational termination sequence orientated in 5′ towards 3′ direction selected from the following list of sequences: TAAG, TAGA and TAAA, preferably TAAA, and/or one translational initiator coding sequence orientated in 5′ towards 3′ direction selected from the following list of sequences: gctnccyyc, using ambiguity codes for nucleotides: v (A/C/G); n (A/C/G/T), preferably 5′-GCT TCC TTC-3′. The invention further relates to a consensus translational initiator sequence: 5′-mwChkyCAmv-3′, preferably the translational initiator sequence is selected from the list consisting of: 5′-mwChkyCAAA-3′, 5′-mwChkyCACA-3′, and 5′-mwChkyCAAG-3′.

    摘要翻译： 本发明涉及一种核苷酸序列，其包括：具有优化的密码子频率的同义核苷酸编码序列，使得本地密码子已经与同义密码子交换，所述同义密码子编码与天然密码子相同的氨基酸并且在密码子中具有较高的频率 用法如表1所定义而不是本地密码子; 并且任选地所述核苷酸序列包括控制序列，例如：从5个朝向3'方向的5'朝向3个方向的一个翻译终止序列，其选自以下的序列：TAAG，TAGA和TAAA，优选TAAA，和/或一个定向于 从以下序列列表中选择5'朝向3'方向：gctnccyyc，使用核苷酸的模糊码：v（A / C / G）; n（A / C / G / T），优选5'-GCT TCC TTC-3'。 本发明还涉及一个共有的翻译启动子序列：5'-mwChkyCAmv-3'，优选的是翻译起始子序列选自：5'-mwChkyCAAA-3'，5'-mwChkyCACA-3'和5 '-mwChkyCAAG-3'。

    Method for achieving improved polypeptide expression
    8.
    发明授权
    Method for achieving improved polypeptide expression 有权
    实现改良多肽表达的方法

    公开(公告)号：US08812247B2

    公开(公告)日：2014-08-19

    申请号：US12306678

    申请日：2007-06-15

    申请人： Johannes Andries Roubos ,  Noël Nicolaas Maria Elisabeth Van Peij

    发明人： Johannes Andries Roubos ,  Noël Nicolaas Maria Elisabeth Van Peij

    IPC分类号： G06F7/00 ,  C12N15/81 ,  C12N15/80 ,  C12N15/67

    CPC分类号： C12N15/67 ,  C12N9/242 ,  C12N9/92 ,  C12N15/80 ,  C12N15/81 ,  C12N2800/22

    摘要： The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimization of single codon fitness and/or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence for a predetermined amino acid sequence, the coding sequence being optimised with respect to single codon fitness and/or codon pair fitness for a given host in the methods of the invention, to host cells comprising such nucleic acids and to methods for producing polypeptides and other fermentation products in which these host cells are used.

    摘要翻译： 本发明涉及用于在给定宿主细胞中表达的蛋白质编码序列的优化方法。 该方法应用遗传算法来优化编码预定氨基酸序列的单个密码子适合度和/或密码子对适应性序列。 在算法中重复生成新的序列变体并随后选择拟合变体，直到变体编码序列达到单个密码子适应度和/或密码子对适应度的最小值。 本发明还涉及一种包括处理器和存储器的计算机，所述处理器被布置为从存储器读取和写入存储器，所述存储器包括数据和指令，所述数据和指令被布置为向处理器提供执行用于优化单个密码子的遗传算法的能力 健身和/或密码子对健身。 本发明还涉及包含用于预定氨基酸序列的编码序列的核酸，所述编码序列针对本发明方法中给定宿主的单个密码子适合度和/或密码子对适应性优化至包含 这种核酸以及用于产生其中使用这些宿主细胞的多肽和其它发酵产物的方法。

    METHOD FOR ACHIEVING IMPROVED POLYPEPTIDE EXPRESSION
    10.
    发明申请
    METHOD FOR ACHIEVING IMPROVED POLYPEPTIDE EXPRESSION 有权
    用于实现改进的多肽表达的方法

    公开(公告)号：US20090286280A1

    公开(公告)日：2009-11-19

    申请号：US12306678

    申请日：2007-06-15

    申请人： Johannes Andries Roubos ,  Noël Nicolaas Maria Elisabeth Van Peij

    发明人： Johannes Andries Roubos ,  Noël Nicolaas Maria Elisabeth Van Peij

    IPC分类号： C12P21/00 ,  C07H21/00 ,  C12N1/21 ,  C12P1/00 ,  G06F19/00

    CPC分类号： C12N15/67 ,  C12N9/242 ,  C12N9/92 ,  C12N15/80 ,  C12N15/81 ,  C12N2800/22

    摘要： The present invention relates to methods of optimization of a protein coding sequences for expression in a given host cell. The methods apply genetic algorithms to optimise single codon fitness and/or codon pair fitness sequences coding for a predetermined amino acid sequence. In the algorithm generation of new sequence variants and subsequent selection of fitter variants is reiterated until the variant coding sequences reach a minimum value for single codon fitness and/or codon pair fitness. The invention also relates to a computer comprising a processor and memory, the processor being arranged to read from and write into the memory, the memory comprising data and instructions arranged to provide the processor with the capacity to perform the genetic algorithms for optimisation of single codon fitness and/or codon pair fitness. The invention further relates to nucleic acids comprising a coding sequence for a predetermined amino acid sequence, the coding sequence being optimised with respect to single codon fitness and/or codon pair fitness for a given host in the methods of the invention, to host cells comprising such nucleic acids and to methods for producing polypeptides and other fermentation products in which these host cells are used.

    摘要翻译： 本发明涉及用于在给定宿主细胞中表达的蛋白质编码序列的优化方法。 该方法应用遗传算法来优化编码预定氨基酸序列的单个密码子适合度和/或密码子对适应性序列。 在算法中重复生成新的序列变体并随后选择拟合变体，直到变体编码序列达到单个密码子适应度和/或密码子对适应度的最小值。 本发明还涉及一种包括处理器和存储器的计算机，所述处理器被布置为从存储器读取和写入存储器，所述存储器包括数据和指令，其被布置为向处理器提供执行遗传算法以优化单个密码子的能力 健身和/或密码子对健身。 本发明还涉及包含用于预定氨基酸序列的编码序列的核酸，所述编码序列针对本发明方法中给定宿主的单个密码子适合度和/或密码子对适应性优化至包含 这种核酸以及用于产生其中使用这些宿主细胞的多肽和其它发酵产物的方法。