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    MUTANT PENICILLIN G ACYLASES
    3.
    发明申请
    MUTANT PENICILLIN G ACYLASES 有权

    公开(公告)号：US20110256585A1

    公开(公告)日：2011-10-20

    申请号：US13141877

    申请日：2009-12-22

    申请人： Jan Metske Van Der Laan ,  Harold Monro Moody ,  Thomas Van Der Does

    发明人： Jan Metske Van Der Laan ,  Harold Monro Moody ,  Thomas Van Der Does

    IPC分类号： C12P37/00 ,  C07H21/04 ,  C12N1/21 ,  C12N15/63 ,  C12P35/00 ,  C12N9/78

    CPC分类号： C12N9/84 ,  C12P35/04 ,  C12P37/04

    摘要： The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase having the amino acid sequence depicted in SEQ ID No: 1.

    摘要翻译： 本发明涉及源自野生型青霉素G酰基转移酶的突变型原核青霉素G酰基转移酶，其特征在于突变体在选自氨基酸位置A3，A77的一个或多个氨基酸位置具有氨基酸取代 ，A90，A144A192，B24，B109，B148，B313，B460和B488，其具有SEQ ID No：1所示的氨基酸序列的大肠杆菌青霉素G酰基转移酶的氨基酸编号。

    Pentose sugar fermenting cell
    4.
    发明授权
    Pentose sugar fermenting cell 有权
    戊糖发酵细胞

    公开(公告)号：US08399215B2

    公开(公告)日：2013-03-19

    申请号：US12867927

    申请日：2009-03-05

    申请人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

    发明人： Paul Klaassen ,  Jan Metske Van Der Laan ,  Bianca Elisabeth Maria Gielesen ,  Gijsberdina Pieternella Van Suylekom

    IPC分类号： C12P1/00 ,  C12N1/00

    CPC分类号： C12N9/92 ,  C12P7/10 ,  Y02E50/16

    摘要： The invention relates to a cell which comprises a nucleotide sequence encoding a xylose isomerase, wherein the amino acid sequence of the xylose isomerase has at least about 70% sequence identity to the amino acid sequence set out in SEQ ID NO: 3 and wherein the nucleotide sequence is heterologous to the host. The cell of the invention may be used in a process for producing a fermentation product, such as ethanol. Such a process may comprise fermenting a medium containing a source of xylose with a cell of the invention such that the cell ferments xylose to the fermentation product.

    摘要翻译： 本发明涉及包含编码木糖异构酶的核苷酸序列的细胞，其中木糖异构酶的氨基酸序列与SEQ ID NO：3所示的氨基酸序列具有至少约70％的序列同一性，其中所述核苷酸 序列与宿主异源。 本发明的细胞可以用于生产发酵产物如乙醇的方法。 这样的方法可以包括用本发明的细胞发酵含有木糖来源的培养基，使得细胞向发酵产物发酵木糖。

    Mutant penicillin G acylases
    8.
    发明授权
    Mutant penicillin G acylases 有权
    突变型青霉素G酰基转移酶

    公开(公告)号：US08541199B2

    公开(公告)日：2013-09-24

    申请号：US13141877

    申请日：2009-12-22

    申请人： Jan Metske Van Der Laan ,  Harold Monro Moody ,  Richard Kerkman ,  Thomas Van Der Does

    发明人： Jan Metske Van Der Laan ,  Harold Monro Moody ,  Richard Kerkman ,  Thomas Van Der Does

    IPC分类号： C12P1/00 ,  C12N9/84 ,  C12N1/20 ,  C07H21/04

    CPC分类号： C12N9/84 ,  C12P35/04 ,  C12P37/04

    摘要： The present invention relates to a mutant prokaryotic penicillin G acylase derived from a wild-type penicillin G acylase characterized in that the mutant is having an amino acid substitution at one or more amino acid positions selected from the group consisting of amino acid positions A3, A77, A90, A144 A192, B24, B109, B148, B313, B460 and B488 according to the amino acid numbering of the Escherichia coli penicillin G acylase having the amino acid sequence depicted in SEQ ID No: 1.

    摘要翻译： 本发明涉及源自野生型青霉素G酰基转移酶的突变型原核青霉素G酰基转移酶，其特征在于突变体在选自氨基酸位置A3，A77的一个或多个氨基酸位置具有氨基酸取代 ，A90，A144A192，B24，B109，B148，B313，B460和B488，其具有SEQ ID No：1所示的氨基酸序列的大肠杆菌青霉素G酰基转移酶的氨基酸编号。

    Production of β-Lactam antibiotics
    9.
    发明授权
    Production of β-Lactam antibiotics 有权
    生产＆bgr - 内酰胺抗生素

    公开(公告)号：US08293511B2

    公开(公告)日：2012-10-23

    申请号：US12444108

    申请日：2007-10-02

    申请人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    发明人： Marcus Hans ,  Roelof Ary Lans Bovenberg ,  Paul Klaassen ,  Rémon Boer ,  Jan Metske Van Der Laan

    IPC分类号： C12N9/00 ,  C12P37/00

    CPC分类号： C12N9/0004

    摘要： The present invention describes a process for the production of an N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl β-lactam antibiotic comprising an IPNS-catalysed conversion of a precursor tripeptide hydroxyphenylglycyl-cysteinyl-valine (HpgCV) or phenylglycyl-cysteinyl-valine (PgCV), respectively, to the N-hydroxyphenylglycyl or the N-phenylglycyl β-lactam antibiotic, respectively. The tripeptide HpgCV or the tripeptide PgCV may further be prepared by contacting the amino acids hydroxyphenylglycine (Hpg) or phenylglycine (Pg), cystein (C) and valine (V) with a non-ribosomal peptide synthetase (NRPS) to effect formation of the tripeptide HpgCV or the tripeptide PgCV, the NRPS comprising a first module M1 specific for Hpg or Pg, a second module M2 specific for C and a third module M3 specific for V An IPNS is further provided having an improved activity in this conversion, as well as an NRPS catalysing the formation of the tripeptides. Also a host cell is provided capable of fermentatively producing β-lactam antibiotics with N-α-amino-hydroxyphenylacetyl or an N-α-aminophenylacetyl side chains.

    摘要翻译： 本发明描述了一种制备N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯基乙酰基 - 内酰胺抗生素的方法，其包括前体三肽羟基苯基甘氨酰 - 半胱氨酰 - 缬氨酸（HpgCV）或苯基甘氨酰 - 半胱氨酰缬氨酸（PgCV）分别转化为N-羟基苯基甘氨酰或N-苯基甘氨酰和β-内酰胺抗生素。 三肽HpgCV或三肽PgCV可以通过使氨基酸羟基苯基甘氨酸（Hpg）或苯基甘氨酸（Pg），半胱氨酸（C）和缬氨酸（V）与非核糖体肽合成酶（NRPS）接触来进一步制备，以形成 三肽HpgCV或三肽PgCV，NRPS包括特定于Hpg或Pg的第一模块M1，特定于C的第​​二模块M2和特定于V An IPNS的第三模块M3，还具有在该转换中具有改进的活性 作为催化三肽形成的NRPS。 还提供了能够用N-α-氨基 - 羟基苯基乙酰基或N-α-氨基苯乙酰侧链发酵生产β-内酰胺抗生素的宿主细胞。